PROBIOTICS WATCH - Probiotics In Practice

A scientific tool supported by dAnone 

PROBIOTICS WATCH 

Contents : 

EDITORIAL 

THE MAIN POINTS OF 

THE qUARTER 

1. CHARACTERISATION OF 

PROBIOTICS AND 

PROBIOTIC FOODS 

2. PHYSIOLOGICAL 

INTERACTION WITH THE 

HOST 

3. IN VIVO OUTCOMES OF 

PROBIOTIC INTAKE 

REFERENCES 

KEY DATES 

NOTEWORTHY PUBLICATIONS 

Manufacturing process modifies L. rhamnosus original in vitro properties 

intestinal mucosa responses to probiotics differ from one strain to another: 

results of transcriptome analysis 

intake of probiotics seems to prevent nec in preterm infants 

Issue N°4 

Jan. 2011 

This probiotics watch is designed as a time-saving tool for scientists and clinicians interested in 

probiotic research. In an interactive format, the quarterly report provides timely, quasi-exhaustive 

lists of the scientific publications of the previous three months. It sorts them by topic and highlights 

some of the most relevant results. Readers can also check out upcoming scientific events and 

regular bibliometric analyses. 

Objectivity is a strong commitment, that’s why the articles are selected by an editorial committee, 

composed of renowned scientists in the field. Editorial committee members also comment on 

what they believe are the quarter’s most relevant publications. 

Last but not least each issue features an editorial by a probiotic expert, which offers special insight 

into this fascinating field of science. 

Method 

EDITORIAL 

A THOUGHT FOR FOOD: TIME HEALTH CLAIM 

STUDIES HAD OWN METHODOLOGY? 

by Pr. Francisco Guarner 

Hospital Vall d’Hebron, Barcelona, Spain 

« Inappropriate food habits rank on the top of the list of causes 

of disease and early death in developed societies. Obesity, type II 

diabetes, coronary heart disease and cancer, among others, are 

obvious examples of modern world epidemics ... » 

intestinal lactobacilli protects children from developping allergies: a species-dependent effect 

Multispecies probiotic intervention alleviates bacterial alterations in ibs patients 

L. rhamnosus GG relieves symptoms of abdominal pain in ibs children 

A mechanism explaining the preventive effect of probiotics against colitis 

L. reuteri consumption improves colic symptoms in breastfed newborns 

control of mothers’ diet during pregnancy benefits infants metabolic health 

> read more 

Mothers’ weight or weight gain during pregnancy impacts the intestinal flora composition of the infants 

> Monitoring period : 28/07/2010 to 15/11/2010 > Database : Medline > Result : 371 publications 

> Keywords : probiotic / lactic acid bacteria / streptococcus thermophilus / lactobacilli / fermented milk / bifidobacteria 

1

A SCIENTIFIC TOOL SUPPORTED BY DANONE 

PROBIOTICS WATCH 

Contents : 

EDITORIAL 


THE QUARTER 

1. CHARACTERISATION OF OF 





HOST 



REFERENCES 

KEY KEY DATES > Home page 

A THOUGHT FOR FOOD: TIME HEALTH CLAIM 

STUDIES HAD OWN METHODOLOGY? 

by Pr. Francisco Guarner 

Hospital Vall d’Hebron, 

Barcelona, Spain. 

More info about health claims related to gut and immune function: 

http://www.efsa.europa.eu/fr/ 

p p 

events/event/nda101202.htm events/event/nda101202.htm http://www.gut-health.eu/ 

p g 

Issue N°4 

Jan. 2011 

Inappropriate food habits rank on the top of the list of causes of 

disease and early death in developed societies. Obesity, type II 

diabetes, coronary heart disease and cancer, among others, are 

obvious examples of modern world epidemics. These pathological 

conditions of high prevalence are all certainly associated with bad 

food habits. There is no reason that this pernicious trend should 

not be amenable to shift towards a healthier course, provided 

that knowledge about the risks and benefits associated with food 

consumption are satisfactorily transmitted to the population. One 

simple way of doing this, among many others, is the intelligent use 

of the information included in food product labelling. A few years 

ago, a Regulation of the European Parliament and of the Council of 

the European Union on nutrition and health claims made on foods 

was promulgated (EC 1924/2006). The regulation states that health 

claims for food labelling must be based on scientific evidence. 

In order to ensure that the claims made are truthful and can 

be understood by consumers, they must undergo a specific 

assessment and authorization procedure involving consultation 

with the European Food Safety Authority. 

Researchers have been developing novel methodologies aimed at proper substantiation of healthy effects of foods other than their 

inherent nutritional value. As compared with studies on pharmaceuticals, particular challenges for the investigation of health benefits 

of foods are the length of the studies and the need for adequate control products. Drugs may induce rapid-onset effects but, as 

shown by observational studies, foods usually have a strongly defined impact on health after long-term exposure as opposed to nonexposure. 

Intervention studies carried out over a long period of time may have intrinsic difficulties in terms of compliance and drop-out 

of participants during the follow-up. Likewise, drugs studies are controlled by a pharmacologically inert placebo matrix supplied in pills, 

which are indistinguishable from the pills containing the active drug. This provision is not always fully suitable within the design framework 

of a prospective intervention study evaluating food. 

Unfortunately, recent opinions cast by the EFSA-NDA panel after the assessment of beneficial health effects of probiotics raise doubts 

about whether such differences are being perceived by the experts in the panel. Ignoring the outcome of long-term studies on the basis 

of drop-out rates and disputing the validity of ‘per protocol analysis’ makes little sense for the progression of the field. As opposed to 

medicines, it is probably more relevant to identify long-term effects in subjects with confirmed exposure to the tested food product (per 

protocol population), rather than applying statistical criteria validated for short term studies on acute effects. Likewise, long term studies 

are less susceptible to influences from potential confounding factors such as diet or concomitant drug intake, as these factors tend to be 

stable over the long term. In other words, such issues may be very relevant in short term studies, but not necessarily critical in long term 

studies, and should not be used as criteria for rejecting the information obtained in well-designed and conducted studies. However, these 

reasons were claimed by the panel in order to reject an application of a specific mixture of probiotic strains that was tested in two longterm 

studies (5 and 6 months) showing significant reduction of scores of abdominal discomfort. 

Another interesting point is that study designs for interventions with food products should be able to compare ‘apples and pears’, without 

claiming violation of the ‘double-blind’ rule for randomized controlled intervention trials. The critical issue here is to ensure that participants 

do not know whether they are in the active or control arm (even if products are fully distinguishable), and investigators analyzing samples 

and data deal only with coded information. This may be a difficult matter in some settings but it is clearly achievable. Our minds should 

be open to identifying valid procedures for the adequate investigation of food effects on health. It is unfortunate that disputing the validity 

of the ‘double blind’ design of a randomized controlled trial testing a probiotic strain in fermented milk as compared with acidified milk 

was the reason given by the panel for rejecting the study. This particular study was peer-reviewed, accepted, and published a few years 

ago in the British Journal of Medicine; a highly competitive scientific forum. The simplistic approach of ignoring outcomes and rejecting 

procedures that do not fulfil ad integrum methodologies validated for drug studies is not the best way to evaluate research on food. 

Moreover, the negative approach is likely to compromise future developments of this interesting field of nutritional science. 

The legacy of these NDA-panel opinions will be the favour of nutritional supplements in the form of pills or sachets, as they share physical 

characteristics with drugs. These supplements are optimal for conducting research studies that are fully compliant with the criteria being 

applied by the panel. Eventually, supplements may end up flooding our “food” market. However, this was not the spirit or aim of the EC 

regulation, and it is certainly not what consumers need and expect from the food sciences or marketplace. 

2

PROBIOTICS 

WATCH 

ISSUE N°4 THE MAIN POINTS OF THE QUARTER 

Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Adults 

The elderly 

Children 

A quarter with many in vitro studies 

Meta-analysis 0,7% 

Clinical 

19,9% 

Animal 

26,7% 

In vitro 

52,7% 

371 publications were retrieved from Medline for the last quarter of 2010, 

including 25% of reviews. This survey period (like the preceding ones) is 

characterised by the publication of a majority of in vitro studies and a higher 

number of reviews than in the preceding quarters. 

Probiotics in clinical trials are mainly assayed against infections 

A total of 55 clinical trials were published in the last quarter; 37 were performed in adults, 1 in the elderly and 17 in 

children. Probiotic intake is mainly assayed against infections (gastro-intestinal, respiratory, genito-urinary, etc.) in 

adults and children. 

IN VIVO OUTCOMES OF 

PROBIOTICS INTAKE 

PHYSIOLOGICAL 

INTERACTION 

WITH THE HOST 

DISTRIBUTION OF CLINICAL STUDIES ACCORDING TO THEMATIC FIELD 

Clinical trials are mainly performed with Lactobacillus 

Clinical trials focused on the health effects of probiotics were mainly performed with Lactobacillus (74.5% of the 

studies). These trials mainly investigated the effects of three species of probiotics: Lactobacillus rhamnosus (21.8% 

of the studies, half of which were related to the strain L. rhamnosus GG), L. acidophilus (16.4%), and Bifidobacterium 

breve (12.7%). 

L. rhamnosus 

L. acidophilus 

L. plantarum 

L. casei 

L. paracasei 

L. helveticus 

B. breve 

B. lactis 

B. longum 

S. thermophilus 

Nutrition: obesity, cholesterol, etc. 

IBD & other inflammatory diseases 

Effects of probiotics on immunity 

Mucosa response to probiotics 

NUMBERS OF QUOTATIONS (IN % OF TOTAL CLINICAL STUDIES) 

3,6 

3,6 

3,6 

5,5 

IBS & functional GI disorders 

Allergy & atopy 

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 

Numbers of papers 

7,3 

Other physiological effects 

Effects of probiotics on microbiota 

9,1 

9,1 

12,7 

16,4 

Infections 

21,8 

Others 

21,8% 

Bifidobacteria 

34,5% 

0 5 10 15 20 

25 

Numbers of papers 

New applications 

& other 

Lactobacillus 

74,5% 

3



ISSUE N°4 

Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

> The studies performed in humans 

are highlighted using the following 

symbols : 

for the GENERAL POPULATION 

for CHILDREN 

for the ELDERLY 

In VITRO/ANIMALS 

studies are highlighted 

with the symbol : 

1. CHARACTERISATION OF PROBIOTICS AND PROBIOTIC FOODS 

1.1. TECHNOLOGY OF PROBIOTICS AND PROBIOTIC FOODS 

Manufacturing process modifies L. rhamnosus original in vitro 

properties 

OBJECTIVES 

Production and manufacturing methods and the food carrier may influence the properties of probiotic 

strains, and have an impact on the outcome of clinical intervention studies. The aim of the present study 

was to establish whether the properties of Lactobacillus rhamnosus may differ depending on the product 

and source of the strain. 

METHODS & MEASURES 

Fifteen different L. rhamnosus isolates, of which fourteen were labelled as L. rhamnosus GG, were 

isolated from specific probiotic products. The micro-organisms were phenotypically and genotypically 

characterised. Their adhesion properties were compared using the human intestinal mucus model, and 

the ability of the isolates to influence model pathogen adhesion to human colonic mucus was assessed. 

All L. rhamnosus isolates used were confirmed as members of the species L. rhamnosus. 

RESULTS 

Except the reference strain OL, all L. rhamnosus isolates showed randomly-amplified polymorphic DNA, 

enterobacterial repetitive intergenic consensus and pulsed-field gel electrophoresis profiles identical to 

that of L. rhamnosus GG (ATCC 53103). All L. rhamnosus isolates showed similar tolerance to acid and 

were able to bind to human colonic mucus. However, pathogen exclusion by inhibition and competition 

varied significantly among the different L. rhamnosus isolates and pathogens tested. 

CONCLUSION 

The results suggest that different sources of the same probiotic strain may have significantly modified 

strain properties. This should be considered in in vivo studies on human subjects and also for quality 

control of probiotic products. 

Grzeskowiak L, Isolauri E, Salminen S, Gueimonde M. Manufacturing process influences properties of probiotic bacteria. Br J Nutr. 2010 Nov 9:1-8. 

COMMENTARY 

from Bruno Pot, Institut Pasteur de Lille, France 

« In the past, technological properties were considered to be important selection criteria for probiotics, 

because strains need to be grown reproducibly, remain viable in high amounts for longer time periods and 

remain genetically stable. Besides helping to preserve a genetically stable culture, the preservation matrix was 

also shown to influence survival during GIT passage and to determine some aspects of probiotic functionality. 

Interactions with the host have, for example, been shown to depend on the growth phase of the culture 

administered (1) , and several papers have discussed the importance of metabolic compounds in probiotic 

effects (2,3) . 

The authors of this new paper also point out that technological conditions in production and preservation may 

have an impact on the expected outcome of human clinical studies. Although these findings seem logical, they 

are rarely considered in clinical trial setups. While numbers and mode of delivery are always defined, batch 

production history is rarely detailed. 

The authors compared thirteen Lactobacillus rhamnosus GG strains used in clinical intervention studies. All 

strains proved to be identical using RAPD, REP-PCR and PFGE typing, showed similar tolerance to acid 

and were able to adhere to human colonic mucus. In pathogen exclusion experiments, however, inhibition 

and competition of pathogens varied significantly. These indings suggested that different sources of the 

same probiotic strain may result in varying properties which may help to explain earlier differences in clinical 

outcomes, linked to different production schedules. 

Although this paper gives no explanation for the variations observed, one can easily expect that the results 

obtained may actually be dampened by the fact that all strains were regrown under identical laboratory 

conditions, eliminating some of the physiological differences before being tested. 

In any case, it seems a good idea to consider production history in studies on human subjects and also to 

define suitable quality control measures for probiotic products, as suggested by the authors. » 

1. Van Baarlen P, FJ. Troost, S van Hemert, C van der Meer, W M de Vos, P J de Groot, GJEJ Hooiveld, R-J M Brummer and M 

Kleerebezem. Differential NF-kB pathways inductionby Lactobacillus plantarum in the duodenum of healthy humans correlating with 

immune tolerance. 2009 roc Natl Acad Sci USA doi:10.1073/pnas.0809919106. 

2. Sokol Harry, Benedicte Pigneur, Laurie Watterlot, Omar Lakhdari, Luis G. Bermu-dez-Humaran, Jean-Jacques Gratadoux, Sebastien 

Blugeon, Chantal Bridonneau, Jean-Pierre Furet, Gerard Corthier, Corinne Grangette, Nadia Vasquez, Philippe Pochart, Germain 

Trugnan, Ginette Thomas, Herve M. Blottiere, Joël Doré, Philippe Marteau, Philippe Seksik, Philippe Langella. Faecalibacterium 

prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. 2008. Proc 

Natl Acad Sci USA 105:16731–16736. 

3. Heuvelin Elise, Lebreton Corinne, Grangette Corinne, Pot Bruno, Cerf-Bensussan Nadine, Heyman Martine. 

Mechanisms involved in alleviation of inflammation by Bifidobacterium breve soluble factors (2009). PlosOne 4(4), e5184. 

4



ISSUE N°4 

Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

2. PHYSIOLOGIAL INTERACTION WITH THE HOST 

2.1. MUCOSA RESPONSE TO PROBIOTICS (TROPHYCITY & STRUCTURE) 

Intestinal mucosa responses to probiotics differ from one strain to 

another: results of transcriptome analysis 

OBJECTIVES/BACKGROUND 

Probiotic bacteria are proposed to deliver health benefits to the consumer by modulation of intestinal 

function through largely unknown molecular mechanisms. The aim of this study is to explore in vivo 

mucosal responses of healthy adults to probiotics via analyses of duodenal transcriptomes. 

DESIGN 

Randomized, placebo-controlled, cross-over study. 

SETTINGS & PARTICIPANTS 

Seven healthy, nonsmoking volunteers (aged 24±4 years), with no history of gastrointestinal symptoms 

and free of any form of medication, were investigated on four separate occasions: 3 bacterial interventions 

each with one of the 3 strains (Lactobacillus acidophilus Lafti-L10, L. casei CRL-431, and L. rhamnosus 

GG) and one placebo. Interventions were separated by a 2 week wash-out period. Volunteers fasted 

overnight and were administered a maltodextrin solution at the start of the intervention, after which 

they were provided every 30 min with a preparation containing reconstituted freeze-dried bacteria 

resuspended in maltodextrin solution or a preparation only containing the maltodextrin solution (placebo) 

for a period of 6 h. Tissue samples were obtained from the horizontal part of the duodenum. Total RNA 

extracted from these biopsies was hybridized to Affymetrix whole genome expression arrays. 

MAIN OUTCOME 

Analysis of the transcriptome of duodenal biopsies. 

RESULTS 

In the mucosa of the proximal small intestine of healthy volunteers, each strain of the probiotics induced 

differential gene-regulatory networks and pathways in the human mucosa. Comparison of these specific 

expression profiles with response profiles associated with pharmaceutical and other biologically-active 

compounds showed that the responses to probiotic strains correlated significantly with responses to 

compounds active in the regulation of immune responses, the cell cycle, blood pressure, and water and 

ion homeostasis. Mucosal responses to L. acidophilus mainly involve regulation of immune response, 

hormonal regulation of tissue growth and development, and ion homeostasis, those for L. casei involve 

proliferation, Th1/Th2 balance, and hormonal regulation of blood pressure and those for L. rhamnosus 

involve wound healing, IFN response, and ion homeostasis. Transcriptomes clustered per person, not 

per intervention, show that person-to-person variation in gene expression was the largest determinant 

of differences between transcriptomes. Nevertheless, the consumption of different probiotic lactobacilli 

led to markedly different expression profiles in vivo in human mucosa. 

