MICROPROPAGATION OF GINGER - ETD - University of ...

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Table 2 : Effect of surface disinfectants on per cent contamination and number of
healthy cultured established in ginger explants
Treatments
1 Sodium hypochlorite
(0.5%)
2 Sodium hypochlorite
(0.5%)
Exposure
time (min)
No. of
explants
inoculated
No. of
explants
contami
nated
10 30 19
(63)
15 30 12
(40)
No. of
healthy
cultures
established
11 (37)
18 (60)
3 HgCl2 (0.1%) 10 30 9 (30) 21
(70)
4 HgCl2 (0.1%) 12 30 3
(10)
5 HgCl2 (0.1%) 15 30 9
(24)
27
(90)
14
(43)
S.Em+ - - 0.4 0.7
CD at 1% - - 1.4 2.5
Values in the parenthesis indicate percentage
recorded in control and minimum (1.3 cm) was recorded in 1.0 mg/l NAA in axillary bud
explant.
4.5 IDENTIFICATION OF SUITABLE HARDENING MEDIUM
FOR MICROPROPAGATED GINGER PLANTLETS
4.4.1 Survival percentage of plantlets
The data regarding the survival percentage of plantlets during hardening at different
intervals are presented in the Table 7.
Significant differences were noticed for survival percentage of plantlets after 15 and
30 days after transferring to hardening media. The maximum survival was noticed on peat
medium with 80 per cent at 15 and 30 days after transfer on to hardening medium. However,
the lowest survival was on a sand showing 65 and 50 per cent at 15 and 30 days after
transfer to hardening medium respectively.
4.5.2 Height of the plantlets
Significant differences were observed among the treatments. The maximum height
was recorded in peat (5 cm) which was significantly superior to all other treatments. This was
followed by vermiculite (4.1 cm) results are presented in Table 8.