EuroChrom 2000 for Windows Basic Edition version 2.05

Peak Asymmetry (Tailing Factor)
.
T = W 0 05
2f
Where
T = Peak asymmetry, or tailing factor
W0.05 = The distance from the leading edge to the tailing edge of the peak, measured
at a point 5% of the peak height from the baseline
f = The distance from the peak maximum to the leading edge of the peak at the
position of 5% peak height
Peak Front Peak Tail
f
W 0.05
Peak Maximum
Asymmetric Peak
0.05 h
Resolution
2( t 2−t1) R =
W2 + W1
Where
R = Resolution between a peak of interest (peak 2) and the peak preceding it
(peak 1).
t2 = The retention time measured from point of injection of peak 2.
t 1 = The retention time measured from point of injection of peak 1.
W 2 = The width of the base of the component peak 2
W 1 = The width of the base of the component peak 1
EMG (Exponential Modified Gaussian) calculation method
Theoretical Plates
Where
N =
� t �
�
W
�
� 0.
1�
41.
7 ×
b0.
1
+ 1.
25
a0.
1
2
N= The number of theoretical plates
t= The retention time of the component
W0.1 = The width of the peak at the position of 10% peak height
a0.1 = The width of the first half (start to top) of peak at the position of 10% peak
height
b0.1 = The width of the second half (top to end) of peak at the position of 10% of
peak height
Peak Asymmetry (Tailing Factor)
.
T = W 0 05
2f
Where
h