CONCLUSION 

This study elucidates how the intestinal mucosa of healthy humans perceives different probiotics and 

anticipates that responsiveness to probiotics is not only determined by the characteristics of the bacterial 

strain consumed but also by genetic background, resident microbiota, diet, and lifestyle. This study 

could, therefore, be among the first steps to investigate the interplay between microbiota, probiotic, or 

other nutritional supplements and human genetics towards personalized nutrition. 

van Baarlen P, Troost F, van der Meer C, Hooiveld G, Boekschoten M, Brummer RJ, Kleerebezem M. Microbes and Health Sackler Colloquium: 

Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways. 

Proc Natl Acad Sci U S A. 2010 Sep 7. 

COMMENTARY 

from James Versalovic, Baylor College of Medicine, Houston US 

« This study (van Baarlen et al.) described the differential effects of three different Lactobacillus species on cell 

signaling pathways in the duodenum of healthy adults. The study included three commonly used commercial 

Lactobacillus strains, and the study was designed as a randomized, placebo-controlled crossover study 

(7 human adults with 4 interventions each). Each individual consumed Lactobacillus acidophilus (5,2 x 10 10 

organisms), Lactobacillus casei (3.2 x 10 10 organisms), Lactobacillus rhamnosus (1.68 x 10 10 organisms), or 

a control maltodextrin solution during a 6-hour period prior to collection of total human RNA from duodenal 

biopsies. Consumption of L. acidophilus induced the expression of immune response genes, genes involved 

in hormonal regulation of tissue growth and development, and genes involved in water and ion homeostasis. 

Consumption of L. casei resulted in enhanced expression of genes involved in the regulation of cell proliferation, 

balance of Th1/Th2 immune responses, metabolism, and hormonal activity involved in blood pressure 

regulation. Consumption of L. rhamnosus resulted in differential gene expression in pathways involved in 

wound repair and healing, angiogenesis, interferon responses, calcium signaling, and ion homeostasis. This 

ground-breaking study describes the detailed differences of short-term (acute) effects by probiotics on human 

signaling pathways (administration time of 6 hours with 2-week long wash-out periods). The results point the 

way towards targeted use of probiotics for specific applications in human nutrition and medicine. » 

5



ISSUE N°4 2. PHYSIOLOGICAL INTERACTION WITH THE HOST 

Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

2.2. EFFECTS OF PROBIOTICS ON MICROBIOTA 

Probiotic and nutrient intakes generate specific metabolite 

procuction in mice 

OBJECTIVES 

The complex association of gut flora collectively extends the processing of undigested food to the benefit 

of the host through metabolic capacities not encoded in mammalian genomes. At present, metabolic 

insights into gut microbiota metabolism remain limited due to the inaccessibility of the intestinal habitat 

and gut microbiota complexity. Over the past few decades, the practice of metabonomics has evolved 

from diagnosis and identification of biomarkers for a clinical condition towards deciphering the metabolic 

predisposition and responses to different individual dietary modulations. The present work monitored 

faecal metabolic changes during microbial establishment collected from various groups of mice 

supplemented with probiotics or nutrients. 


1 H-NMR-based metabolic profiling of mouse feces was carried out over time in different microbiome 

mouse models, including conventional (n=9), conventionalized (n=10), and “humanized” gnotobiotic 

mice inoculated with a model of human baby microbiota (HBM, n=17). HBM mice were supplemented 

with Lactobacillus paracasei with (n=10) and without (n=7) prebiotics. Animals not supplemented with 

prebiotics received a diet enriched in glucose and lactose as placebo. 

RESULTS 

In conventionalized animals, microbial populations and activities converged in terms of multivariate 

mapping toward conventional mice. Both groups decreased bacterial processing of dietary proteins 

when switching to a diet enriched in glucose and lactose, as described with low levels of 5-aminovalerate, 

acetate, and propionate and high levels of lysine and arginine. The HBM model differs from conventional 

and conventionalized microbiota in terms of type, proportion, and metabolic activity of gut bacteria (lower 

short chain fatty acids (SCFAs), lactate, 5-aminovalerate, and oligosaccharides, higher bile acids and 

choline). The probiotic supplementation of HBM mice was associated with a specific amino acid pattern 

that can be linked to L. paracasei proteolytic activities. The combination of L. paracasei with galactosyloligosaccharide 

prebiotics was related to the enhanced growth of bifidobacteria and lactobacilli, and a 

specific metabolism of carbohydrates, proteins, and SCFAs. 

CONCLUSION 

The present study describes how the assessment of metabolic changes in feces may provide information 

for studying nutrient-microbiota relationships in different microbiome mouse models. 

Martin FP, Sprenger N, Montoliu I, Rezzi S, Kochhar S, Nicholson JK. Dietary modulation of gut functional ecology studied by faecal metabonomics. 

J Proteome Res. 2010 Oct 1;9(10):5284-95. 

6



ISSUE N°4 

Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

3. IN VIVO OUTCOMES OF PROBIOTIC INTAKE 

3.1. INFECTIONS (GI, RESPIRATORY INCL. FLU, GENITO-URINARY, OTHER) 

Intake of probiotics seems to prevent NEC in preterm infants 

OBJECTIVES / BACKGROUND 

The objective was to assess whether the combined use of probiotics may prevent the occurrence of 

necrotizing enterocolitis (NEC) stage ≥2, by Bell’s criteria, in preterm infants. 

DESIGN 

A double-blind, randomized, controlled clinical trial. 


The trial was conducted in 231 preterm infants weighing from 750 to 1499 g at birth. The intervention group 

was composed of 119 infants who received human milk with probiotic supplementation (Bifidobacterium 

breve and Lactobacillus casei) and a control group of 112 infants who received human milk containing no 

probiotics. The intervention was initiated on the second day of life of all infants and was maintained until 

the 30th day of life, a diagnosis of NEC, discharge from the hospital, or death, whichever occurred first. 

MAIN OUTCOME 

The primary outcome was the occurrence of NEC stage ≥2 as defined by Bell’s modified criteria. 

RESULTS 

Four confirmed cases of NEC (Bell’s stage ≥2) were observed in the control group and none in the 

probiotic group. Although no differences were observed between the groups in relation to the beginning 

of enteral feeding and advancements for enteral feeding volume, infants in the probiotics group achieved 

full enteral feeding faster than did those in the control group (P=0.02). The survival curve, determined 

with the Kaplan-Meier method for evaluating the complete transition time from orogastric feeding tube 

to breastfeeding, showed a shorter time in the probiotics group (P=0.03). 

CONCLUSION 

Oral supplementation of B. breve and L. casei reduced the occurrence of NEC (Bell’s stage ≥2). As this 

study considers the transition time from orogastric tube feeding to breastfeeding as a further indicator of 

the maturation of digestive tract motor function in newborns, it was concluded that an improvement in 

intestinal motility might have contributed to this result. 

Braga TD, da Silva GA, de Lira PI, Carvalho Lima M. Efficacy of Bifidobacterium breve and Lactobacillus casei oral supplementation on necrotizing 

enterocolitis in very-low-birth-weight preterm infants: a double-blind, randomized, controlled trial. Am J Clin Nutr. 2010 Oct 27. 

COMMENTARY 

from Hania Szajewska, The Medical University of Warsaw, Poland 

« Necrotizing enterocolitis (NEC) is one of the most serious, life-threatening gastrointestinal diseases, with 

a high mortality rate. The significance of the problem of NEC justifies any attempt to prevent this condition. 

A recent, updated meta-analysis by Deshpande et al. (1) (search date: March 2009) identified 11 randomized 

controlled trials (RCTs)(n=2176) and concluded that there are significant benefits of probiotic supplementation 

in reducing the risk of NEC (RR 0.35, 95% CI 0.23 to 0.55) and all-cause mortality (RR 0.42, 95% CI 0.29 to 

0.62) in preterm neonates. The authors also concluded that ‘probiotics should be now offered as a routine 

(2) (3) 

therapy for preterm infants’. This opinion is not universally shared, including by some scientific societies. 

One of the reservations is that it is still not clear which products should be administered, at what dosage, and 

for how long. In this respect, the study by Braga et al. that evaluated probiotics not yet investigated for the 

prevention of NEC is of interest. In line with previous studies, the risk of NEC was significantly lower in the 

probiotic group (although this was of borderline statistical significance). In contrast to many earlier studies, the 

risk of death did not differ significantly between the probiotic and control groups. 

Demonstrating the efficacy of the combination of B. breve & L. casei given to preterm infants for the prevention 

of NEC through a well-conducted RCT would be a praiseworthy goal. Such evidence would be appreciated 

in settings where this probiotic combination is available. Unfortunately, there is one major limitation to the 

interpretation of this study. The calculated sample size was 564 infants. However, the study was interrupted by 

the External Study Committee one year after the intervention began when they observed ‘a major benefit in one 

of the groups’. At this time, there were 231 participants. In my opinion, the results demonstrating prevention 

of NEC were not yet so striking as to interrupt the study. Similar views were expressed by the authors of the 

accompanying editorial. (4) This criticism does not invalidate the study; it merely indicates that, as so often is 

the case, further studies are needed before the effectiveness of the combination of B. breve & L. casei given to 

preterm infants for the prevention of NEC is established. » 

1. Deshpande G, Rao S, Patole S, Bulsara M. Updated meta-analysis of probiotics for preventing necrotizing enterocolitis in preterm 

neonates. Pediatrics 2010;125:921-30. 

2. ESPGHAN Committee on Nutrition: Agostoni C, Buonocore G, Carnielli VP, De Curtis M, Darmaun D, Decsi T, Domellöf M, Embleton 

ND, Fusch C, Genzel-Boroviczeny O, Goulet O, Kalhan SC, Kolacek S, Koletzko B, Lapillonne A, Mihatsch W, Moreno L, Neu J, 

Poindexter B, Puntis J, Putet G, Rigo J, Riskin A, Salle B, Sauer P, Shamir R, Szajewska H,Thureen P, Turck D, van Goudoever JB, Ziegler 

EE; ESPGHAN Committee on Nutrition. Enteral nutrient supply for preterm infants: commentary from the European Society of Paediatric 

Gastroenterology, Hepatology and Nutrition Committee on Nutrition. J Pediatr Gastroenterol Nutr 2010;50:85-91. 

3. Thomas DW, Greer FR; American Academy of Pediatrics Committee on Nutrition; American Academy of Pediatrics 

Section on Gastroenterology, Hepatology, and Nutrition. Probiotics and prebiotics in pediatrics. Pediatrics 2010;126:1217-31. 

4. Rushing J, Neu J. Probiotics for pregnant women and preterm neonates. Am J Clin Nutr 2011;93:3-4. 

7



ISSUE N°4 3. IN VIVO OUTCOMES OF PROBIOTIC INTAKE 

Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

An efficacious bifidobacterium against respiratory infections in infants 


The impact of the administration of Bifidobacterium animalis subsp. lactis BB-12 (BB-12) on the risk of 

acute infectious diseases was studied in healthy infants. 

DESIGN 

Randomized, double-blind, placebo-controlled trial. 


109 infants (1 month old) were assigned to a probiotics group receiving a tablet containing BB-12 (n=55) 

or to a control group receiving a control tablet (n=54). Tablets were administered from the age of 1-2 

months to 8 months with a slow-release dummy or a spoon. Breastfeeding habits, dummy use, dietary 

habits, medication and all signs and symptoms of acute infections were registered. At the age of 8 

months, faecal samples were collected for BB-12 determination. 

MAIN OUTCOME 

The primary outcome measures were the cumulative reported incidence of acute respiratory infections 

and doctor-diagnosed acute otitis media occurring before the age of 8 months. Successful intestinal 

passage of BB-12 was chosen as the secondary outcome measure. 

RESULTS 

The baseline characteristics of the two groups were similar, as was the duration of exclusive breastfeeding. 

BB-12 was recovered in the faeces of 62% of the infants receiving the BB-12 tablets. The daily duration of 

dummy sucking was not associated with the occurrence of acute otitis media. No significant differences 

between the groups were observed in reported gastrointestinal symptoms, otitis media or use of 

antibiotics. However, the infants receiving BB-12 were reported to have experienced fewer respiratory 

infections (65 vs. 94 %; RR 0·69; 95% CI 0·53, 0·89; P=0.014) than the control infants. 

CONCLUSION 

Controlled administration of B. lactis BB-12 in early childhood may reduce respiratory infections. The 

children participating in the present study were remarkably healthy, apart from respiratory-infections. 

Thus, for the other infectious and non-infectious diseases, the number of infants should have been 

higher to be able to detect any differences between the groups. With regard to these diseases, the 

present study did not even detect a trend for differences between the groups. 

Taipale T, Pienihäkkinen K, Isolauri E, Larsen C, Brockmann E, Alanen P, Jokela J, Söderling E. Bifidobacterium animalis subsp. lactis BB-12 in 

reducing the risk of infections in infancy. Br J Nutr. 2010 Sep 24:1-7. 

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KEY DATES 

Symbiotic in preventing morbidities in young children 


This study reports a large randomized controlled trial, evaluating effect of providing combination of 

prebiotic and probiotic in milk for one year on both gut and non-gut related illnesses among children in 

a community based setting in India. 

DESIGN 

Randomized, double-blind, controlled trial. 


Children 1-3 years of age were randomly allocated to receive either control milk (n=312) or the same 

milk fortified with prebiotic oligosaccharide and Bifidobacterium lactis HN019 (n=312). The intervention 

was carried out for 1 year. Biweekly household surveillance was conducted to gather information on 

compliance and morbidity. 

MAIN OUTCOME 

Primary outcomes were not explicitly prespecified in the protocol; the intent was to evaluate impact on 

common childhood illnesses including diarrhoea, pneumonia, and febrile illness. Diarrhoea was defined 

as ≥3 loose or watery stools in 24 hours, and diarrhoeal episodes were considered recovered on first day 

of 3 diarrhoea free days. 

RESULTS 

Overall, there was no effect of prebiotic and probiotic on diarrhoea (6% reduction, 95% CI: -1 to 12%; 

P=0.08). Incidence of dysentery episodes was reduced by 21% (95% CI: 0 to 38%; P=0.05). Incidence 

of pneumonia was reduced by 24% (95% CI: 0 to 42%; P=0.05) and severe acute lower respiratory 

infection by 35% (95% CI: 0 to 58%; P=0.05). Compared to control group, children in probiotic/prebiotic 

group had 16% (95% CI: 5 to 26%, P=0.004) and 5% (95% CI: 0 to 10%; P=0.05) reduction in days with 

severe illness and high fever respectively. 

CONCLUSION 

This trial shows that consumption of a symbiotic resulted in a significant reduction of dysentery, 

respiratory morbidity and febrile illness in young children and in a lack of effect against diarrhoea. This 

finding suggests that fortification with prebiotic and probiotic together may provide one of the potential 

interventions to reduce the burden of common childhood morbidities. 

Sazawal S, Dhingra U, Hiremath G, Sarkar A, Dhingra P, Dutta A, Verma P, Menon VP, Black RE. Prebiotic and probiotic fortified milk in prevention 

of morbidities among children: community-based, randomized, double-blind, controlled trial. PLoS One. 2010 Aug 13;5(8):e12164. 

9



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3.2. ALLERGY & ATOPY 

Intestinal lactobacilli protects children from developing allergies: 

a species-dependent effect 


Diminished exposure to harmless micro-organisms, such as lactobacilli, has been suggested to play 

a role in the increased prevalence of allergic disorders in Westernized communities. The development 

of allergies depends on both environmental factors and genetic variations, including polymorphisms 

in genes encoding pattern recognition receptors. The present study examines the effects of both 

colonization with specific Lactobacillus species and genetic variations in DC-SIGN, a pattern recognition 

receptor on dendritic cells that recognizes lactobacilli, on the development of atopic dermatitis (AD) and 

sensitization in infancy. 

DESIGN 

The participants of this study are a subcohort of the KOALA Birth Cohort Study and they are all children 

for whom both a faecal sample, at age 1 month, and a buccal swab sample were available. The KOALA 

Birth Cohort Study is a prospective cohort in the Netherlands,described in detail by Kummeling et al. 

(2005). 


Faecal samples of 681 one-month-old infants were collected and quantitatively screened for five 

Lactobacillus species: L. casei, L. paracasei, L. rhamnosus, L. acidophilus and L. reuteri. Eleven haplotypetagging 

polymorphisms in the DC-SIGN gene were genotyped in these children. Information on AD was 

available for all 506 children, whereas information on atopic sensitization was available for 492 children. 

MAIN OUTCOME 

Faecal microbiota composition, clinical diagnosis of AD and sensitization (specific IgE) at 2 years of age. 

RESULTS 

L. rhamnosus (31.5%), L. paracasei (31.3%) and L. acidophilus (14.4%) were frequently detected in 

the faecal samples of one-month-old infants, whereas L. casei (2.5%) and L. reuteri (1%) were rare. 

