14.07.2014 Views

NOVEDADES EN CLSI 2008 Estándares CLSI -2008

NOVEDADES EN CLSI 2008 Estándares CLSI -2008

NOVEDADES EN CLSI 2008 Estándares CLSI -2008

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Confirmacion de KPC<br />

• Detección del Gen bla KPC<br />

• Test modificado de Hodge<br />

– Test fenotípico que puede usarse para<br />

determinar si la susceptibilidad reducida a<br />

carbapenemicos es mediada por<br />

carbapenemasa.<br />

Modified Hodge Test<br />

neg<br />

neg<br />

pos<br />

neg<br />

neg<br />

1. Swab E. coli ATCC<br />

25922 onto plate to<br />

create lawn (1:10<br />

dilution of McF 0.5).<br />

2. Place imipenem disk in<br />

center.<br />

3. Streak test isolates from<br />

edge of disk to end of<br />

plate.<br />

4. Incubate overnight.<br />

5. Look for growth of E.<br />

coli around test isolate<br />

streak - indicates<br />

carbapenem-<br />

hydrolyzing enzyme.<br />

Photo courtesy of J. Patel<br />

49<br />

Presentación J Hindler <strong>CLSI</strong> <strong>2008</strong><br />

14

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!