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Immobilized Metal Ion Interaction 29
have been described (16,28). This is useful in screening different types
of immobilized metal ion-protein interaction variables. These vari-
ables include the selection of appropriate immobilized metal ion type
(16), the effects of temperature and mobile phase conditions (l&,26),
and free metal ions (7) on protein-immobilized metal ion interaction
capacity and affinity.
10. Immobilized metal ions may be useful for the reversible site- or domain-
specific immobilization of functional receptor proteins or enzymes.
Data collected with the estrogen receptor protein suggest that recep
tor immobilization on IDA-Zn(I1) (29) and IDA-Cu(I1) (unpublished)
does not impair receptor function (ligand-binding activity).
Acknowledgment
This work was supported, in part, by the US Department of Agri-
culture, Agricultural Research Service Agreement No. 58-6250-l-003.
The contents of this publication do not necessarily reflect the views or
policies of the US Department of Agriculture, nor does mention of
trade names, commercial products, or organizations imply endorse-
ment by the US Government.
References
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