Colonization with L. paracasei decreased the risk of AD significantly (odds ratio 0.57, 95% CI 0.32- 0.99, 

P=0.04), whereas effects of L. acidophilus were of borderline statistical significance (0.46, 0.20-1.04, 

P=0.06). Two DC-SIGN polymorphisms, rs11465413 and rs8112555, were statistically significantly 

associated with atopic sensitization. 

CONCLUSION 

These results support the ‘‘old friends’’ hypothesis suggesting that certain health-beneficial microorganisms 

protect the host from developing allergies and that these protective effects are speciesdependent. 

Firm conclusions on the potential interaction between Lactobacillus colonization and genetic 

variations in DC-SIGN in association with the development of allergic disorders cannot be drawn. 

Penders J, Thijs C, Mommers M, Stobberingh EE, Dompeling E, Reijmerink NE, van den Brandt PA, Kerkhof M, Koppelman GH, Postma 

DS. Intestinal lactobacilli and the DC-SIGN gene for their recognition by dendritic cells play a role in the aetiology of allergic manifestations. 

Microbiology. 2010 Nov;156(Pt 11):3298-305. 

Kummeling I., Thijs C., Penders J., Snijders B. E., Stelma F., Reimerink J., Koopmans M., Dagnelie PC., Huber M. & other authors (2005). Etiology 

of atopy in infancy: the KOALA Birth Cohort Study. Pediatr Allergy Immunol 16, 679–684. 

COMMENTARY 

from Bruno Pot, Institut Pasteur de Lille, France 

«This study examined the effect of five common intestinal Lactobacillus species (of 17 species that can be 

present in the human GIT) in early infancy and looked into genetic variations in DC-SIGN, both in association 

with development of atopic dermatitis (AD) and allergic sensitization in early childhood, based on a clinical 

diagnosis of AD and sensitization (specifi IgE) at the age of 2. From the KOALA Birth Cohort Study, faecal 

samples from 681 one-month-old infants were quantitatively screened for Lactobacillus casei, L. paracasei, 

L. rhamnosus, L. acidophilus and L. reuteri. Contrary to what is stated in the discussion section, results show 

that colonization with L. paracasei significantly decreased the risk of AD (odds ratio 0.57, p= 0.04), whereas 

effects of L. acidophilus were of borderline statistical significance (0.46, p=0.06). 

The paper suggested that protective effects are species-dependent during childhood, but when considering 

probiotic applications, one is tempted to go even further and consider strain specificity rather than species 

specificity. Strain specificity could explain why, contrary to what is suggested in the paper, there are 

L. rhamnosus strains that do seem to have an effect on AD while L. acidophilus strain LAVRI-A1 e.g. did not 

show any effect in clinical trials. 

In the same population, two recognition receptor DC-SIGN SNPs, rs11465413 and rs8112555, were found to 

be significantly associated with atopic sensitization. 

Given the increased knowledge on mechanisms of interaction between the microbiota and the host, and the 

importance of these interactions at an early age when the immune system is maturing, it would be extremely 

interesting to examine possible associations between host genetics and microbiota composition in other 

populations in the future. » 

10



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In at risk children, LGG failed to prevent allergic manifestations 


Probiotics are perceived to exert beneficial effects in the prevention and treatment of allergic diseases. 

However the data from studies as to an impact on allergic sensitization and asthma is still conflicting. 

This trial examined the impact of probiotic Lactobacillus rhamnosus GG (LGG) on atopic dermatitis, 

asthma, and allergic sensitization in at risk children. 

DESIGN 

Randomized, double-blind, placebo controlled trial. 


131 children (6-24 months old) with at least two wheezing episodes and a first-degree family history of 

atopic disease were assigned to 6 months of LGG or placebo. Atopic dermatitis and asthma-related 

events (e.g. need of inhalation, symptom-free days) were documented throughout the intervention and 

6-month follow-up. The laboratory measures taken were: IgE, specific IgE, eosinophils, eosinophilic 

cationic protein, and TGF-b before, at the end of intervention, and after 6 months of follow-up. 

MAIN OUTCOME 

Primary outcome measures were the SCORAD and asthma-related clinical events, including an asthma 

symptom score defined by coughing, wheezing, and need of intervention. Secondary measures were 

allergic sensitization to common dietary and respiratory allergens. 

RESULTS 

There were no significant differences as to atopic dermatitis or asthma-related events. In a subgroup with 

antecedent allergic sensitizations, asthmatic complaints were even slightly worse. Fewer sensitizations 

towards aeroallergens were seen after 6 months of LGG (P=0.027) and after 6 months of follow-up 

(P=0.03). Supplementation was well-tolerated and no severe adverse events occurred. 

CONCLUSION 

In young children with recurrent wheezing and an atopic family history, oral L. rhamnosus GG had no 

clinical effect on atopic dermatitis or asthma-related events, and only mild effects on allergic sensitization. 

This effect persisted 6 months after the cessation of the supplementation. 

Rose MA, Stieglitz F, Köksal A, Schubert R, Schulze J, Zielen S. Efficacy of probiotic Lactobacillus GG on allergic sensitization and asthma in 

infants at risk. Clin Exp Allergy. 2010 Sep;40(9):1398-405. 

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3.3. IBS & FUNCTIONAL GI DISORDERS 

Multispecies probiotic intervention alleviates bacterial alterations 

in IBS patients 


The current hypothesis is that probiotics can alleviate the symptoms of irritable bowel syndrome (IBS), 

possibly by stabilizing the intestinal microbiota. The aim of this work was to determine whether IBSassociated 

bacterial alterations were reduced during multispecies probiotic intervention. 

DESIGN 

Randomized, double-blind, placebo-controlled intervention. 


42 IBS patients (mean age 46 yrs; 29 females and 13 males) received daily (during 6 monts) either a 

probiotic capsule consisting of Lactobacillus rhamnosus GG (ATCC 53103), L. rhamnosus Lc705 (DSM 

7061), Propionibacterium freudenreichii ssp. shermanii JS (DSM 7067) and Bifidobacterium breve Bb99 

(DSM 13692) or a placebo capsule. Faecal samples were analysed using quantitative real-time polymerase 

chain reaction (qPCR). Eight bacterial targets within the gastrointestinal microbiota with a putative IBS 

association were measured (Bacteroides intestinalis-like, Clostridium cocleatum 88%, Clostridium 

thermosuccinogenes, Collinsella aerofaciens-like, Coprococcus eutactus-like, Ruminococcus torques 

91%, R. torques 93% and R. torques 94%) 

MAIN OUTCOME 

Analysis of faecal microbiota. 

RESULTS 

A phylotype with 94% similarity to R. torques remained abundant in the placebo group, but was decreased 

in the probiotic group during the intervention (P=0.02 at 6 months). In addition, the clostridial phylotype, 

C. thermosuccinogenes 85%, remained elevated during the intervention (P=0.00 and P=0.02 at 3 and 6 

months, respectively). The bacterial alterations detected were in line with previously discovered alleviation 

of symptoms. 

CONCLUSION 

The intervention has previously been shown to successfully alleviate gastrointestinal symptoms of IBS. 

The probiotic supplement was now shown to exert specific alterations in the IBS-associated microbiota 

towards the bacterial 16S rDNA phylotype quantities described previously for subjects free of IBS. These 

changes may have value as non-invasive biomarkers in probiotic intervention studies. 

Lyra A, Krogius-Kurikka L, Nikkilä J, Malinen E, Kajander K, Kurikka K, Korpela R, Palva A. Effect of a multispecies probiotic supplement on 

quantity of irritable bowel syndrome-related intestinal microbial phylotypes. BMC Gastroenterol. 2010 Sep 19;10:110. 

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L. rhamnosus GG relieves symptoms of abdominal pain in IBS 

children 


Recurrent abdominal pain is primarily a functional disorder that affects 10% to 15% of school-aged 

children and is one of the most common reasons for referral to a pediatric gastroenterologist. Data on 

the possible use of probiotics in adults with irritable bowel syndrome (IBS) have hypothetisized their 

possible efficacy. The aim of the present study is to determine whether Lactobacillus rhamnosus GG 

(LGG) relieves symptoms in children with recurrent abdominal pain. 

DESIGN 

Multicentric, randomized, double-blind, placebo controlled trial. 


A total of 141 children (5-14 years) with IBS or functional pain were enrolled in 9 primary care sites. 

Children received LGG or a placebo for 8 weeks and entered follow-up for 8 weeks. At the start and at 

the end of the trial, children underwent a double-sugar intestinal permeability test. 

MAIN OUTCOME 

The primary outcome was overall pain at the end of the intervention period. 

RESULTS 

Compared with baseline, LGG, but not placebo, caused a significant reduction in both the frequency 

(P




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3.4. IBD AND OTHER INFLAMMATORY DISEASES 

A mechanism explaining the preventive effect of probiotics against colitis 

OBJECTIVES 

Intestinal health requires the coexistence of the host’s own cells with the gut microbiota, and dysregulated 

host-microbial interactions can result in intestinal inflammation. This study used a murine model of colitis to 

gain insight into how a defined probiotic-containing food reduces intestinal inflammation, and focused on the 

microbiota and its response to host consumption of specific probiotics. 


T-bet −/− Rag2 −/− mice consumed a fermented milk product (BFMP) containing Bifidobacterium lactis DN-173 

010, Streptococcus thermophilus, Lactobacillus bulgaricus, and Lactococcus lactis or a nonfermented milk 

product (MP) starting at 4 wk of age for 4 wk. 

RESULTS 

The BFMP reduced colitis scores in the majority of mice both at early stages and at late stages of disease when 

intestinal inflammation was more severe. Colitis scores in the live BFMP were lower than those of the irradiated 

product (P=0.03). 

BFMP consumption decreased faecal levels of Enterobacteriaceae sevenfold (P=0.0132) compared to MP. 

The cecal pH of T-bet −/− Rag2 −/− mice was markedly lower after BFMP consumption (P




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Identification of a set of microbial species specific to Crohn’s 

disease condition 

OBJECTIVES 

Crohn’s disease (CD) is a multifactorial disease of the human digestive tract whose etiology is still unclear. 

Several studies suggest that gastrointestinal microbes play a part in this pathogenesis and hypothesized 

that intestinal dysbiosis may lead to this condition. The aim of this study was to provide new insights into 

CD-associated dysbiosis by combining molecular approaches. 

METHOD & MEASURES 

Faecal samples were collected from 16 healthy individuals and 16 CD patients. The DNA extracted 

from these samples was subjected to two different methods of microbiome analysis. Specific bacterial 

groups were quantified by RT-PCR methods using primers designed using a high-throughput in-house 

bioinformatics pipeline. The same DNA extracts were also used to produce fluorescently-labelled cRNA 

amplicons to interrogate a custom-designed phylogenetic microarray for intestinal bacteria. 

RESULTS 

Even though the intersubject variability was high, differences in the faecal microbiomes of healthy and 

CD patients were detected. Faecalibacterium prausnitzii and Escherichia coli were more represented in 

healthy and ileal CD patients, respectively. Additionally, probes specific for Ruminococcus bromii, 

Oscillibacter valericigenes, Bifidobacterium bifidum, and Eubacterium rectale produced stronger 

hybridization signals with the DNA samples from healthy subjects. Conversely, species overrepresented 

in CD patients were E. coli, Enterococcus faecium, and species from the Proteobacteria not normally 

found in the healthy human GI tract. Furthermore, ‘‘healthy specific’’ molecular species or operational 

taxonomic units that are not closely-related to any known species (Faecalibacterium, Subdoligranulum, 

and Oscillospora species) were detected, indicating that the phylogenetic dysbiosis is broader than at 

strain or species level. 

CONCLUSION 

These two techniques of microbiome analysis provided a statistically-robust new picture of the dysbiosis 

in faecal microbiota from ileal CD patients. Specifically, a set of six species discriminant for CD was 

determined, which provides a preliminary diagnostic tool. 

Mondot S, Kang S, Furet JP, Aguirre de Carcer D, McSweeney C, Morrison M, Marteau P, Doré J, Leclerc M. Highlighting new phylogenetic 

specificities of Crohn’s disease microbiota. Inflamm Bowel Dis. 2010 Aug 18. 

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L. reuteri consumption improves colic symptoms in breastfed 

newborns 


Infantile colic, defined as paroxysmal, excessive, and inconsolable crying without an identifiable cause 

in an otherwise healthy newborn infant, is common in the first 3 months of life. The role of an aberrant 

intestinal microflora has recently been reproposed to affect gut motor function and gas production that 

lead to colicky behaviour. This work aimed to test the efficacy of Lactobacillus reuteri on infantile colic 

and to evaluate its relationship with gut microbiota. 

DESIGN 



Fifty exclusively breastfed colicky infants (aged 2-16 weeks, 29 boys and 21 girls), diagnosed according 

to modified Wessel’s criteria, were randomly assigned to receive either L. reuteri DSM 17 938 (108 CFU) 

or a placebo daily for 21 days. Parental questionnaires monitored daily crying time and adverse effects. 

Stool samples were collected for microbiologic analysis. 

MAIN OUTCOME 

Primary outcome was defined as a reduction of average crying time to




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3.5. NUTRITION: OBESITY, CHOLESTEROL, ETC. 

Probiotics against central adiposity in pregnant women 

OBJECTIVES/BACKGOUND 

The objective of this trial was to establish whether probiotic supplemented dietary counselling influences 

maternal anthropometric measurements during and after pregnancy. 

DESIGN 



At the first trimester of pregnancy, 256 women were randomly assigned to receive nutrition counselling to 

modify dietary intake according to current recommendations or as controls. Dietary intervention groups 

were further randomized to receive Lactobacillus rhamnosus GG (ATCC 53103) and Bifidobacterium 

lactis (diet/probiotics) or placebo (diet/placebo) capsules, whilst the controls received placebo (control/ 

placebo). The intervention lasted until the end of exclusive breastfeeding for up to six months. 

MAIN OUTCOME 

The primary outcome measures were mothers’ anthropometric measurements, most importantly BMI 

and adiposity defined as a waist circumference of 80 cm or more, and the proportion of body fat over 

the 12-month postpartum period. Secondary outcomes were dietary intakes of foods and nutrients and 

a healthy eating index during the postpartum period. 

RESULTS 

The risk of central adiposity was lowered in women in the diet/probiotics group compared with the 

control/placebo group (OR 0.30, 95%CI 0.11-0.85, P=0.023 adjusted for baseline BMI), whilst the 

diet/placebo group did not differ from the controls (OR 1.00, 95% CI 0.38-2.68, P=0.994) at 6 months 

postpartum. The number needed to treat with diet/ probiotics to prevent one woman from developing a 

waist circumference ≥80 cm was 4. Healthy eating pattern at 12 months postpartum (P=0.001) and BMI 

prior to pregnancy (P




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KEY DATES 

Control of mothers’ diet during pregnancy benefits metabolic 

health of infants 


This trial aimed to evaluate the impact of maternal diet and intensive dietary counselling during pregnancy 

and breastfeeding on the infant’s metabolic status. 

DESIGN 



At the first trimester of pregnancy, 256 women were randomized into a control/placebo group and two 

dietary counselling (diet/probiotics and diet/placebo). The counselling, with double-blind randomization 

with probiotics (Lactobacillus rhamnosus GG and Bifidobacterium lactis) or placebo, targeted excessive 

saturated fat and low fibre consumption. Maternal diet was evaluated repeatedly during pregnancy and 

postpartum by means of 3-day food diaries. Metabolic markers, serum 32-33 split and intact proinsulin, 

leptin/adiponectin ratio, skinfold thickness and waist circumference were measured of 194 healthy infants 

at the age of 6 months, and the high levels were taken to mirror adverse metabolic status. 

MAIN OUTCOME 

The serum 32-33 split and intact proinsulin concentrations were primary outcome variables, leptin and 

adiponectin concentrations, waist circumference and supra-iliac skinfold were secondary outcome 

variables. Any 32-33 split and intact proinsulin values above the 85 th percentile of the concentrations (7.9 

and 6.64 pmol/l, respectively) were considered high values. 

RESULTS 

The proportion of infants with a high 32-33 split proinsulin was significantly lower in dietary counselling 

groups with probiotics (n=6/62, 9.7%) or placebo (n=7/69, 10.1%) compared with the control/placebo 

group (n=17/63, 27.0%). The high split proinsulin was associated with larger skinfold thickness, waist 

circumference and higher leptin/adiponectin ratios in the infants (P



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Mothers’ weight or weight gain during pregnancy impacts the 

intestinal flora composition of the infants 


It has been reported that deviations in gut microbiota composition may predispose to obesity, and 

specific groups of commensal gut bacteria may harvest energy from food more efficiently than others. 

Alterations in microbiota compositions of mothers may be transferred to infants and lead to an increased 

risk of overweight. The aim of this study is to analyze the faecal microbiota composition of infants of 

overweight and normal-weight mothers and assess the relations of weight and excessive weight gain of 

mothers during pregnancy on the microbiota of infants. 

DESIGN 

Obervational study. 


