MICROPROPAGATION OF GINGER - ETD - University of ...

VI. SUMMARY
The present study on micropropagation of Zingiber officinale Rosc. was conducted in
the tissue culture laboratory in the Department of Horticulture, University of Agricultural
Sciences, Dharwad during 2004-06.
Ginger is an important spice crop grown in India. It is herbaceous rhizomatous
perennial plant. The method of propagation is through sections of underground rhizomes.
However, it has a dormancy period and sproutes only during the monsoon that to only 5 to 6
plants can be obtained from one single rhizome per year. To overcome this, micropropagation
may plays a important role in rapid mass propagation of ginger. Plants produced through
micropropagation are true to type and are free from diseases. The present investigations
were carried out to standardize surface sterilization of explants, suitable explant type for
culture establishment, growth regulators for shoot multiplication and rooting and to evaluate
suitable hardening media.
Of the various concentrations of HgCl2 and NaOCl tried for surface disinfection.
Among that the different concentration of HgCl2 tried at 0.1 per cent emerged as the best
treatment.
Different types of the explants viz., shoot tip, axillary bud, root tip were tried. It was
observed that shoot tip gave the best results and emerged as suitable explant for ginger
culture establishment.
Among different concentrations of cytokinins viz., BAP and kinetin. 2.0 mg/l BAP gave
the highest number of multiple shoots.
Among different auxins tried at different concentrations for rooting of microshoots, MS
medium with 1 mg/l IBA gave the highest number of roots.
Three different media viz., peat, vermiculite and sand were tried for hardening of
plantlets. Peat gave the maximum survival percentage with better plant growth resulting as a
suitable medium for hardening.