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Leica TCS SP5 Confocal Laser Scanning Microscope User Guide ...

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Configuring the system for Fluorescence<br />

Page<br />

21<br />

3.<br />

4.<br />

5.<br />

3. Click on in the<br />

LAS AF window and the<br />

configuration window will open.<br />

4. Click on and the <strong>Laser</strong><br />

Switch Window will open.<br />

5. Check mark the lasers that you<br />

intend to use – (The 405 Diode<br />

laser is for UV [ e.g. DAPI ].<br />

LASER SWITCH WINDOW<br />

6. When using the Argon laser,<br />

set the power slider to 25%.<br />

~ 25%<br />

6.<br />

POWER SLIDER

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