Leica TCS SP5 Confocal Laser Scanning Microscope User Guide ...

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Page 43 The Scale Bar, Overlay, and Maximum projection tools were used to create this set of images. To save this set of images and lines to the virtual window, right click and click on . INSERTING A SCALE BAR
Page 44 This orthogonal (X and Y) edge views of vertical planes through a Z-Series was constructed through the Orthogonal View icon. X Y Lines can be deleted by right-clicking and selecting ORTHOGONAL VIEWS
Page 1 and 2: Leica TCS SP5 Confocal Laser Scanni
Page 3 and 4: Page 20 The Main User Interface app
Page 5 and 6: Acquiring images by simultaneous sc
Page 7 and 8: Page 24 9. Click on and / or UV 10
Page 9 and 10: ALEXA 488 Excitation ALEXA 488 Emis
Page 11 and 12: 13. Use the z - Position knob on th
Page 14 and 15: 15. Click on an image in the image
Page 16 and 17: 18. Adjust the Gain so that so that
Page 18 and 19: LINE AND FRAME AVERAGING Image grai
Page 20 and 21: 27. 26. While scanning, turn the Z-
Page 22 and 23: The Z - Volume (depth) in microns b
Page 25: To project an image or series; 34.
Page 30 and 31: Software Key (Dongle) LAS - AF CONF

Leica TCS SP5 Confocal Laser Scanning Microscope User Guide ...

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