EuroChrom 2000 for Windows Basic Edition version 2.05

Injection
Unretained Peak
t a
Time
t
1
t
2
W 1
Separation of Two Chromatographic Components
Note: To accurately calculate suitability values, the sampling frequency (set in Acquisition Setup) must be
set to provide at least 20 data (10 for the leading edge and 10 for the tailing edge of the peak) points
for the narrowest peak of interest.
Relative Retention (Selectivity)
t 2 - ta
α =
t 1 - ta
Where
α = Relative retention.
t2 = The retention time measured from point of injection
Capacity Factor (k’)
Where
t 2
k' = −1
ta
ta = The retention time of an inert component not retained by the column, taken
from "Unretained R(etention) T(ime)" in the Detection Parameters section.
t1 = The retention time from point of injection for reference peak defined in the
Detection Parameters section. If no reference peak is found, this value
becomes -1.
k' = Capacity Factor
t 2 = The retention time measured from point of injection
t a = The retention time of an inert component not retained by the column, taken
from "Unretained R(etention) T(ime)" in the Detection Parameters section.
USP (United States Pharmacopia) calculation method
Theoretical Plates
t
n = 16( )
W
2
Where
n = theoretical plates
t = The retention time of the component
W = The width of the base of the component peak using tangent method.
W 2