Evaluation of MassCleave™ Technology For Diagnostic Mutation ...
Evaluation of MassCleave™ Technology For Diagnostic Mutation ...
Evaluation of MassCleave™ Technology For Diagnostic Mutation ...
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PCR<br />
T7-<br />
MassCleave MassCleave<br />
chemistry<br />
Universal forward primer<br />
with T7 promoter tag<br />
C specific<br />
cleavage product<br />
<strong>For</strong>ward transcription<br />
with T7 promoter<br />
U specific<br />
cleavage product<br />
C specific<br />
cleavage product<br />
Reverse transcription<br />
with T7 promoter<br />
Mass spectrometry and comparison to predicted patterns<br />
Universal reverse primer<br />
with T7 promoter tag -T7<br />
U specific<br />
cleavage product<br />
Ref: A strategy for the rapid discovery <strong>of</strong> disease markers using the MassARRAY system.<br />
Rodi CP, Darnh<strong>of</strong>er-Patel B, Stanssens P, Zabeau M, van den Boom D. Biotechniques. 2002 Jun;Suppl:62-6, 68-9.
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