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Leica TCS SP5 Confocal Laser Scanning Microscope User Guide ...

Leica TCS SP5 Confocal Laser Scanning Microscope User Guide ...

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Page<br />

25<br />

EXCITATION LINES FOR:<br />

SPECTRAL GRAPH<br />

FITC TRITC CY5<br />

Active lasers (FITC – TRITC – Cy5 in this case) are indicated by thin<br />

lines representing excitation wavelengths on the spectral graph.<br />

11. Select and Activate the PMTs (PhotoMultiplier Tubes) by clicking on<br />

(check mark) their Active buttons. Gray shadows leading from the PMT<br />

slider bars to the activation panels will appear confirming PMT activation.

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