Mothers (n=16) whose pre-pregnancy body mass index (BMI) was ≥25 were selected with their infants 

from a prospective follow-up study of 256 women. Women with a BMI



ISSUE N°4 REFERENCES 

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REFERENCES 

KEY DATES 

CHARACTERISATION OF PROBIOTICS AND PROBIOTIC FOODS 

STRAIN IDENTIFICATION AND CHARACTERISATION 

Büchl NR, Hutzler M, Mietke-Hofmann H, Wenning M, Scherer S. 

Differentiation of probiotic and environmental Saccharomyces cerevisiae 

strains in animal feed. J Appl Microbiol. 2010 Sep;109(3):783-91. doi: 

10.1111/j.1365-2672.2010.04705.x. 

Cai Y, Yang J, Pang H, Kitahara M. Lactococcus fujiensis sp nov., a 

lactic acid bacterium isolated from vegetable. Int J Syst Evol Microbiol. 

2010 Jul 30. 

Chen YS, Miyashita M, Suzuki K, Sato H, Hsu JS, Yanagida F. 

Lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented 

cummingcordia). Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1914-7. 

De Bruyne K, Camu N, De Vuyst L, Vandamme P. Weissella fabaria sp. 

nov., from a Ghanaian cocoa fermentation. Int J Syst Evol Microbiol. 

2010 Sep;60(Pt 9):1999-2005. 

Di Cagno R, Minervini G, Sgarbi E, Lazzi C, Bernini V, Neviani E, Gobbetti 

M. Comparison of phenotypic (Biolog System) and genotypic (random 

amplified polymorphic DNA-polymerase chain reaction, RAPD-PCR, 

and amplified fragment length polymorphism, AFLP) methods for 

typing Lactobacillus plantarum isolates from raw vegetables and fruits. 

Int J Food Microbiol. 2010 Oct 15;143(3):246-53. 

Ehrmann MA, Preissler P, Danne M, Vogel RF. Lactobacillus 

paucivorans sp. nov., isolated from a brewery environment. Int J Syst 

Evol Microbiol. 2010 Oct;60(Pt 10):2353-7. 

Fujimoto J, Tanigawa K, Kudo Y, Makino H, Watanabe K. 

Identification and quantification of viable Bifidobacterium breve 

strain Yakult in human faeces by using strain-specific primers 

and propidium monoazide. J Appl Microbiol. 2010 Sep 27. doi: 

10.1111/j.1365-2672.2010.04873.x. 

Gilad O, Jacobsen S, Stuer-Lauridsen B, Pedersen MB, Garrigues 

C, Svensson B. Combined transcriptome and proteome analysis 

of Bifidobacterium animalis subsp. lactis BB-12 grown on xylooligosaccharides 

and a model of their utilization. Appl Environ 

Microbiol. 2010 Nov;76(21):7285-91. 

Huang CH, Lee FL. The dnaK gene as a molecular marker for the 

classification and discrimination of the Lactobacillus casei group. 

Antonie Van Leeuwenhoek. 2010 Aug 11. 

Killer J, Kopecný J, Mrázek J, Havlík J, Koppová I, Benada O, Rada 

V, Kofrocová O. Bombiscardovia coagulans gen. nov., sp. nov., a new 

member of the family Bifidobacteriaceae isolated from the digestive 

tract of bumblebees. Syst Appl Microbiol. 2010 Oct 13. 

Lai D, Yang X, Wu G, Liu Y, Nardini C. Inference of Gene Networks - 

application to Bifidobacterium. Bioinformatics. 2010 Nov 12. 

Markiewicz LH, Biedrzycka E, Wasilewska E, Bielecka M. Rapid 

molecular identification and characteristics of Lactobacillus strains. 

Folia Microbiol (Praha). 2010 Sep;55(5):481-8. 

Marques AP, Zé-Zé L, San-Romão MV, Tenreiro R. A novel molecular 

method for identification of Oenococcus oeni and its specific detection 

in wine. Int J Food Microbiol. 2010 Aug 15;142(1-2):251-5. 

Samelis J, Bleicher A, Delbès-Paus C, Kakouri A, Neuhaus K, Montel 

MC. FTIR-based polyphasic identification of lactic acid bacteria 

isolated from traditional Greek Graviera cheese. Food Microbiol. 2011 

Feb;28(1):76-83. 

Sarmiento-Rubiano LA, Berger B, Moine D, Zúñiga M, Pérez-Martínez 

G, Yebra MJ. Characterization of a novel Lactobacillus species closely 

related to Lactobacillus johnsonii using a combination of molecular 

and comparative genomics methods. BMC Genomics. 2010 Sep 

17;11:504. 

Sato H, Teramoto K, Ishii Y, Watanabe K, Benno Y. Ribosomal protein 

profiling by matrix-assisted laser desorption/ionization time-of-flight 

mass spectrometry for phylogenety-based subspecies resolution of 

Bifidobacterium longum. Syst Appl Microbiol. 2010 Sep 7. 

Wang CY, Lin PR, Ng CC, Shyu YT. Probiotic properties of Lactobacillus 

strains isolated from the feces of breast-fed infants and Taiwanese 

pickled cabbage. Anaerobe. 2010 Oct 15. 

STRAIN METABOLISM AND PHYSIOLOGY 

Arai T, Kino K. New L-amino Acid ligases catalyzing oligopeptide 

synthesis from various microorganisms. Biosci Biotechnol Biochem. 

2010 Aug 23;74(8):1572-7. 

Candela M, Fiori J, Dipalo S, Brigidi P. Development of a highperformance 

affinity chromatography-based method to study the 

biological interaction between whole micro-organisms and target 

proteins. Lett Appl Microbiol. 2010 Oct 6. doi: 10.1111/j.1472- 

765X.2010.02953.x. 

Carvalheiro F, Moniz P, Duarte LC, Esteves MP, Gírio FM. Mannitol 

production by lactic acid bacteria grown in supplemented carob syrup. 

J Ind Microbiol Biotechnol. 2010 Sep 5. 

Chu W, Lu F, Zhu W, Kang C. Isolation and characterization of new 

potential probiotic bacteria based on quorum-sensing system. J Appl 

Microbiol. 2010 Sep 27. doi: 10.1111/j.1365-2672.2010.04872.x. 

Endo A, Futagawa-Endo Y, Sakamoto M, Kitahara M, Dicks LM. 

Lactobacillus florum sp. nov., a fructophilic species isolated from 

flowers. Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2478-82. 

Fukiya S, Sugiyama T, Kano Y, Yokota A. Characterization of an insertion 

sequence-like element, ISBlo15, identified in a size-increased cryptic 

plasmid pBK283 in Bifidobacterium longum BK28. J Biosci Bioeng. 

2010 Aug;110(2):141-6. 

Garneau JE, Dupuis MÈ, Villion M, Romero DA, Barrangou R, Boyaval 

P, Fremaux C, Horvath P, Magadán AH, Moineau S. The CRISPR/Cas 

bacterial immune system cleaves bacteriophage and plasmid DNA. 

Nature. 2010 Nov 4;468(7320):67-71.. 

Ge XY, Yuan J, Qin H, Zhang WG. Improvement of L: -lactic acid 

production by osmotic-tolerant mutant of Lactobacillus casei at high 

temperature. Appl Microbiol Biotechnol. 2010 Sep 21. 

Goffin P, van de Bunt B, Giovane M, Leveau JH, Höppener-Ogawa S, 

Teusink B, Hugenholtz J. Understanding the physiology of Lactobacillus 

plantarum at zero growth. Mol Syst Biol. 2010 Sep 21;6:413. 

Hancock V, Vejborg RM, Klemm P. Functional genomics of probiotic 

Escherichia coli Nissle 1917 and 83972, and UPEC strain CFT073: 

comparison of transcriptomes, growth and biofilm formation. Mol 

Genet Genomics. 2010 Oct 1. 

Harris PT, Raghunathan K, Spurbeck RR, Arvidson CG, Arvidson DN. 

Expression, purification, crystallization and preliminary X-ray studies 

of Lactobacillus jensenii enolase. Acta Crystallogr Sect F Struct Biol 

Cryst Commun. 2010 Aug 1;66(Pt 8):938-40. 

Horsham M, Saxby H, Blake J, Isaacs NW, Mitchell TJ, Riboldi- 

Tunnicliffe A. Expression, purification, crystallization and preliminary 

X-ray crystallographic data from TktA, a transketolase from the lactic 

acid bacterium Lactobacillus salivarius. Acta Crystallogr Sect F Struct 

Biol Cryst Commun. 2010 Aug 1;66(Pt 8):899-901. 

Ismail B, Nampoothiri KM. Production, purification and structural 

characterization of an exopolysaccharide produced by a probiotic 

Lactobacillus plantarum MTCC 9510. Arch Microbiol. 2010 Oct 19. 

Jeon JM, Lee HI, Han SH, Chang CS, So JS. Partial purification and 

characterization of glutaminase from Lactobacillus reuteri KCTC3594. 

Appl Biochem Biotechnol. 2010 Sep;162(1):146-54. 

Jia J, Mu W, Zhang T, Jiang B. Bioconversion of phenylpyruvate to 

phenyllactate: gene cloning, expression, and enzymatic characterization 

of D- and L1-lactate dehydrogenases from Lactobacillus plantarum 

SK002. Appl Biochem Biotechnol. 2010 Sep;162(1):242-51. 

Jiménez E, Martín R, Maldonado A, Martín V, Gómez de Segura 

A, Fernández L, Rodríguez JM. Complete genome sequence of 

Lactobacillus salivarius CECT 5713, a probiotic strain isolated from 

human milk and infant feces. J Bacteriol. 2010 Oct;192(19):5266-7. 

Jin J, Liu S, Zhao L, Ge K, Mao X, Ren F. Changes in ffh, uvrA, groES 

and dnaK mRNA Abundance as a Function of Acid-Adaptation and 

Growth Phase in Bifidobacterium longum BBMN68 Isolated from 

Healthy Centenarians. Curr Microbiol. 2010 Sep 11. 

Kimoto-Nira H, Suzuki C, Sasaki K, Kobayashi M, Mizumachi K. Survival 

of a Lactococcus lactis strain varies with its carbohydrate preference 

under in vitro conditions simulated gastrointestinal tract. Int J Food 

Microbiol. 2010 Oct 15;143(3):226-9. 

Kiyohara M, Tanigawa K, Chaiwangsri T, Katayama T, Ashida H, 

Yamamoto K. An exo-{alpha}-sialidase from bifidobacteria involved in 

the degradation of sialyloligosaccharides in human milk and intestinal 

glycoconjugates. Glycobiology. 2010 Oct 29. 

Koskenniemi K, Laakso K, Koponen J, Kankainen M, Greco D, 

Auvinen P, Savijoki K, Nyman TA, Surakka A, Salusjärvi T, de Vos WM, 

Tynkkynen S, Kalkkinen N, Varmanen P. Proteomic and transcriptomic 

characterization of bile stress response in probiotic Lactobacillus 

rhamnosus GG. Mol Cell Proteomics. 2010 Nov 15. 

Kullisaar T, Songisepp E, Aunapuu M, Kilk K, Arend A, Mikelsaar 

M, Rehema A, Zilmer M. Complete glutathione system in probiotic 

Lactobacillus fermentum ME-3. Prikl Biokhim Mikrobiol. 2010 Sep- 

Oct;46(5):527-31. 

Kuratsu M, Hamano Y, Dairi T. Analysis of the Lactobacillus metabolic 

pathway. Appl Environ Microbiol. 2010 Nov;76(21):7299-301. 

Lagaert S, Pollet A, Delcour JA, Lavigne R, Courtin CM, Volckaert G. 

Substrate specificity of three recombinant �-l-arabinofuranosidases 

from Bifidobacterium adolescentis and their divergent action on 

arabinoxylan and arabinoxylan oligosaccharides. Biochem Biophys 

Res Commun. 2010 Oct 28. 

Łaniewska-Trokenheim Ł, Olszewska M, Miks-Krajnik M, Zadernowska 

A. Patterns of survival and volatile metabolites of selected Lactobacillus 

strains during long-term incubation in milk. J Microbiol. 2010 

Aug;48(4):445-51. 

20




Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Li H, Qiu T, Huang G, Cao Y. Production of gamma-aminobutyric acid 

by Lactobacillus brevis NCL912 using fed-batch fermentation. Microb 

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of Milk Utilization Genes in Bifidobacterium longum subsp. infantis 

as Revealed by Comparative Genomic Hybridization. Appl Environ 

Microbiol. 2010 Nov;76(22):7373-81. 

López de Felipe F, Curiel JA, Muñoz R. Improvement of the 

fermentation performance of Lactobacillus plantarum by the flavanol 

catechin is uncoupled from its degradation. J Appl Microbiol. 2010 

Aug;109(2):687-97. 

Luo LH, Seo JW, Baek JO, Oh BR, Heo SY, Hong WK, Kim DH, Kim 

CH. Identification and characterization of the propanediol utilization 

protein PduP of Lactobacillus reuteri for 3-hydroxypropionic acid 

production from glycerol. Appl Microbiol Biotechnol. 2010 Oct 2. 

Mackenzie DA, Jeffers F, Parker ML, Vibert-Vallet A, Bongaerts RJ, 

Roos S, Walter J, Juge N. Strain-specific diversity of mucus-binding 

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reuteri. Microbiology. 2010 Nov;156(Pt 11):3368-78. 

Maischberger T, Leitner E, Nitisinprasert S, Juajun O, Yamabhai 

M, Nguyen TH, Haltrich D. Beta-galactosidase from Lactobacillus 

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29;285(44):33906-13. 

Nakai H, Petersen BO, Westphal Y, Dilokpimol A, Abou Hachem M, 

Duus JØ, Schols HA, Svensson B. Rational engineering of Lactobacillus 

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Nakanishi Y, Fukuda S, Chikayama E, Kimura Y, Ohno H, Kikuchi J. 

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Towards enhanced galactose utilization by Lactococcus lactis. Appl 

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Palomino MM, Allievi MC, Prado-Acosta M, Sanchez-Rivas C, Ruzal 

SM. New method for electroporation of Lactobacillus species grown in 

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Parente E, Ciocia F, Ricciardi A, Zotta T, Felis GE, Torriani S. Diversity 

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Rekha CR, Vijayalakshmi G. Bioconversion of isoflavone glycosides to 

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Rodríguez N, Torrado A, Cortés S, Domínguez JM. Use of waste 

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Aug 15;90(10):1726-34. 

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Shimoda K, Hamada H. Synthesis of beta-maltooligosaccharides of 

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Molecules. 2010 Jul 29;15(8):5153-61. 

Sieuwerts S, Molenaar D, van Hijum SA, Beerthuyzen M, Stevens MJ, 

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Stephenson DP, Moore RJ, Allison GE. Transformation of, and 

heterologous protein expression in, Lactobacillus agilis and 

Lactobacillus vaginalis isolated from the chicken gastrointestinal tract. 

Appl Environ Microbiol. 2010 Nov 12. 

Streit F, Athès V, Bchir A, Corrieu G, Béal C. Microfiltration conditions 

modify Lactobacillus bulgaricus cryotolerance in response to 

physiological changes. Bioprocess Biosyst Eng. 2010 Aug 28. 

Sudiarta IP, Fukushima T, Sekiguchi J. bacillus subtilis CwlP of the 

SP-beta prophage has two novel peptidoglycan hydrolase domains, 

muramidase and cross-linkage digesting D,D-endopeptidase. J Biol 

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Suzuki R, Kim BJ, Shibata T, Iwamoto Y, Katayama T, Ashida H, 

Wakagi T, Shoun H, Fushinobu S, Yamamoto K. Overexpression, 

crystallization and preliminary X-ray analysis of xylulose-5-phosphate/ 

fructose-6-phosphate phosphoketolase from Bifidobacterium breve. 

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt 

8):941-3. 

Takahashi K, Tagami U, Shimba N, Kashiwagi T, Ishikawa K, Suzuki 

E. Crystal structure of Bifidobacterium Longum phosphoketolase; key 

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Sep 24;584(18):3855-61. 

Tang AL, Wilcox G, Walker KZ, Shah NP, Ashton JF, Stojanovska L. 

Phytase Activity from Lactobacillus spp. in calcium-fortified soymilk. J 

Food Sci. 2010 Aug 1;75(6):M373-6. 

Turroni F, Bottacini F, Foroni E, Mulder I, Kim JH, Zomer A, Sánchez B, 

Bidossi A, Ferrarini A, Giubellini V, Delledonne M, Henrissat B, Coutinho 

P, Oggioni M, Fitzgerald GF, Mills D, Margolles A, Kelly D, van Sinderen 

D, Ventura M. Genome analysis of Bifidobacterium bifidum PRL2010 

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Vebø HC, Solheim M, Snipen L, Nes IF, Brede DA. Comparative 

genomic analysis of pathogenic and probiotic Enterococcus faecalis 

isolates, and their transcriptional responses to growth in human urine. 

PLoS One. 2010 Aug 31;5(8):e12489. 

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Aug;59(3):386-98. 

Waldherr FW, Doll VM, Meissner D, Vogel RF. Identification and 

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hilgardii TMW 1.828 involved in granule formation of water kefir. Food 

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Wang G, Yin S, An H, Chen S, Hao Y. Coexpression of bile salt 

hydrolase gene and catalase gene remarkably improves oxidative 

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Wang Y, Chen C, Ai L, Zhou F, Zhou Z, Wang L, Zhang H, Chen W, Guo 

B. Complete genome sequence of probiotic Lactobacillus plantarum 

ST-III. J Bacteriol. 2010 Oct 29. 

