Radioaktiv märkning
Radioaktiv märkning
Radioaktiv märkning
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Biomedical Radiation Sciences<br />
Labeling methods<br />
Halogen labeling<br />
Indirect labelling<br />
(Bolton-Hunter reagent)<br />
+<br />
protein NH2 N<br />
O<br />
protein NH C<br />
pH 9.5-9.0<br />
CH2 CH2<br />
O<br />
O<br />
O<br />
O C<br />
CH2 CH2<br />
I*<br />
OH<br />
I*<br />
OH<br />
“+” mild labelling conditions<br />
“-” lower yield in comparison<br />
with direct labelling
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