10. Microbiology - Invitrogen

MIC RELI TM
Fig. 1.
AutoRELI
– Automated
reverse line
blot
Fig. 2.
Principle of
the RELI
method
Fig. 3.
Position of
probes on
membrane
Fig. 5: Diagrammatic representation of the
principle of the MIC RELI assay.
IC
Ordering Information
MIC RELI TM
Product Volume Product No.
MIC RELI TM 4 Meningococcus 50 tests 750.01
Strip Detection Reagent Kits
A
C
X
Z/29E
IC - Internal PCR inhibition
and hybridisation control
Fig. 4.
Results of MIC RELI typing for eight
meningococcal isolates.
1 - Serogroup A
2 – Serogroup X
3 – Serogroup C
4 – Serogroup W135
5 – Serogroup C
Ref
CtrA
B
W135
6 – Serogroup Y
7 – Serogroup B
8 – Serogroup B
9 – No Template Control
10 – No Amplification Control
Y
NEW
112
New product
Dynal MIC RELI is a new product for Dynal Microbiology. It is a molecular test
that utilises a generic PCR amplification with biotinylated primers that flank
the specific DNA target region of interest. This is followed by hybridisation
of the resulting amplicon to an array of Sequence Specific Oligonucleotide
probes immobilised on a membrane. The immobilisation of these probes
is referred to as a Reverse Line blot and thus gives rise to the acronym
“RELI”.
Under appropriate wash conditions, the probes remain bound only to their
complementary sequences in the amplified DNA. The sensitivity of the system
enables single nucleotide differences to be distinguished. The presence of
the amplicon/probe complex is detected using a simple colorimetric reaction
and appears as a blue precipitate (fig. 5, section 2).
The first product in this range is MIC RELI 4 Meningococcus (fig. 5, section
3). This kit provides a presence/absence test for Neisseria meningitidis and
identification of the common serogroups (fig. 5, section 4). The assay can
be performed manually or can be automated using the Dynal AutoRELI 48
instrument (fig. 5, section 1).
50 tests
250 tests
1000 tests
802.01
802.250
802.999
AutoRELI 48 1 unit 899.05
www.dynalbiotech.com