Manual - New England Biolabs
Manual - New England Biolabs
Manual - New England Biolabs
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18<br />
B.<br />
PshA I 3640<br />
BfuA I - BspM I 3355<br />
Sac II 2890<br />
5XRE<br />
TATA box<br />
...CTCCGAGCGGAGTACTGTCCTCCGAGCGGAGACTCTAGAGGGTATATAATGGGGGCCGTCAGCT<br />
. . . .<br />
RheoSwitch<br />
.<br />
5820 5840<br />
predicted<br />
R-X1 primer (#S1280S)<br />
transcription<br />
start<br />
SacI ApaI NarI HindIII EcoRV<br />
ACTACCAGAGCTCATGGGCCCGTGCAATTGAAGCCGGCTGGCGCCAAGCTTCTCTGCAGGATAT<br />
. . . . . .<br />
5880 5900 5920<br />
XhoI BssHII<br />
BamHI PacI FseI PspXI NotI NheI AscI<br />
CTGGATCCACGAATTCTTAATTAAGGCCGGCCTCGAGCGGCCGCTAGCGATCGCGGCGCGCCAC<br />
. . . . . . .<br />
5940 5960 5980 6000<br />
StuI T7 minimal primer<br />
BsrGI SphI SalI<br />
(#S1272S)<br />
GCGTCTCCGGATGTACAGGCATGCGTCGACCCTCTAGTCAAGGCCTATAGTGAGTCGTATTACG...<br />
. . . . . .<br />
6020 6040 6060<br />
Figure 6B: pNEBR-X1Hygro multiple cloning sites (MCS). To minimize uninduced expression,<br />
a short sequence containing the TATA box separates the end of the 5XRE from the<br />
predicted transcription start. Only unique restriction enzyme sites are shown.
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