B. Murienne - Master Project Thesis - Infoscience - EPFL

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3.11 Acquisition setup Materials -MiCam Ultima Power box -MiCam Ultima Acquisition box -Computer with Ultima software -Voltage converter Assembly Optical setup for cardiomyocyte monolayer Acquisition setup CMOS camera Electrodes, oxygenation and temperature setups 30 Legend: LED light source 1× lens Emission filter Dichroic miror Excitation light Emission light Stretcher Figure 16. Optical setup for cardiomyocyte monolayers. The acquisition setup is shown in Figure 17. The stimulation setup is linked to the acquisition setup in order for the pacing stimulus to be recorded.
Stimulation and acquisition setups for cardiomyocyte monolayer Electrodes Computer Isolator Voltage converter MiCam Ultima Acquisition box Stimulator 31 Camera, Optical setup Legend: Power box Acquisition Stimulation Power Figure 17. Stimulation and acquisition setups for cardiomyocyte monolayers. 3.12 Extraction of activation time, repolarization, APD and CV values Materials -Matlab scripts Method First, the global activation time (time for activation of the whole imaged area for the cardiomyocyte monolayer model) has to be calculated. For this purpose, the 98-2 percentiles (98 percentile minus 2 percentile) of the activation times is calculated for each beat of each run. Then, for each run the mean of the 98-2 percentiles obtained is taken, giving one activation time value per run. For the calculation of repolarization and APD values, the median of the values obtained for each beat of each run is calculated. Then, for each run the mean of the values obtained is taken, giving one repolarization and one APD value per run. As for the conduction velocity, it is calculated for each beat of each run, from the conduction velocity vector field maps obtained. First, the median of the magnitude of all the velocity vectors contained in a particular region of interest is calculated for all maps of each run. Then, the mean of all the resulting vectors is taken and gives a conduction velocity value for each run.
Page 1 and 2: ECOLE POLYTECHNIQUE FEDERALE DE LAU
Page 3 and 4: Abstract Stretch has been shown to
Page 5 and 6: the L-type Ca2+ channels in guinea-
Page 7 and 8: hythmicity of the ectopic foci can
Page 9 and 10: Figure 2. Main waves of a normal el
Page 11 and 12: direct entry of Ca2+ via stretch-ac
Page 13 and 14: membrane, which forms the bottom of
Page 15 and 16: when bound to a phospholipid bilaye
Page 17 and 18: 2.6 Post-processing of the optical
Page 19 and 20: a. b. c. Figure 10. a. Activation m
Page 21 and 22: Maps of the conduction velocity mag
Page 23 and 24: After the coating process, the wafe
Page 25 and 26: 24 hours later, the preparation is
Page 27 and 28: 3.7 Immunostaining Materials -1X PB
Page 29: 3.9 Stretcher calibration Materials
Page 33 and 34: 4.2 Staining and optical signal rec
Page 35 and 36: cardiomyocytes. In fact, gaps or ba
Page 37 and 38: Figure 24. Non-paced (top signal) a
Page 39 and 40: propagation along the micropatterns
Page 41 and 42: that the cardiomyocytes were able t
Page 43 and 44: transverse axis and 4% stretch appl
Page 45 and 46: 5. Discussion The results obtained
Page 47 and 48: a little higher than the ones found
Page 49 and 50: 6. Conclusion Studying the effect o
Page 51 and 52: [24] Hutton P, Cooper GM, III FMJ,
Page 53: Acknowledgments Thank you to Dr. Mc

B. Murienne - Master Project Thesis - Infoscience - EPFL

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