10. Microbiology - Invitrogen

10. Microbiology
Food Microbiology 110
Water Testing 111
MIC RELI TM 112
www.dynalbiotech.com
Visit www.dynalbiotech.com for the latest product up-dates and references.
109

Food Microbiology
Fig. 1: Scanning electronmicrograph of E.coli 0157
attached to Dynabeads ® . Enlarged 12,500 x.
Fig. 2: BeadRetriever TM
Ordering Information
Microbiology
The Dynabeads ® Microbiology product range for rapid detection of bacteria
and protozoa using immunomagnetic separation has become widely accepted
and routinely used in the Food & Environmental Microbiology Laboratories
around the world. Validations have been awarded from AOAC, USEPA, AFNOR,
DETR and other government bodies worldwide.
http://www.dynalbiotech.com
Food Microbiology
Dynabeads ® for food microbiology remove the need for traditional media-
based selective enrichment. They offer increased sensitivity, down to 1 viable
organism per 25 g of sample or 100 organisms per ml of pre-enrichment
broth, and a significant reduction in background flora. The Dynabeads ® are
2.8 µm in diameter and covalently coated with affinity-purified antibodies
against specific surface markers on the microorganisms. The method
is rapid & simple to perform. It can be performed manually using Dynal
Magnets & Mixers (see chapter 8) or the process can be automated using
the BeadRetriever TM bench top instrument.
Product Volume Product No.
BeadRetriever TM 1 unit 159.50
BeadRetriever TM Tubes & Tips 240 tests 159.51
BeadRetriever TM Tube Rack 1 unit 159.52
Dynabeads ® anti-Salmonella 5 ml / 250 tests 710.02
Dynabeads ® anti-E. coli O157
110
1 ml / 50 tests
5 ml / 250 tests
710.03
710.04
Dynabeads ® anti-Listeria 5 ml / 250 tests 710.06
Dynabeads ® anti-EPEC/VTEC O145 2 ml / 100 tests 710.07
Dynabeads ® anti-EPEC/VTEC O111 2 ml / 100 tests 710.09
Dynabeads ® anti-EPEC/VTEC O103 2 ml / 100 tests 710.11
Dynabeads ® anti-EPEC/VTEC O26 2 ml / 100 tests 710.13
www.dynalbiotech.com

Water Testing
Dynal Biotech is the world leader in the water industry in sample preparation
for Cryptosporidium and Giardia and Cryptosporidium (GC) combination
testing. Dynabeads ® anti-Cryptosporidium and GC Combo are the gold
standard method validated by the US Environmental Protection Agency for
selective enrichment of these protozoa (US EPA Method 1622 &1623; UK DETR
Water Supply Regulation 2000 SI No. 3184 England & SI No. 3911 Wales). The
Dynabeads ® are covalently coated with affinity-purified antibodies against
specific surface markers on the oocyst/cyst. This protocol processes a 10 ml
contaminated water sample concentrate (obtained from any untreated (raw)
or treated water) to produce one clean microscope slide with significantly
reduced background debris. Recoveries of oocyst/cysts are regularly greater
than 90%.
The most recent addition to Dynal Biotech’s water testing product portfolio is
Dynabeads ® anti-Legionella. This product is for rapid, selective separation of
Legionella species from water samples and water concentrates. The method
can be performed manually using Dynal Magnets & Mixers (see chapter
8) or the process can be automated using the BeadRetriever TM bench top
instrument.
Ordering Information
www.dynalbiotech.com
111
Water Testing
Fig. 3: A water sample without Dynabeads ® anti-
Cryptosporidium selective enrichment which has
been stained for detection of Cryptosporidium
oocysts .
Fig. 4: A water sample after Dynabeads ® anti-
Cryptosporidium selective enrichment which has
been stained for detection of Cryptosporidium
oocysts .
Product Volume Product No.
Dynabeads ® anti-Cryptosporidium Kit
Dynabeads ® GC Combo
10 tests
50 tests
10 tests
50 tests
730.01
730.11
730.02
730.12
Dynabeads ® anti-Legionella 100 tests 730.03
NEW
Dynal L10 Tubes 5 tubes 740.03
Dynal Spot On 100 slides 740.04
BeadRetriever TM 1 unit 159.50
BeadRetriever TM Tubes & Tips 240 tests 159.51
BeadRetriever TM Tube Rack 1 unit 159.52
Microbiology
10

MIC RELI TM
Fig. 1.
AutoRELI
– Automated
reverse line
blot
Fig. 2.
Principle of
the RELI
method
Fig. 3.
Position of
probes on
membrane
Fig. 5: Diagrammatic representation of the
principle of the MIC RELI assay.
IC
Ordering Information
MIC RELI TM
Product Volume Product No.
MIC RELI TM 4 Meningococcus 50 tests 750.01
Strip Detection Reagent Kits
A
C
X
Z/29E
IC - Internal PCR inhibition
and hybridisation control
Fig. 4.
Results of MIC RELI typing for eight
meningococcal isolates.
1 - Serogroup A
2 – Serogroup X
3 – Serogroup C
4 – Serogroup W135
5 – Serogroup C
Ref
CtrA
B
W135
6 – Serogroup Y
7 – Serogroup B
8 – Serogroup B
9 – No Template Control
10 – No Amplification Control
Y
NEW
112
New product
Dynal MIC RELI is a new product for Dynal Microbiology. It is a molecular test
that utilises a generic PCR amplification with biotinylated primers that flank
the specific DNA target region of interest. This is followed by hybridisation
of the resulting amplicon to an array of Sequence Specific Oligonucleotide
probes immobilised on a membrane. The immobilisation of these probes
is referred to as a Reverse Line blot and thus gives rise to the acronym
“RELI”.
Under appropriate wash conditions, the probes remain bound only to their
complementary sequences in the amplified DNA. The sensitivity of the system
enables single nucleotide differences to be distinguished. The presence of
the amplicon/probe complex is detected using a simple colorimetric reaction
and appears as a blue precipitate (fig. 5, section 2).
The first product in this range is MIC RELI 4 Meningococcus (fig. 5, section
3). This kit provides a presence/absence test for Neisseria meningitidis and
identification of the common serogroups (fig. 5, section 4). The assay can
be performed manually or can be automated using the Dynal AutoRELI 48
instrument (fig. 5, section 1).
50 tests
250 tests
1000 tests
802.01
802.250
802.999
AutoRELI 48 1 unit 899.05
www.dynalbiotech.com