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Environmental Internship Program - 2019 Booklet

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Ezra Zimble ’22<br />

UNDECLARED<br />

CLIMATE CHANGE AND<br />

ENVIRONMENTAL SCIENCE<br />

PROJECT TITLE<br />

Examining Biological<br />

Nitrogen Fixation Rates in<br />

New Jersey Forest<br />

Samples*<br />

ORGANIZATION(S)<br />

Zhang Lab, Department<br />

of Geosciences,<br />

Princeton University<br />

LOCATION(S)<br />

Princeton, New Jersey<br />

MENTOR(S)<br />

Xinning Zhang, Assistant<br />

Professor of Geosciences<br />

and the Princeton<br />

<strong>Environmental</strong> Institute;<br />

Romain Darnajoux,<br />

Postdoctoral Research<br />

Fellow, Geosciences<br />

I studied nitrogen fixation in New Jersey<br />

forests as an extension of research conducted in<br />

Canada by my supervisor, Romain Darnajoux.<br />

We applied his work in boreal forests to New<br />

Jersey's temperate forests by investigating<br />

whether nitrogen-fixing bacteria were present<br />

and, if so, the conditions that favor nitrogen<br />

fixation. My jobs included collecting samples<br />

such as mosses, tree bark and soil, then<br />

analyzing them in the lab. Over the course of<br />

the internship, I gained proficiency in using lab<br />

equipment such as gas chromatography and<br />

learned about the safe handling of chemicals.<br />

Additionally, my supervisor and I discussed<br />

experimental design at length before our weekly<br />

experiments and proper data analysis afterward.<br />

I plan to incorporate the sensitivity to detail in<br />

experiment design and analysis I learned to my<br />

future research at Princeton.<br />

* This internship is connected to the PEI Climate<br />

and Energy Grand Challenges project, “Controls on<br />

Alternative N 2<br />

Fixation.”<br />

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