Wasko A, Kordowska-Wiater M, Podlesny M, Polak-Berecka M, 

Targonski Z, Kubik-Komar A. The Plackett-Burman design in optimization 

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Wasko A, Polak-Berecka M, Targonski Z. A new protein of alphaamylase 

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Sep;20(9):1307-13. 

Weidmann S, Rieu A, Rega M, Coucheney F, Guzzo J. Distinct amino 

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Epithelial Signaling Mediated via Formyl Peptide Receptors. Am J 

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Wu R, Sun Z, Wu J, Meng H, Zhang H. Effect of bile salts stress on protein 

synthesis of Lactobacillus casei Zhang revealed by 2-dimensional gel 

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Yang EJ, Chang HC. Construction and evaluation of shuttle vector, 

pGYC4�, based on pYC2 from Lactobacillus sakei. Biotechnol Lett. 

2010 Nov 12. 

Zhang C, Gänzle MG. Metabolic pathway of �-ketoglutarate in 

Lactobacillus sanfranciscensis and Lactobacillus reuteri during 

sourdough fermentation. J Appl Microbiol. 2010 Oct;109(4):1301-10. 

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Zhang H, Hao Y, Zhang D, Luo Y. Characterization of the cryptic plasmid 

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21




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REFERENCES 

KEY DATES 

Zhang W, Yu D, Sun Z, Wu R, Chen X, Chen W, Meng H, Hu S, Zhang 

H. Complete genome sequence of Lactobacillus casei Zhang, a new 

probiotic strain isolated from traditional homemade koumiss in Inner 

Mongolia, China. J Bacteriol. 2010 Oct;192(19):5268-9. 

Zhurina D, Zomer A, Gleinser M, Brancaccio VF, Auchter M, Waidmann 

MS, Westermann C, van Sinderen D, Riedel CU. Complete genome 

sequence of Bifidobacterium bifidum S17. J Bacteriol. 2010 Oct 29. 

TECHNOLOGY OF PROBIOTIC AND PROBIOTIC FOODS 

Albenzio M, Santillo A, Caroprese M, Marino R, Trani A, Faccia M. 

Biochemical patterns in ovine cheese: influence of probiotic strains. J 

Dairy Sci. 2010 Aug;93(8):3487-96. 

Bolla PA, de Los Angeles Serradell M, de Urraza PJ, De Antoni GL. 

Effect of freeze-drying on viability and in vitro probiotic properties of 

a mixture of lactic acid bacteria and yeasts isolated from kefir. J Dairy 

Res. 2010 Sep 8:1-8. 

Brachkova MI, Duarte MA, Pinto JF. Preservation of viability and 

antibacterial activity of Lactobacillus spp. in calcium alginate beads. 

Eur J Pharm Sci. 2010 Aug 26. 

Burns P, Molinari F, Beccaria A, Páez R, Meinardi C, Reinheimer J, 

Vinderola G. Suitability of buttermilk for fermentation with Lactobacillus 

helveticus and production of a functional peptide-enriched powder 

by spray-drying. J Appl Microbiol. 2010 Oct;109(4):1370-8. doi: 

10.1111/j.1365-2672.2010.04761.x. Epub 2010 Aug 19. 

Champagne CP, Tompkins TA, Buckley ND, Green-Johnson JM. 

Effect of fermentation by pure and mixed cultures of Streptococcus 

thermophilus and Lactobacillus helveticus on isoflavone and 

B-vitamin content of a fermented soy beverage. Food Microbiol. 2010 

Oct;27(7):968-72. 

Chávarri M, Marañón I, Ares R, Ibáñez FC, Marzo F, Villarán Mdel C. 

Microencapsulation of a probiotic and prebiotic in alginate-chitosan 

capsules improves survival in simulated gastro-intestinal conditions. 

Int J Food Microbiol. 2010 Aug 15;142(1-2):185-9. 

Di Criscio T, Fratianni A, Mignogna R, Cinquanta L, Coppola R, 

Sorrentino E, Panfili G. Production of functional probiotic, prebiotic, 

and synbiotic ice creams. J Dairy Sci. 2010 Oct;93(10):4555-64. 

Grzeskowiak L, Isolauri E, Salminen S, Gueimonde M. Manufacturing 

process influences properties of probiotic bacteria. Br J Nutr. 2010 

Nov 9:1-8. 

Islam MA, Yun CH, Choi YJ, Cho CS. Microencapsulation of live 

probiotic bacteria. J Microbiol Biotechnol. 2010 Oct;20(10):1367-77. 

Meng XC, Pang R, Wang C, Wang LQ. Rapid and direct quantitative 

detection of viable bifidobacteria in probiotic yogurt by combination 

of ethidium monoazide and real-time PCR using a molecular beacon 

approach. J Dairy Res. 2010 Nov;77(4):498-504. 

Vanhee LM, Goemé F, Nelis HJ, Coenye T. Quality control of 

fifteen probiotic products containing Saccharomyces boulardii. 

J Appl Microbiol. 2010 Nov;109(5):1745-52. doi: 10.1111/j.1365- 

2672.2010.04805.x. 

PHYSIOLOGICAL INTERACTION WITH THE HOST 

SURVIVAL & PHYSICAL INTERACTIONS OF PROBIOTICS WITH MUCOSA 

Alp G, Aslim B, Suludere Z, Akca G. The role of hemagglutination and 

effect of exopolysaccharide production on bifidobacteria adhesion to 

Caco-2 cells in vitro. Microbiol Immunol. 2010 Nov;54(11):658-65. doi: 

10.1111/j.1348-0421.2010.00227.x.. 

Burns P, Sánchez B, Vinderola G, Ruas-Madiedo P, Ruiz L, Margolles 

A, Reinheimer J, de los Reyes-Gavilán CG. Inside the adaptation 

process of Lactobacillus delbrueckii subsp. lactis to bile. Int J Food 

Microbiol. 2010 Aug 15;142(1-2):132-41. 

Del Piano M, Carmagnola S, Andorno S, Pagliarulo M, Tari 

R, Mogna L, Strozzi GP, Sforza F, Capurso L. Evaluation of 

the intestinal colonization by microencapsulated probiotic 

bacteria in comparison with the same uncoated strains. J Clin 

Gastroenterol. 2010 Sep;44 Suppl 1:S42-6. 

Du L, Liu F, Ju X, Huo G. Adhesion capability of first two domains 

at N terminus of NP_785232 protein and their interaction with a UVabsorbing 

component from human mucus. Lett Appl Microbiol. 2010 

Oct;51(4):400-5. doi: 10.1111/j.1472-765X.2010.02911.x. 

Grimoud J, Durand H, Courtin C, Monsan P, Ouarné F, Theodorou 

V, Roques C. In vitro screening of probiotic lactic acid bacteria 

and prebiotic glucooligosaccharides to select effective synbiotics. 

Anaerobe. 2010 Oct;16(5):493-500. 

Hsueh HY, Yueh PY, Yu B, Zhao X, Liu JR. Expression of Lactobacillus 

reuteri Pg4 Collagen-Binding Protein Gene in Lactobacillus casei 

ATCC 393 Increases Its Adhesion Ability to Caco-2 Cells. J Agric Food 

Chem. 2010 Nov 11. 

Iguchi A, Umekawa N, Maegawa T, Tsuruta H, Odamaki T, Xiao JZ, 

Osawa R. Polymorphism and distribution of putative cell-surface 

adhesin-encoding ORFs among human faecal isolates of Bifidobacterium 

longum subsp. longum. Antonie Van Leeuwenhoek. 2010 Sep 24. 

Martín R, Olivares M, Pérez M, Xaus J, Torre C, Fernández L, Rodríguez 

JM. Identification and evaluation of the probiotic potential of lactobacilli 

isolated from canine milk. Vet J. 2010 Aug;185(2):193-8. 

Martins AK, Martins FS, Gomes DA, Elian SD, Vieira AT, Teixeira MM, 

Cara DC, Nardi RM, Nicoli JR. Evaluation of in vitro antagonism and 

of in vivo immune modulation and protection against pathogenic 

experimental challenge of two probiotic strains of Bifidobacterium 

animalis var. lactis. Arch Microbiol. 2010 Sep 17. 

Peleg M, Normand MD, Horowitz J, Corradini MG. Expanded fermi 

solution for estimating the survival of ingested pathogenic and probiotic 

microbial cells and spores. Appl Environ Microbiol. 2010 Nov 5. 

Samonis G, Falagas ME, Lionakis S, Ntaoukakis M, Kofteridis DP, 

Ntalas I, Maraki S. Saccharomyces boulardii and Candida albicans 

experimental colonization of the murine gut. Med Mycol. 2010 Nov 

15. 

Shen D, Zhu Y, Hao Y, Lu J. Polymerase chain reaction detection of 

Lactobacillus acidophilus in human oral cavity and faecal samples after 

2-week consumption of yoghurt. Acta Odontol Scand. 2010 Sep 23. 

Stephenson DP, Moore RJ, Allison GE. Lactobacillus strain ecology and 

persistence within broiler chickens fed different diets: identification of 

persistent strains. Appl Environ Microbiol. 2010 Oct;76(19):6494-503. 

Takemura N, Hagio M, Ishizuka S, Ito H, Morita T, Sonoyama K. 

Inulin prolongs survival of intragastrically administered Lactobacillus 

plantarum No. 14 in the gut of mice fed a high-fat diet. J Nutr. 2010 

Nov;140(11):1963-9. 

Vlková E, Grmanová M, Killer J, Mrázek J, Kopecný J, Bunesová 

V, Rada V. Survival of bifidobacteria administered to calves. Folia 

Microbiol (Praha). 2010 Jul;55(4):390-2. 

INTERACTION OF PROBIOTICS WITH PATHOGENS 

Belguesmia Y, Choiset Y, Prévost H, Dalgalarrondo M, Chobert JM, 

Drider D. Partial Purification and Characterization of the Mode of 

Action of Enterocin S37: A Bacteriocin Produced by Enterococcus 

faecalis S37 Isolated from Poultry Feces. J Environ Public Health. 

2010;2010. pii: 986460. 

Bøhle LA, Brede DA, Diep DB, Holo H, Nes IF. Specific degradation 

of the mucus adhesion-promoting protein (MapA) of Lactobacillus 

reuteri to an antimicrobial peptide. Appl Environ Microbiol. 2010 

Nov;76(21):7306-9. 

Chiodo PG, Sparo MD, Pezzani BC, Minvielle MC, Basualdo JA. In vitro 

and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara 

canis. Mem Inst Oswaldo Cruz. 2010 Aug;105(5):615-20. 

Dicks LM, Granger M, van Reenen CA. Survival and adherence of 

antimicrobial peptide ST4SA, produced by Enterococcus mundtii, 

at conditions found in the human gastro-intestinal tract. J Basic 

Microbiol. 2010 Oct 21. 

Eom JE, Moon SK, Moon GS. Heterologous production of pediocin 

PA-1 in Lactobacillus reuteri. J Microbiol Biotechnol. 2010 

Aug;20(8):1215-8. 

Gong HS, Meng XC, Wang H. Mode of action of plantaricin MG, a 

bacteriocin active against Salmonella typhimurium. J Basic Microbiol. 

2010 Oct 21. 

Malago JJ, Nemeth E, Koninkx JF, Tooten PC, Fajdiga S, van Dijk JE. 

Microbial products from probiotic bacteria inhibit Salmonella enteritidis 

857-induced IL-8 synthesis in Caco-2 cells. Folia Microbiol (Praha). 

2010 Jul;55(4):401-8. 

Murzyn A, Krasowska A, Augustyniak D, Majkowska-Skrobek G, 

Łukaszewicz M, Dziadkowiec D. The effect of Saccharomyces boulardii 

on Candida albicans-infected human intestinal cell lines Caco-2 and 

Intestin 407. FEMS Microbiol Lett. 2010 Sep 1;310(1):17-23. 

Murzyn A, Krasowska A, Stefanowicz P, Dziadkowiec D, Łukaszewicz 

M. Capric acid secreted by S. boulardii inhibits C. albicans filamentous 

growth, adhesion and biofilm formation. PLoS One. 2010 Aug 

10;5(8):e12050. 

Ruas-Madiedo P, Medrano M, Salazar N, De Los Reyes-Gavilán CG, 

Pérez PF, Abraham AG. Exopolysaccharides produced by Lactobacillus 

and Bifidobacterium strains abrogate in vitro the cytotoxic effect of 

bacterial toxins on eukaryotic cells. J Appl Microbiol. 2010 Aug 19. doi: 

10.1111/j.1365-2672.2010.04839.x. 

Sadowska B, Walencka E, Wieckowska-Szakiel M, Rózalska B. Bacteria 

competing with the adhesion and biofilm formation by Staphylococcus 

aureus. Folia Microbiol (Praha). 2010 Sep;55(5):497-501. 

Smaoui S, Elleuch L, Bejar W, Karray-Rebai I, Ayadi I, Jaouadi B, 

Mathieu F, Chouayekh H, Bejar S, Mellouli L. Inhibition of fungi and 

gram-negative bacteria by bacteriocin BacTN635 produced by 

Lactobacillus plantarum sp. TN635. Appl Biochem Biotechnol. 2010 

Oct;162(4):1132-46. 

22




Contents : 

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THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Stöber H, Maier E, Schmidt H. Protective effects of Lactobacilli, 

Bifidobacteria and Staphylococci on the infection of cultured HT29 

cells with different enterohemorrhagic Escherichia coli serotypes are 

strain-specific. Int J Food Microbiol. 2010 Nov 15;144(1):133-40. 

Todorov SD, Wachsman M, Tomé E, Dousset X, Destro MT, Dicks LM, 

Franco BD, Vaz-Velho M, Drider D. Characterisation of an antiviral 

pediocin-like bacteriocin produced by Enterococcus faecium. Food 

Microbiol. 2010 Oct;27(7):869-79. 

Zihler A, Gagnon M, Chassard C, Hegland A, Stevens MJ, Braegger 

CP, Lacroix C. Unexpected consequences of administering 

bacteriocinogenic probiotic strains for Salmonella populations, 

revealed by an in vitro colonic model of the child gut. Microbiology. 

2010 Nov;156(Pt 11):3342-53. 

Zoumpopoulou G, Papadimitriou K, Polissiou MG, Tarantilis PA, 

Tsakalidou E. Detection of changes in the cellular composition 

of Salmonella enterica serovar Typhimurium in the presence of 

antimicrobial compound(s) of Lactobacillus strains using Fourier 

transform infrared spectroscopy. Int J Food Microbiol. 2010 Nov 

15;144(1):202-7. 

MUCOSA RESPONSE TO PROBIOTICS (TROPHICITY & STRUCTURE) 

Anderson RC, Cookson AL, McNabb WC, Kelly WJ, Roy NC. 

Lactobacillus plantarum DSM 2648 is a potential probiotic that 

enhances intestinal barrier function. FEMS Microbiol Lett. 2010 Aug 

1;309(2):184-92. 

Chen HQ, Yang J, Zhang M, Zhou YK, Shen TY, Chu ZX, Zhang M, 

Hang XM, Jiang YQ, Qin HL. Lactobacillus plantarum ameliorates 

colonic epithelial barrier dysfunction by modulating the apical 

junctional complex and PepT1 in IL-10 knockout mice. Am J Physiol 

Gastrointest Liver Physiol. 2010 Sep 30. 

Generoso SV, Viana ML, Santos RG, Arantes RM, Martins FS, Nicoli JR, 

Machado JA, Correia MI, Cardoso VN. Protection against increased 

intestinal permeability and bacterial translocation induced by intestinal 

obstruction in mice treated with viable and heat-killed Saccharomyces 

boulardii. Eur J Nutr. 2010 Oct 10. 

Zou XP, Chen M, Wei W, Cao J, Chen L, Tian M. Effects of enteral 

immunonutrition on the maintenance of gut barrier function and 

immune function in pigs with severe acute pancreatitis. JPEN J 

Parenter Enteral Nutr. 2010 Sep-Oct;34(5):554-66. 

Van Baarlen P, Troost F, van der Meer C, Hooiveld G, Boekschoten 

M, Brummer RJ, Kleerebezem M. Microbes and Health Sackler 

Colloquium: Human mucosal in vivo transcriptome responses to three 

lactobacilli indicate how probiotics may modulate human cellular 

pathways. Proc Natl Acad Sci U S A. 2010 Sep 7. 

EFFECTS OF PROBIOTICS ON MICROBIOTA 

Brignardello J, Morales P, Diaz E, Romero J, Brunser O, 

Gotteland M. Pilot study: alterations of intestinal microbiota in 

obese humans are not associated with colonic inflammation 

or disturbances of barrier function. Aliment Pharmacol 

Ther. 2010 Dec;32(11-12):1307-14. doi: 10.1111/j.1365- 

2036.2010.04475.x. 

Kong Q, He GQ, Jia JL, Zhu QL, Ruan H. Oral Administration of 

Clostridium butyricum for Modulating Gastrointestinal Microflora in 

Mice. Curr Microbiol. 2010 Aug 15. 

