182 locations may be the possible reasons <strong>for</strong> these variations in incidence rates <strong>of</strong> Aeromonas in poultry. One <strong>of</strong> the 50 milk samples screened turned out to be positive <strong>for</strong> Aeromonas. This also gave positive result with all three <strong>methods</strong> viz., duplex-<strong>PCR</strong> (Fig. 1), I-<strong>ELISA</strong> as well as <strong>cultural</strong> method. The incidence rate <strong>of</strong> Aeromonas in milk thus was 2.0% which was comparable to the isolation rate <strong>of</strong> 2.85%, 5.56% <strong>and</strong> 4.0% reported by Agarwal et al. (2000), Kumar et al. (2001) <strong>and</strong> Porteen (2004), respectively. The study revealed that there was a good correlation between duplex-<strong>PCR</strong> <strong>and</strong> <strong>cultural</strong> method but indirect-<strong>ELISA</strong>, though detecting all naturally contaminated chicken <strong>and</strong> milk samples, was not able to detect Aeromonas below the concentrations <strong>of</strong> 10 3 cells/ml or g in the spiking studies. This limits the use <strong>of</strong> indirect-<strong>ELISA</strong> as a fool-pro<strong>of</strong> detection method <strong>for</strong> aeromonads in foods when compared to <strong>PCR</strong>. 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