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46 Hageman and Kuehn
Matrix
Cellulose
Agarose
4s arose
Agarose
Polyacrylamide
Methaclylate
Methacrylate
Silica
Silica
Table 2
Commercial Sources of Benzeneboronate Matrices
Trade name Commercial sources
Indian PhenylBoronate Chermcal Dynamics Corp.,
(PB 0.8) Plainfield, NJ, USA
Matrex Gel (PBA-10, Amicon Corp.,
PGA-30, PBA-60) Danvers, MA, USA
Glyco Gel B Pierce Chemical,
Rockford, IL, USA
Glyc-AlIin Isolab Inc.,
Akron, OH, USA
At&Gel 601 BieRad Laboratones,
(P-6 acrylamide) Richmond, CA, USA
Boric Acid Gel Aldrich Chemical,
(0.1-0.4 mm) Milwaukee, WI, USA
Boric Acid Gel Sigma Chemical, Inc.,
(0.1-0.4 mm) St. Lotus, MO, USA
SelechSpher- Pierce Chemical,
1Om Boronate Rockford, IL, USA
Progel-TSK BoronatedPW Supelco, Inc.,
(prepacked column) Bellefonte, PA, USA
in chromatography (19). Workers wishing more detailed comparisons
of advantages and disadvantages of various boronate matrices may
consult Note 9., and workers wishing to prepare specific types of
boronate matrices are directed to the references given in Table 1. In
contrast to what has appeared in the literature, commercial sources of
boronates universally use 3-aminobenzeneboronic acid to link to solid
supports; nevertheless, five types of matrices can be purchased, and
among these, different chemistry has been used to effect coupling of
the 3-aminobenzeneboronic add to the matrices. Table 2 lists com-
mercial sources for benzeneboronate matrices. The advantages and
disadvantages of various boronate matrices that have been made have
not been formally reviewed. Therefore, a brief comment concerning
each type of matrix will be helpful to investigators contemplating their
use for the first time.
Virtually every support matrix available for general chromatogra-
phy has been derivatized with boronate ligands by a variety of cou-
pling procedures. Table 1 summarizes most of the types of matrices
that have been exploited. These include cellulose, styrene, polyacryl-