Malinen E, Krogius-Kurikka L, Lyra A, Nikkilä J, Jääskeläinen A, 

Rinttilä T, Vilpponen-Salmela T, von Wright AJ, Palva A. Association of 

symptoms with gastrointestinal microbiota in irritable bowel syndrome. 

World J Gastroenterol. 2010 Sep 28;16(36):4532-40. 

Martin FP, Sprenger N, Montoliu I, Rezzi S, Kochhar S, Nicholson 

JK. Dietary modulation of gut functional ecology studied by faecal 

metabonomics. J Proteome Res. 2010 Oct 1;9(10):5284-95. 

Nakashimada Y, Michinaka A, Watanabe K, Nishio N, Fujii T. Brewer’s 

yeast cell wall affects microbiota composition and decreases 

Bacteroides fragilis populations in an anaerobic gut intestinal model. J 

Biosci Bioeng. 2010 Oct 5. 

Roger LC, Costabile A, Holland DT, Hoyles L, McCartney AL. 

Examination of faecal Bifidobacterium populations in breast- and 

formula-fed infants during the first 18 months of life. Microbiology. 

2010 Nov;156(Pt 11):3329-41. 

Salazar N, Binetti A, Gueimonde M, Alonso A, Garrido P, González 

Del Rey C, González C, Ruas-Madiedo P, de Los Reyes-Gavilán CG. 

Safety and intestinal microbiota modulation by the exopolysaccharideproducing 

strains Bifidobacterium animalis IPLA R1 and 

Bifidobacterium longum IPLA E44 orally administered to Wistar rats. 

Int J Food Microbiol. 2010 Nov 13. 

Samli HE, Dezcan S, Koc F, Ozduven ML, Okur AA, Senkoylu N. 

Effects of Enterococcus faecium supplementation and floor type on 

performance, morphology of erythrocytes and intestinal microbiota in 

broiler chickens. Br Poult Sci. 2010 Aug;51(4):564-8. 

Sánchez E, Donat E, Ribes-Koninckx C, Calabuig M, Sanz Y. 

Intestinal Bacteroides species associated with coeliac disease. 

J Clin Pathol. 2010 Oct 23. 

EFFECTS OF PROBIOTIC ON IMMUNITY (MUCOSAL & SYSTEMIC) 

Borthakur A, Anbazhagan AN, Kumar A, Raheja G, Singh V, Ramaswamy 

K, Dudeja PK. The probiotic Lactobacillus plantarum counteracts TNF- 

{alpha}-induced downregulation of SMCT1 expression and function. 

Am J Physiol Gastrointest Liver Physiol. 2010 Oct;299(4):G928-34. 

Brisbin JT, Gong J, Parvizi P, Sharif S. Effects of lactobacilli on cytokine 

expression by chicken spleen and cecal tonsil cells. Clin Vaccine 

Immunol. 2010 Sep;17(9):1337-43. 

Cazzola M, Tompkins TA, Matera MG. Immunomodulatory impact of a 

synbiotic in T(h)1 and T(h)2 models of infection. Ther Adv Respir Dis. 

2010 Oct;4(5):259-70. 

D’Incà R, Barollo M, Scarpa M, Grillo AR, Brun P, Vettorato 

MG, Castagliuolo I, Sturniolo GC. Rectal Administration of 

Lactobacillus casei DG Modifies Flora Composition and Toll- 

Like Receptor Expression in Colonic Mucosa of Patients with 

Mild Ulcerative Colitis. Dig Dis Sci. 2010 Aug 25. 

Dos Santos LM, Santos MM, de Souza Silva HP, Arantes RM, Nicoli JR, 

Vieira LQ. Monoassociation with probiotic Lactobacillus delbrueckii 

UFV-H2b20 stimulates the immune system and protects germfree 

mice against Listeria monocytogenes infection. Med Microbiol 

Immunol. 2010 Sep 14. 

Edwards LA, Lucas M, Edwards EA, Torrente F, Heuschkel RB, Klein 

NJ, Murch SH, Bajaj-Elliott M, Phillips AD. Aberrant response to 

commensal Bacteroides thetaiotaomicron in Crohn’s disease: An ex 

vivo human organ culture study. Inflamm Bowel Dis. 2010 Nov 4. 

Foligné B, Deutsch SM, Breton J, Cousin FJ, Dewulf J, Samson M, 

Pot B, Jan G. Promising immunomodulatory effects of selected strains 

of dairy propionibacteria evidenced in vitro and in vivo. Appl Environ 

Microbiol. 2010 Oct 22. 

Fredriksen L, Mathiesen G, Sioud M, Eijsink VG. Cell wall anchoring 

of the 37-kilodalton oncofetal antigen by Lactobacillus plantarum 

for mucosal cancer vaccine delivery. Appl Environ Microbiol. 2010 

Nov;76(21):7359-62. 

Grimoud J, Durand H, de Souza S, Monsan P, Ouarné F, Theodorou V, 

Roques C. In vitro screening of probiotics and synbiotics according to 

anti-inflammatory and anti-proliferative effects. Int J Food Microbiol. 

2010 Nov 15;144(1):42-50. 

Gueniche A, Benyacoub J, Philippe D, Bastien P, Kusy N, Breton L, 

Blum S, Castiel-Higounenc I. Lactobacillus paracasei CNCM I-2116 

(ST11) inhibits substance P-induced skin inflammation and accelerates 

skin barrier function recovery in vitro. Eur J Dermatol. 2010 Oct 22. 

Guglielmetti S, Taverniti V, Minuzzo M, Arioli S, Zanoni I, Stuknyte 

M, Granucci F, Karp M, Mora D. A dairy bacterium displays in vitro 

probiotic properties for the pharyngeal mucosa by antagonizing group 

A streptococci and modulating the immune response. Infect Immun. 

2010 Nov;78(11):4734-43. 

Harikrishnan R, Balasundaram C, Heo MS. Effect of probiotics enriched 

diet on Paralichthys olivaceus infected with lymphocystis disease virus 

(LCDV). Fish Shellfish Immunol. 2010 Nov;29(5):868-74. 

Hua MC, Lin TY, Lai MW, Kong MS, Chang HJ, Chen CC. Probiotic 

Bio-Three induces Th1 and anti-inflammatory effects in PBMC and 

dendritic cells. World J Gastroenterol. 2010 Jul 28;16(28):3529-40. 

Igarashi T. Study of the Relationship between Changes in Lactic Acid 

Bacterial Cell Components and Stimulation of IL-12 Production under 

Salt-Stressed Conditions. Biosci Biotechnol Biochem. 2010 Nov 7. 

Jain S, Yadav H, Sinha PR, Kapila S, Naito Y, Marotta F. Anti-allergic 

effects of probiotic Dahi through modulation of the gut immune 

system. Turk J Gastroenterol. 2010 Sep;21(3):244-50. 

Jung BG, Ko JH, Lee BJ. Dietary Supplementation with Probiotic 

Fermented Tetra-Herbal Combination Enhances Immune Activity 

in Broiler Chicks and Increases Survivability against Salmonella 

Gallinarum in Experimentally Infected Broiler Chicks. J Vet Med Sci. 

2010 Jul 28. 

Kato-Mori Y, Orihashi T, Kanai Y, Sato M, Sera K, Hagiwara 

K. Fermentation Metabolites from Lactobacillus gasseri and 

Propionibacterium freudenreichii Exert Bacteriocidal Effects 

in Mice. J Med Food. 2010 Oct 18. 

AD. Lactobacillus rhamnosus GR-1 Stimulates Colony-Stimulating 

Factor 3 (Granulocyte) (CSF3) Output in Placental Trophoblast Cells in 

a Fetal Sex-Dependant Manner. Biol Reprod. 2010 Sep 1. 

23




Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Kotani Y, Shinkai S, Okamatsu H, Toba M, Ogawa K, Yoshida 

H, Fukaya T, Fujiwara Y, Chaves PH, Kakumoto K, Kohda N. 

Oral intake of Lactobacillus pentosus strain b240 accelerates 

salivary immunoglobulin A secretion in the elderly: A 

randomized, placebo-controlled, double-blind trial. Immun 

Ageing. 2010 Aug 26;7:11. 

Lee TY, Kim YH, Lee KS, Kim JK, Lee IH, Yang JM, Sung MH, Park 

JS, Poo H. Human papillomavirus type 16 E6-specific antitumor 

immunity is induced by oral administration of HPV16 E6-expressing 

Lactobacillus casei in C57BL/6 mice. Cancer Immunol Immunother. 

2010 Nov;59(11):1727-37. 

Liu Y, Fatheree NY, Mangalat N, Rhoads JM. Human-derived probiotic 

Lactobacillus reuteri strains differentially reduce intestinal inflammation. 

Am J Physiol Gastrointest Liver Physiol. 2010 Nov;299(5):G1087-96. 

Mair C, Plitzner C, Pfaffl MW, Schedle K, Meyer HH, Windisch W. 

Inulin and probiotics in newly weaned piglets: effects on intestinal 

morphology, mRNA expression levels of inflammatory marker genes 

and haematology. Arch Anim Nutr. 2010 Aug;64(4):304-21. 

Mohamadzadeh M, Durmaz E, Zadeh M, Pakanati KC, Gramarossa M, 

Cohran V, Klaenhammer TR. Targeted expression of anthrax protective 

antigen by Lactobacillus gasseri as an anthrax vaccine. Future 

Microbiol. 2010 Aug;5(8):1289-96. 

Sharifuzzaman SM, Abbass A, Tinsley JW, Austin B. Subcellular 

components of probiotics Kocuria SM1 and Rhodococcus SM2 induce 

protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) 

against Vibrio anguillarum. Fish Shellfish Immunol. 2010 Nov 12. 

Sohail MU, Ijaz A, Yousaf MS, Ashraf K, Zaneb H, Aleem M, Rehman H. 

Alleviation of cyclic heat stress in broilers by dietary supplementation of 

mannan-oligosaccharide and Lactobacillus-based probiotic: dynamics 

of cortisol, thyroid hormones, cholesterol, C-reactive protein, and 

humoral immunity. Poult Sci. 2010 Sep;89(9):1934-8. 

Tao L, Pavlova SI, Ji X, Jin L, Spear G. A novel plasmid for delivering 

genes into mammalian cells with noninvasive food and commensal 

lactic acid bacteria. Plasmid. 2010 Sep 9. 

Tsai YT, Cheng PC, Pan TM. Immunomodulating Activity of Lactobacillus 

paracasei subsp. paracasei NTU 101 in Enterohemorrhagic Escherichia 

coli O157H7-Infected Mice. J Agric Food Chem. 2010 Oct 13. 

Vissers YM, Snel J, Zuurendonk PF, Kleerebezem M, Wichers HJ, 

Savelkoul HF. strains differentially modulate cytokine production by 

hPBMC Lactobacillus from pollen-allergic patients. FEMS Immunol 

Med Microbiol. 2010 Sep 24. doi: 10.1111/j.1574-695X.2010.00741.x. 

Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, 

Kinoshita S, Lee KM, Kawabata M, Shirakawa T. Genetically modified 

Bifidobacterium displaying Salmonella-antigen protects mice from 

lethal challenge of Salmonella Typhimurium in a murine typhoid fever 

model. Vaccine. 2010 Sep 24;28(41):6684-91. 

Yeganegi M, Leung CG, Martins A, Kim SO, Reid G, Challis JR, 

Bocking AD. Lactobacillus rhamnosus GR-1-induced IL-10 production 

in human placental trophoblast cells involves activation of JAK/STAT 

and MAPK pathways. Reprod Sci. 2010 Nov;17(11):1043-51. 

Zhou X, Tian Z, Wang Y, Li W. Effect of treatment with probiotics as 

water additives on tilapia (Oreochromis niloticus) growth performance 

and immune response. Fish Physiol Biochem. 2010 Sep;36(3):501-9. 

OTHER PHYSIOLOGICAL EFFECTS 

Aronsson L, Huang Y, Parini P, Korach-André M, Håkansson J, 

Gustafsson JÅ, Pettersson S, Arulampalam V, Rafter J. Decreased 

fat storage by Lactobacillus paracasei is associated with increased 

levels of angiopoietin-like 4 protein (ANGPTL4). PLoS One. 2010 Sep 

30;5(9). pii: e13087. 

Bloise E, Torricelli M, Novembri R, Borges LE, Carrarelli P, Reis FM, 

Petraglia F. Heat-killed Lactobacillus rhamnosus GG modulates 

urocortin and cytokine release in primary trophoblast cells. Placenta. 

2010 Oct;31(10):867-72. 

Caglar E, Sandalli N, Kuscu OO, Durhan MA, Pisiriciler R, Caliskan 

EA, Kargul B. Viability of fibroblasts in a novel probiotic storage 

media. Dent Traumatol. 2010 Oct;26(5):291-5. doi: 10.1111/j.1600- 

9657.2010.00914.x. 

Capcarova M, Chmelnicna L, Kolesarova A, Massanyi P, Kovacik 

J. Effects of Enterococcus faecium M 74 strain on selected blood 

and production parameters of laying hens. Br Poult Sci. 2010 

Oct;51(5):614-20. 

Chen HQ, Shen TY, Zhou YK, Zhang M, Chu ZX, Hang XM, Qin HL. 

Lactobacillus plantarum Consumption Increases PepT1-Mediated 

Amino Acid Absorption by Enhancing Protein Kinase C Activity in 

Spontaneously Colitic Mice. J Nutr. 2010 Oct 27. 

Di Cagno R, Mazzacane F, Rizzello CG, Vincentini O, Silano M, Giuliani G, De 

Angelis M, Gobbetti M. Synthesis of isoflavone aglycones and equol in soy 

milks fermented by food-related lactic acid bacteria and their effect on human 

intestinal Caco-2 cells. J Agric Food Chem. 2010 Oct 13;58(19):10338-46. 

Fordjour L, D’Souza A, Cai C, Ahmad A, Valencia G, Kumar D, Aranda 

JV, Beharry KD. Comparative effects of probiotics, prebiotics, and 

synbiotics on growth factors in the large bowel in a rat model of 

formula-induced bowel inflammation. J Pediatr Gastroenterol Nutr. 

2010 Oct;51(4):507-13. 

Giorgini E, Conti C, Ferraris P, Sabbatini S, Tosi G, Rubini C, Vaccari 

L, Gioacchini G, Carnevali O. Effects of Lactobacillus rhamnosus 

on zebrafish oocyte maturation: an FTIR imaging and biochemical 

analysis. Anal Bioanal Chem. 2010 Oct 9. 

Kelley R, Soon Park J, O’Mahony L, Minikhiem D, Fix A. Safety and 

Tolerance of Dietary Supplementation With a Canine-Derived Probiotic 

(Bifidobacterium animalis Strain AHC7) Fed to Growing Dogs. Vet Ther. 

2010 Fall;11(3):E1-E14. 

Khailova L, Mount Patrick SK, Arganbright KM, Halpern MD, Kinouchi 

T, Dvorak B. Bifidobacterium bifidum reduces apoptosis in the intestinal 

epithelium in necrotizing enterocolitis. Am J Physiol Gastrointest Liver 

Physiol. 2010 Nov;299(5):G1118-27. 

Kim Y, Oh S, Yun HS, Oh S, Kim SH. Cell-bound exopolysaccharide 

from probiotic bacteria induces autophagic cell death of tumour cells. 

Lett Appl Microbiol. 2010 Aug;51(2):123-30. 

Kumar R, Grover S, Batish VK. Hypocholesterolaemic effect of dietary 

inclusion of two putative probiotic bile salt hydrolase-producing 

Lactobacillus plantarum strains in Sprague-Dawley rats. Br J Nutr. 

2010 Oct 6:1-12. 

Lieske JC, Tremaine WJ, De Simone C, O’Connor HM, Li X, 

Bergstralh EJ, Goldfarb DS. Diet, but not oral probiotics, 

effectively reduces urinary oxalate excretion and calcium 

oxalate supersaturation. Kidney Int. 2010 Aug 25. 

Lin YW, Mou YC, Su CC, Chiang BH. Antihepatocarcinoma activity of 

lactic acid bacteria fermented Panax notoginseng. J Agric Food Chem. 

2010 Aug 11;58(15):8528-34. 

Ma EL, Choi YJ, Choi J, Pothoulakis C, Rhee SH, Im E. The anticancer 

effect of probiotic Bacillus polyfermenticus on human colon cancer 

cells is mediated through ErbB2 and ErbB3 inhibition. Int J Cancer. 

2010 Aug 15;127(4):780-90. 

Ooi LG, Ahmad R, Yuen KH, Liong MT. Lactobacillus acidophilus 

CHO-220 and inulin reduced plasma total cholesterol and lowdensity 

lipoprotein cholesterol via alteration of lipid transporters. 

J Dairy Sci. 2010 Nov;93(11):5048-58.. 

Ooi LG, Bhat R, Rosma A, Yuen KH, Liong MT. A synbiotic 

containing Lactobacillus acidophilus CHO-220 and inulin 

improves irregularity of red blood cells. J Dairy Sci. 2010 

Oct;93(10):4535-44. 

Osman A, Tzortzis G, Rastall RA, Charalampopoulos D. A comprehensive 

investigation of the synthesis of prebiotic galactooligosaccharides by 

whole cells of Bifidobacterium bifidum NCIMB 41171. J Biotechnol. 

