Manual - New England Biolabs
Manual - New England Biolabs
Manual - New England Biolabs
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Luciferase Activity (RLU)<br />
25,000<br />
20,000<br />
15,000<br />
10,000<br />
5,000<br />
RSL1<br />
Addition<br />
RSL1 (500 nM)<br />
DMSO Control<br />
RSL1<br />
Removal<br />
RSL1<br />
Addition<br />
RSL1<br />
Removal<br />
0<br />
0 12 24 36 48<br />
Hours<br />
60 72 84 96<br />
Figure 6: Rapid ON:OFF switch response to two cycles of addition and removal of RSL1.<br />
NIH 3T3 cells were co-transfected with pNEBR-R1 and pNEBR-X1 expressing a destabilized<br />
firefly luciferase and induced with RSL1 followed by removal of RSL1 at 24 hours. A second<br />
cycle of addition/removal of RSL1 results in a new ON:OFF switch response. To remove RSL1<br />
and turn off expression, cells were washed and RSL1-free media was added at the times<br />
indicated. The blue line represents induced expression. The red line represents uninduced<br />
expression.<br />
11
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