2010 Oct 1;150(1):140-8. 

Pan DD, Zeng XQ, Yan YT. Characterisation of Lactobacillus fermentum 

SM-7 isolated from koumiss, a potential probiotic bacterium with 

cholesterol-lowering effects. J Sci Food Agric. 2010 Oct 28. 

Sazawal S, Dhingra U, Hiremath G, Sarkar A, Dhingra P, Dutta 

A, Menon VP, Black RE. Effects of Bifidobacterium lactis 

HN019 and prebiotic oligosaccharide added to milk on iron 

status, anemia, and growth among children 1 to 4 years old. J 

Pediatr Gastroenterol Nutr. 2010 Sep;51(3):341-6. 

Sengül N, Isık S, Aslım B, Uçar G, Demirbag AE. The Effect of 

Exopolysaccharide-Producing Probiotic Strains on Gut Oxidative 

Damage in Experimental Colitis. Dig Dis Sci. 2010 Aug 4. 

Shen Q, Zhang B, Xu R, Wang Y, Ding X, Li P. Antioxidant activity 

in vitro of the selenium-contained protein from the Se-enriched 

Bifidobacterium animalis 01. Anaerobe. 2010 Aug;16(4):380-6. 

Shin HS, Park SY, Lee do K, Kim SA, An HM, Kim JR, Kim MJ, Cha 

MG, Lee SW, Kim KJ, Lee KO, Ha NJ. Hypocholesterolemic effect 

of sonication-killed Bifidobacterium longum isolated from healthy 

adult Koreans in high cholesterol fed rats. Arch Pharm Res. 2010 

Sep;33(9):1425-31. 

Shirasawa Y, Shibahara-Sone H, Iino T, Ishikawa F. Bifidobacterium 

bifidum BF-1 suppresses Helicobacter pylori-induced genes in human 

epithelial cells. J Dairy Sci. 2010 Oct;93(10):4526-34. 

Truusalu K, Kullisaar T, Hütt P, Mahlapuu R, Aunapuu M, Arend A, 

Zilmer M, Mikelsaar RH, Mikelsaar M. Immunological, antioxidative, 

and morphological response in combined treatment of ofloxacin and 

Lactobacillus fermentum ME-3 probiotic in Salmonella Typhimurium 

murine model. APMIS. 2010 Nov;118(11):864-72. doi: 10.1111/j.1600- 

0463.2010.02672.x. 

Wind RD, Tolboom H, Klare I, Huys G, Knol J. Tolerance 

and safety of the potentially probiotic strain Lactobacillus 

rhamnosus PRSF-L477: a randomised, double-blind placebocontrolled 

trial in healthy volunteers. Br J Nutr. 2010 Aug 

9:1-11. 

24




Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Wu MH, Pan TM, Wu YJ, Chang SJ, Chang MS, Hu CY. Exopolysaccharide 

activities from probiotic bifidobacterium: Immunomodulatory effects 

(on J774A.1 macrophages) and antimicrobial properties. Int J Food 

Microbiol. 2010 Nov 15;144(1):104-10. 

Wutzke KD, Lotz M, Zipprich C. The effect of pre- and 

probiotics on the colonic ammonia metabolism in humans as 

measured by lactose-[(15)N(2)]ureide. Eur J Clin Nutr. 2010 

Oct;64(10):1215-21. 

Yeganegi M, Leung CG, Martins A, Kim SO, Reid G, Challis JR, Bocking 

AD. Lactobacillus rhamnosus GR-1 Stimulates Colony-Stimulating 

Factor 3 (Granulocyte) (CSF3) Output in Placental Trophoblast Cells in 

a Fetal Sex-Dependant Manner. Biol Reprod. 2010 Sep 1. 

IN VIVO OUTCOMES OF PROBIOTICS INTAKE 

INFECTIONS (GI, RESPIRATORY, GENITO-URINARY, OTHER) 

Asahara T, Shimizu K, Takada T, Kado S, Yuki N, Morotomi M, Tanaka 

R, Nomoto K. Protective effect of Lactobacillus casei strain Shirota 

against lethal infection with multi-drug resistant Salmonella enterica 

serovar Typhimurium DT104 in mice. J Appl Microbiol. 2010 Oct 15. 

doi: 10.1111/j.1365-2672.2010.04884.x. 

Berggren A, Lazou Ahrén I, Larsson N, Onning G. Randomised, 

double-blind and placebo-controlled study using new probiotic 

lactobacilli for strengthening the body immune defence against 

viral infections. Eur J Nutr. 2010 Aug 28. 

Braga TD, da Silva GA, de Lira PI, de Carvalho Lima M. 

Efficacy of Bifidobacterium breve and Lactobacillus casei oral 

supplementation on necrotizing enterocolitis in very-low-birthweight 

preterm infants: a double-blind, randomized, controlled 

trial. Am J Clin Nutr. 2010 Oct 27. 

Cazzola M, Pham-Thi N, Kerihuel JC, Durand H, Bohbot S. 

Efficacy of a synbiotic supplementation in the prevention of 

common winter diseases in children: a randomized, doubleblind, 

placebo-controlled pilot study. Ther Adv Respir Dis. 2010 

Oct;4(5):271-8. 

Chiu CH, Cheng CH, Gua WR, Guu YK, Cheng W. Dietary administration 

of the probiotic, Saccharomyces cerevisiae P13, enhanced the growth, 

innate immune responses, and disease resistance of the grouper, 

Epinephelus coioides. Fish Shellfish Immunol. 2010 Dec;29(6):1053-9. 

Donders GG, Van Bulck B, Van de Walle P, Kaiser RR, Pohlig G, 

Gonser S, Graf F. Effect of Lyophilized Lactobacilli and 0.03 mg 

Estriol (Gynoflor®) on Vaginitis and Vaginosis with Disrupted 

Vaginal Microflora: A Multicenter, Randomized, Single-Blind, 

Active-Controlled Pilot Study. Gynecol Obstet Invest. 2010 Oct 

16;70(4):264-272. 

Eaton KA, Honkala A, Auchtung TA, Britton RA. Probiotic Lactobacillus 

reuteri ameliorates disease due to Enterohemorrhagic Escherichia coli 

in germ free mice. Infect Immun. 2010 Oct 25. 

Ferrie S, Daley M. Lactobacillus GG as Treatment for Diarrhea 

During Enteral Feeding in Critical Illness: Randomized 

Controlled Trial. JPEN J Parenter Enteral Nutr. 2010 Oct 26. 

Geier MS, Mikkelsen LL, Torok VA, Allison GE, Olnood CG, Boulianne 

M, Hughes RJ, Choct M. Comparison of alternatives to in-feed 

antimicrobials for the prevention of clinical necrotic enteritis. J 

Appl Microbiol. 2010 Oct;109(4):1329-1338. doi: 10.1111/j.1365- 

2672.2010.04758.x. 

Grandy G, Medina M, Soria R, Terán CG, Araya M. Probiotics 

in the treatment of acute rotavirus diarrhoea. A randomized, 

double-blind, controlled trial using two different probiotic 

preparations in Bolivian children. BMC Infect Dis. 2010 Aug 

25;10:253. 

Guthmann F, Kluthe C, Bührer C. Probiotics for prevention of 

necrotising enterocolitis: an updated meta-analysis. Klin Padiatr. 2010 

Sep;222(5):284-90. 

Harikrishnan R, Balasundaram C, Heo MS. Lactobacillus sakei BK19 

enriched diet enhances the immunity status and disease resistance to 

streptococcosis infection in kelp grouper, Epinephelus bruneus. Fish 

Shellfish Immunol. 2010 Dec;29(6):1037-43. 

Krause DO, Bhandari SK, House JD, Nyachoti CM. Response of nursery 

pigs to a synbiotic preparation of starch and an anti-Escherichia coli 

K88 probiotic. Appl Environ Microbiol. 2010 Oct 15. 

Oudhuis GJ, Bergmans DC, Dormans T, Zwaveling JH, Kessels 

A, Prins MH, Stobberingh EE, Verbon A. Probiotics versus 

antibiotic decontamination of the digestive tract: infection and 

mortality. Intensive Care Med. 2010 Aug 19. 

Sazawal S, Dhingra U, Hiremath G, Sarkar A, Dhingra P, Dutta A, 

Verma P, Menon VP, Black RE. Prebiotic and probiotic fortified 

milk in prevention of morbidities among children: communitybased, 

randomized, double-blind, controlled trial. PLoS One. 

2010 Aug 13;5(8):e12164. 

Sur D, Manna B, Niyogi SK, Ramamurthy T, Palit A, Nomoto 

K, Takahashi T, Shima T, Tsuji H, Kurakawa T, Takeda Y, Nair 

GB, Bhattacharya SK. Role of probiotic in preventing acute 

diarrhoea in children: a community-based, randomized, doubleblind 

placebo-controlled field trial in an urban slum. Epidemiol 

Infect. 2010 Jul 30:1-8. 

Taipale T, Pienihäkkinen K, Isolauri E, Larsen C, Brockmann 

E, Alanen P, Jokela J, Söderling E. Bifidobacterium animalis 

subsp. lactis BB-12 in reducing the risk of infections in infancy. 

Br J Nutr. 2010 Sep 24:1-7. 

Ya W, Reifer C, Miller LE. Efficacy of vaginal probiotic capsules 

for recurrent bacterial vaginosis: a double-blind, randomized, 

placebo-controlled study. Am J Obstet Gynecol. 2010 

Aug;203(2):120.e1-6. 

Yasar B, Abut E, Kayadıbı H, Toros B, Sezıklı M, Akkan 

Z, Keskın Ö, Övünç Kurdas O. Efficacy of probiotics in 

Helicobacter pylori eradication therapy. Turk J Gastroenterol. 

2010 Sep;21(3):212-7. 

ALLERGY & ATOPY 

Huang J, Zhong Y, Cai W, Zhang H, Tang W, Chen B. The effects of 

probiotics supplementation timing on an ovalbumin-sensitized rat 

model. FEMS Immunol Med Microbiol. 2010 Nov;60(2):132-41. doi: 

10.1111/j.1574-695X.2010.00727.x. 

Koyama T, Kirjavainen PV, Fisher C, Anukam K, Summers K, 

Hekmat S, Reid G. Development and pilot evaluation of a novel 

probiotic mixture for the management of seasonal allergic 

rhinitis. Can J Microbiol. 2010 Sep;56(9):730-8. 

Nagata Y, Yoshida M, Kitazawa H, Araki E, Gomyo T. 

Improvements in seasonal allergic disease with Lactobacillus 

plantarum No. 14. Biosci Biotechnol Biochem. 2010 Sep 

23;74(9):1869-77. 

Penders J, Thijs C, Mommers M, Stobberingh EE, Dompeling 

E, Reijmerink NE, van den Brandt PA, Kerkhof M, Koppelman 

GH, Postma DS. Intestinal lactobacilli and the DC-SIGN gene 

for their recognition by dendritic cells play a role in the aetiology 

of allergic manifestations. Microbiology. 2010 Nov;156(Pt 

11):3298-305. 

Rose MA, Stieglitz F, Köksal A, Schubert R, Schulze J, 

Zielen S. Efficacy of probiotic Lactobacillus GG on allergic 

sensitization and asthma in infants at risk. Clin Exp Allergy. 

2010 Sep;40(9):1398-405. 

Schiavi E, Barletta B, Butteroni C, Corinti S, Boirivant M, Di Felice 

G. Oral therapeutic administration of a probiotic mixture suppresses 

established Th2 responses and systemic anaphylaxis in a murine 

model of food allergy. Allergy. 2010 Nov 8. doi: 10.1111/j.1398- 

9995.2010.02501.x. 

Schwarzer M, Repa A, Daniel C, Schabussova I, Hrncir T, Pot B, 

Stepankova R, Hudcovic T, Pollak A, Tlaskalova-Hogenova H, 

Wiedermann U, Kozakova H. Neonatal colonization of mice with 

Lactobacillus plantarum producing the aeroallergen Bet v 1 biases 

towards Th1 and T-regulatory responses upon systemic sensitization. 

Allergy. 2010 Sep 29. doi: 10.1111/j.1398-9995.2010.02488.x. 

Shah MM, Miyamoto Y, Yamada Y, Yamashita H, Tanaka H, Ezaki T, 

Nagai H, Inagaki N. Orally supplemented Lactobacillus acidophilus 

strain L-92 inhibits passive and active cutaneous anaphylaxis as 

well as 2,4-dinitroflurobenzene and mite faecal antigen induced 

atopic dermatitis-like skin lesions in mice. Microbiol Immunol. 2010 

Sep;54(9):523-33. 

Torii S, Torii A, Itoh K, Urisu A, Terada A, Fujisawa T, Yamada K, 

Suzuki H, Ishida Y, Nakamura F, Kanzato H, Sawada D, Nonaka 

A, Hatanaka M, Fujiwara S. Effects of Oral Administration of 

Lactobacillus acidophilus L-92 on the Symptoms and Serum 

Markers of Atopic Dermatitis in Children. Int Arch Allergy 

Immunol. 2010 Sep 21;154(3):236-245. 

Van de Pol MA, Lutter R, Smids BS, Weersink EJ, van der Zee 

JS. Synbiotics reduce allergen-induced T-helper 2 response 

and improve peak expiratory flow in allergic asthmatics. 

Allergy. 2010 Aug 17. 

IBS & FUNCTIONAL GI DISORDERS 

Del Piano M, Carmagnola S, Anderloni A, Andorno S, Ballarè 

M, Balzarini M, Montino F, Orsello M, Pagliarulo M, Sartori M, 

Tari R, Sforza F, Capurso L. The use of probiotics in healthy 

volunteers with evacuation disorders and hard stools: a 

double-blind, randomized, placebo-controlled study. J Clin 

Gastroenterol. 2010 Sep;44 Suppl 1:S30-4. 

Francavilla R, Miniello V, Magistà AM, De Canio A, Bucci N, 

Gagliardi F, Lionetti E, Castellaneta S, Polimeno L, Peccarisi 

L, Indrio F, Cavallo L. A Randomized Controlled Trial of 

Lactobacillus GG in Children With Functional Abdominal Pain. 

Pediatrics. 2010 Nov 15. 

25




Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Jeon JR, Choi JH. Lactic acid fermentation of germinated barley fiber 

and proliferative function of colonic epithelial cells in loperamideinduced 

rats. J Med Food. 2010 Aug;13(4):950-60. 

Lee H, Kim YH, Kim JH, Chang DK, Kim JY, Son HJ, Rhee PL, 

Kim JJ, Rhee JC. A feasibility study of probiotics pretreatment 

as a bowel preparation for colonoscopy in constipated 

patients. Dig Dis Sci. 2010 Aug;55(8):2344-51. 

Lyra A, Krogius-Kurikka L, Nikkilä J, Malinen E, Kajander 

K, Kurikka K, Korpela R, Palva A. Effect of a multispecies 

probiotic supplement on quantity of irritable bowel syndromerelated 

intestinal microbial phylotypes. BMC Gastroenterol. 

2010 Sep 19;10:110. 

Nakabayashi I, Nakamura M, Kawakami K, Ohta T, Kato I, 

Uchida K, Yoshida M. Effects of synbiotic treatment on serum 

level of p-cresol in haemodialysis patients: a preliminary study. 

Nephrol Dial Transplant. 2010 Oct 7. 

IBD AND OTHER INFLAMMATORY DISEASES 

Amdekar S, Singh V, Singh R, Sharma P, Keshav P, Kumar A. 

Lactobacillus casei reduces the Inflammatory Joint Damage 

Associated with Collagen-Induced Arthritis (CIA) by Reducing the Pro- 

Inflammatory Cytokines : Lactobacillus casei: COX-2 inhibitor. J Clin 

Immunol. 2010 Sep 14. 

Chu ZX, Chen HQ, Ma YL, Zhou YK, Zhang M, Zhang P, Qin HL. 

Lactobacillus plantarum prevents the upregulation of adhesion 

molecule expression in an experimental colitis model. Dig Dis Sci. 

2010 Sep;55(9):2505-13. 

Claes IJ, Lebeer S, Shen C, Verhoeven TL, Dilissen E, De Hertogh 

G, Bullens DM, Ceuppens JL, Van Assche G, Vermeire S, Rutgeerts 

P, Vanderleyden J, De Keersmaecker SC. Impact of lipoteichoic 

acid modification on the performance of the probiotic Lactobacillus 

rhamnosus GG in experimental colitis. Clin Exp Immunol. 2010 

Nov;162(2):306-14. doi: 10.1111/j.1365-2249.2010.04228.x. Epub 

2010 Aug 20. 

Grijó NN, Borra RC, Sdepanian VL. Proinflammatory and antiinflammatory 

cytokines present in the acute phase of experimental 

colitis treated with Saccharomyces boulardii. Dig Dis Sci. 2010 

Sep;55(9):2498-504. 

Hegazy SK, El-Bedewy MM. Effect of probiotics on proinflammatory 

cytokines and NF-kappaB activation in ulcerative 

colitis. World J Gastroenterol. 2010 Sep 7;16(33):4145-51. 

Mondot S, Kang S, Furet JP, Aguirre de Carcer D, McSweeney 

C, Morrison M, Marteau P, Doré J, Leclerc M. Highlighting new 

phylogenetic specificities of Crohn’s disease microbiota. Inflamm 

Bowel Dis. 2010 Aug 18. 

Savino F, Cordisco L, Tarasco V, Palumeri E, Calabrese R, Oggero R, 

Roos S, Matteuzzi D. Lactobacillus reuteri DSM 17938 in infantile colic: 

a randomized, double-blind, placebo-controlled trial. Pediatrics. 2010 

Sep;126(3):e526-33. 

Steed H, Macfarlane GT, Blackett KL, Bahrami B, Reynolds 

N, Walsh SV, Cummings JH, Macfarlane S. Clinical trial: 

the microbiological and immunological effects of synbiotic 

consumption - a randomized double-blind placebo-controlled 

study in active Crohn’s disease. Aliment Pharmacol Ther. 2010 

Oct;32(7):872-83. doi: 10.1111/j.1365-2036.2010.04417.x. 

Veiga P, Gallini CA, Beal C, Michaud M, Delaney ML, DuBois 

A, Khlebnikov A, van Hylckama Vlieg JE, Punit S, Glickman JN, 

Onderdonk A, Glimcher LH, Garrett WS. Bifidobacterium animalis 

subsp. lactis fermented milk product reduces inflammation by altering 

a niche for colitogenic microbes. Proc Natl Acad Sci U S A. 2010 Oct 

19;107(42):18132-7. 

Watterlot L, Rochat T, Sokol H, Cherbuy C, Bouloufa I, Lefèvre F, 

Gratadoux JJ, Honvo-Hueto E, Chilmonczyk S, Blugeon S, Corthier 

G, Langella P, Bermúdez-Humarán LG. Intragastric administration of 

a superoxide dismutase-producing recombinant Lactobacillus casei 

BL23 strain attenuates DSS colitis in mice. Int J Food Microbiol. 2010 

Nov 15;144(1):35-41. 

NUTRITION: OBESITY, CHOLESTEROL… 

Aaltonen J, Ojala T, Laitinen K, Poussa T, Ozanne S, Isolauri E. 

Impact of maternal diet during pregnancy and breastfeeding 

on infant metabolic programming: a prospective randomized 

controlled study. Eur J Clin Nutr. 2010 Oct 13. 

Awad WA, Ghareeb K, Böhm J. Effect of addition of a probiotic 

micro-organism to broiler diet on intestinal mucosal architecture and 

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Aug 1;94(4):486-94. 

Capcarova M, Hascik P, Kolesarova A, Kacaniova M, Mihok 

M, Pal G. The effect of selected microbial strains on internal 

milieu of broiler chickens after peroral administration. Res Vet 

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Collado MC, Isolauri E, Laitinen K, Salminen S. Effect 

of mother’s weight on infant’s microbiota acquisition, 

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2010 Nov;92(5):1023-30. 

Gebru E, Lee JS, Son JC, Yang SY, Shin SA, Kim B, Kim MK, Park SC. 

Effect of probiotic-, bacteriophage-, or organic acid-supplemented 

feeds or fermented soybean meal on the growth performance, acutephase 

response, and bacterial shedding of grower pigs challenged with 

Salmonella enterica serotype Typhimurium. J Anim Sci. 2010 Aug 20. 

Ilmonen J, Isolauri E, Poussa T, Laitinen K. Impact of 

dietary counselling and probiotic intervention on maternal 

anthropometric measurements during and after pregnancy: A 

randomized placebo-controlled trial. Clin Nutr. 2010 Oct 21. 

Kang JH, Yun SI, Park HO. Effects of Lactobacillus gasseri BNR17 on 

body weight and adipose tissue mass in diet-induced overweight rats. 

J Microbiol. 2010 Oct;48(5):712-4. 

Kondo S, Xiao JZ, Satoh T, Odamaki T, Takahashi S, Sugahara 

H, Yaeshima T, Iwatsuki K, Kamei A, Abe K. Antiobesity effects 

of bifidobacterium breve strain B-3 supplementation in a mouse 

model with high-fat diet-induced obesity. Biosci Biotechnol 

Biochem. 2010 Aug 23;74(8):1656-61. 

Sefcíková Z, Kmet V, Bujnáková D, Racek L, Mozes S. Development 

of gut microflora in obese and lean rats. Folia Microbiol (Praha). 2010 

Jul;55(4):373-5. 

Taheri HR, Moravej H, Tabandeh F, Zaghari M, Shivazad M. Efficacy of 

combined or single use of Lactobacillus crispatus LT116 and L. johnsonii 

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NEW APPLICATION & OTHER 

Andreasen AS, Larsen N, Pedersen-Skovsgaard T, Berg RM, 

Møller K, Svendsen KD, Jakobsen M, Pedersen BK. Effects of 

Lactobacillus acidophilus NCFM on insulin sensitivity and the 

systemic inflammatory response in human subjects. Br J Nutr. 

2010 Sep 6:1-8. 

Castro MS, Molina MA, Di Sciullo P, Azpiroz MB, Leocata Nieto F, 

Sterín-Speziale NB, Mongini C, Manghi MA. Beneficial activity of 

Enterococcus faecalis CECT7121 in the anti-lymphoma protective 

response. J Appl Microbiol. 2010 Oct;109(4):1234-43. doi: 

10.1111/j.1365-2672.2010.04747.x. 

Desbonnet L, Garrett L, Clarke G, Kiely B, Cryan JF, Dinan TG. Effects 

of the probiotic Bifidobacterium infantis in the maternal separation 

model of depression. Neuroscience. 2010 Nov 10;170(4):1179-88. 

Doi A, Nakajo K, Kamiya T, Ohkusu K. Splenic abscess caused 

by Lactobacillus paracasei. J Infect Chemother. 2010 Jul 29. 

Gioacchini G, Maradonna F, Lombardo F, Bizzaro D, Olivotto I, Carnevali 

O. Increase of fecundity by probiotic administration in zebrafish (Danio 

rerio). Reproduction. 2010 Sep 10. 

Hammad AM, Shimamoto T. Towards a compatible probiotic-antibiotic 

combination therapy: assessment of antimicrobial resistance in the 

Japanese probiotics. J Appl Microbiol. 2010 Oct;109(4):1349-60. doi: 

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of probiotic Lactobacillus salivarius WB21 on halitosis and oral 

health: an open-label pilot trial. Oral Surg Oral Med Oral Pathol 

Oral Radiol Endod. 2010 Aug;110(2):201-8. 

Jenks K, Stebbings S, Burton J, Schultz M, Herbison 

P, Highton J. Probiotic therapy for the treatment of 

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2010 Oct;37(10):2118-25. 

Lee KC, Liu CF, Lin TH, Pan TM. Safety and risk assessment of the 

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Food Microbiol. 2010 Aug 15;142(1-2):164-9. 

Liu JE, Zhang Y, Zhang J, Dong PL, Chen M, Duan ZP. Probiotic 

yogurt effects on intestinal flora of patients with chronic liver 

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Lu YC, Yin LT, Chang WT, Huang JS. Effect of Lactobacillus reuteri 

GMNL-263 treatment on renal fibrosis in diabetic rats. J Biosci Bioeng. 

2010 Aug 4. 

Luong ML, Sareyyupoglu B, Nguyen MH, Silveira FP, Shields RK, 

Potoski BA, Pasculle WA, Clancy CJ, Toyoda Y. Lactobacillus 

probiotic use in cardiothoracic transplant recipients: a link to 

invasive Lactobacillus infection? Transpl Infect Dis. 2010 Oct 

7. doi: 10.1111/j.1399-3062.2010.00580.x. 

Mayrhofer S, van Hoek AH, Mair C, Huys G, Aarts HJ, Kneifel W, 

Domig KJ. Antibiotic susceptibility of members of the Lactobacillus 

acidophilus group using broth microdilution and molecular 

identification of their resistance determinants. Int J Food Microbiol. 

2010 Nov 15;144(1):81-7. 

26




Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Messaoudi M, Lalonde R, Violle N, Javelot H, Desor D, 

Nejdi A, Bisson JF, Rougeot C, Pichelin M, Cazaubiel M, 

Cazaubiel JM. Assessment of psychotropic-like properties of 

a probiotic formulation ( Lactobacillus helveticus R0052 and 

Bifidobacterium longum R0175) in rats and human subjects. 

Br J Nutr. 2010 Oct 26:1-9. 

Munakata S, Arakawa C, Kohira R, Fujita Y, Fuchigami 

T, Mugishima H. A case of D-lactic acid encephalopathy 

associated with use of probiotics. Brain Dev. 2010 

Sep;32(8):691-4. 

Myhre R, Brantsæter AL, Myking S, Gjessing HK, Sengpiel V, 

Meltzer HM, Haugen M, Jacobsson B. Intake of probiotic food 

and risk of spontaneous preterm delivery. Am J Clin Nutr. 2010 

Oct 27. 

Qiao GH, Shan AS, Ma N, Ma QQ, Sun ZW. Effect of supplemental 

Bacillus cultures on rumen fermentation and milk yield in Chinese 

Holstein cows. J Anim Physiol Anim Nutr (Berl). 2010 Aug 

1;94(4):429-36. 

Ranganathan N, Ranganathan P, Friedman EA, Joseph A, 

Delano B, Goldfarb DS, Tam P, Rao AV, Anteyi E, Musso CG. 

Pilot study of probiotic dietary supplementation for promoting 

healthy kidney function in patients with chronic kidney disease. 

Adv Ther. 2010 Sep;27(9):634-47. 

Robin F, Paillard C, Marchandin H, Demeocq F, Bonnet R, 

Hennequin C. Lactobacillus rhamnosus meningitis following 

recurrent episodes of bacteremia in a child undergoing 

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Szabo NJ, Dolan LC, Burdock GA, Shibano T, Sato SI, Suzuki 

H, Uesugi T, Yamahira S, Toba M, Ueno H. Safety evaluation of 

Lactobacillus pentosus strain b240. Food Chem Toxicol. 2010 

Nov 5. 

Uchida M, Shimizu K, Kurakazu K. Yogurt containing Lactobacillus 

gasseri OLL 2716 (LG21 yogurt) accelerated the healing of acetic acidinduced 

gastric ulcer in rats. Biosci Biotechnol Biochem. 2010 Sep 

23;74(9):1891-4. 

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Survey. J Clin Gastroenterol. 2010 Sep 8. 

Zhang MM, Cheng JQ, Lu YR, Yi ZH, Yang P, Wu XT. Use of pre-, 

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World J Gastroenterol. 2010 Aug 21;16(31):3970-8. 

REVIEWS 

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10;11:CD003048.. 

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Oct;93(10):4471-9. 

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Saudi Med J. 2010 Sep;31(9):1071-2; author reply 1071-2. 

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Bernaola Aponte G, Bada Mancilla CA, Carreazo Pariasca NY, Rojas 

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Capurso L, Morelli L. Probiotics and European Food Safety Authority 

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Coudeyras S, Forestier C. Microbiote et probiotiques : impact en sante 

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Daniels C, Güroy D, Davies SJ. Microbial manipulations to improve fish 

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Fölster-Holst R. Probiotics in the treatment and prevention of atopic 

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Foster KJ, Lin S, Turck CJ. Current and emerging strategies for 

treating hepatic encephalopathy. Crit Care Nurs Clin North Am. 2010 

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Friswell M, Campbell B, Rhodes J. The role of bacteria in the 

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Gastroenterol Clin Biol. 2010 Sep;34 Suppl 1:S37-43. 

Guéniche A, Bastien P, Ovigne JM, Kermici M, Courchay G, Chevalier 

V, Breton L, Castiel-Higounenc I. Bifidobacterium longum lysate, a new 

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Hummelen R, Vos AP, van’t Land B, van Norren K, Reid G. Altered 

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27




Contents : 

EDITORIAL 


THE QUARTER 






HOST 



REFERENCES 

KEY DATES 

Jack L, Coyer F, Courtney M, Venkatesh B. Probiotics and diarrhoea 

management in enterally tube fed critically ill patients-What is the 

evidence? Intensive Crit Care Nurs. 2010 Aug 6. 

Kachaamy T, Bajaj JS. Diet and cognition in chronic liver disease. Curr 

Opin Gastroenterol. 2010 Oct 21. 

Klein G. Antibiotic Resistance and Molecular Characterization of 

Probiotic and Clinical Lactobacillus Strains in Relation to Safety 

Aspects of Probiotics. Foodborne Pathog Dis. 2010 Oct 29. 

Koteyko N. Balancing the good, the bad and the better: a discursive 

perspective on probiotics and healthy eating. Health (London). 2010 

Nov;14(6):585-602.. 

Lakhan SE, Kirchgessner A. Gut inflammation in chronic fatigue 

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Lee JH, O’Sullivan DJ. Genomic insights into bifidobacteria. Microbiol 

Mol Biol Rev. 2010 Sep;74(3):378-416. 

Marteau P. [The clinical importance of intestinal microbiota]. 

Gastroenterol Clin Biol. 2010 Sep;34 Suppl 1:S93-7. 

McFarland LV. Lactobacillus GG prevented nosocomial gastrointestinal 

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Oct 19. 

McFarland LV. Probiotics and diarrhea. Ann Nutr Metab. 2010;57 

Suppl:10-1. 

Meier R. Probiotics in irritable bowel syndrome. Ann Nutr Metab. 

2010;57 Suppl:12-3. 

Meuer S. Probiotics and respiratory tract diseases. Ann Nutr Metab. 

2010;57 Suppl:24-6. 

Millar M, Wilks M, Fleming P, Costeloe K. Should the use of probiotics 

in the preterm be routine? Arch Dis Child Fetal Neonatal Ed. 2010 

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of Minimal Hepatic Encephalopathy. Curr Gastroenterol Rep. 2010 Oct 6. 

Moore SR. Update on prolonged and persistent diarrhea in children. 

Curr Opin Gastroenterol. 2010 Sep 10. 

Musso G, Gambino R, Cassader M. Obesity, diabetes, and gut 

microbiota: the hygiene hypothesis expanded? Diabetes Care. 2010 

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Payne D. Sticky labels. BMJ. 2010 Oct 26;341:c5861. doi: 10.1136/ 

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Pham LC, Hoogenkamp MA, Exterkate RA, Terefework Z, de Soet JJ, 

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Prado S, Romalde JL, Barja JL. Review of probiotics for use in bivalve 

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Preidis GA, Hill C, Guerrant RL, Ramakrishna BS, Tannock GW, 

Versalovic J. Probiotics, Enteric and Diarrheal Diseases, and Global 

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Roberfroid M, Gibson GR, Hoyles L, McCartney AL, Rastall R, Rowland 

I, Wolvers D, Watzl B, Szajewska H, Stahl B, Guarner F, Respondek F, 

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2010 Sep 21. 

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Schölmerich J. Evolving concepts in IBD therapy: what should be 

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Schwartz M, Regueiro M. Prevention and Treatment of Postoperative 

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28

probiotics 

watch 

issUE N°4 

KEY DATES 

International Human 

Microbiome Congress 

METHOD 

> Monitoring period : 28/07/2010 to 15/11/2010 

> Database : Medline 

> Result : 371 publications 

THE EDITORIAL COMMITTE 

February 26 

March 1 

March 9-11 

May 7-10 

May 13-17 

May 20-24 

> Keywords : probiotic / lactic acid bacteria / 

streptococcus thermophilus / lactobacilli / fermented 

milk / bifidobacteria 

this probiotics watch is designed as a time-saving tool for scientists and clinicians interested in probiotic research. 

in an interactive format, the quarterly report provides timely, quasi-exhaustive lists of the scientific publications of 

the previous three months. it sorts them by topic and highlights some of the most relevant results. readers can 

also check out upcoming scientific events and regular bibliometric analyses. 

objectivity is a strong commitment, that’s why the articles are selected by an editorial committee, composed of 

renowned scientists in the field. Editorial committee members also comment on what they believe are the quarter’s 

most relevant publications. 

Last but not least each issue features an editorial by a probiotic expert, which offers special insight into this 

fascinating field of science. 

Bruno Pot, 

Institut Pasteur de Lille, FRANCE. 

Hania Szajewska, 

The Medical University of Warsaw, Warsaw, POLAND. 

James Versalovic, 

Baylor College of Medicine, Houston, Texas, USA. 

April 14-16 

The members : Its missions : 

Vancouver, Canada 

http://www.cag-acg.org 

Vancouver, Canada 

http://www.cvent.com/EVENTS/Info/ 

Summary.aspx?e=c4aa192c-e47c-44fbb1dc-15dd34e5b2c2 

Chicago, USA 

http://www.ddw.org 

San Francisco, USA 

http://www.immunology2011.org 

Washington, USA 

http://gm.asm.org/ 

Providing international and multidisciplinary 

vision. 

Selecting the articles of interest in the 

probiotics field. 

Commenting the most relevant publications. 

HEALTH AFFAIRS 

probioticswatch@danone.com 

www.e-probioticswatch.com 

San Fransisco, USA 

http://www.nature.com/ 

natureconferences/mmi2011/index.html 

Realized with iNTUiTiO and Strat & Gérie, 2010 

29

PROBIOTICS WATCH - Probiotics In Practice

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