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20 KONGRESS strong effect on the expression of EZH2 by reducing the TNF-α-mediated upregulation by 105±14% (IKK2) and 47±17% (JNK) in RASF and 86±17% and 81±4% in OASF (p
Conclusion: Our results show that VEGF aggravates fibrosis in vitro and in vivo. Potential mechanisms include direct profibrotic effects via PDGF receptors and indirect profibrotic effects by the induction of inflammation via VEG- FR1 signalling on monocytes. Thus, our data suggest VEGF as a novel link between vascular injury, inflammation and onset of fibrosis in SSc. 07 Specific histone deacetylases (HDACs) modulate fibrosis and angiogenesis in systemic sclerosis (SSc) Hossein Hemmatazad, Britta Maurer, Hanna Maciejewska Rodrigues, Renate E. Gay, Beat A. Michel, Steffen Gay, Oliver Distler and Astrid Jüngel Purpose: Recently, we have shown that Trichostatin A (TSA) does not only inhibit the activity of HDACs but also modulates significantly the transcriptional expression of two isoforms of HDACs, HDAC3 and 7. While the expression of HDAC7 is almost completely down-regulated by TSA, HDAC3 expression is up-regulated. Furthermore, we demonstrated that silencing of HDAC7 has antifibrotic effects in skin fibroblasts from patients with SSc. However, there are reports indicating that silencing of HDAC7 affects angiogenesis in human umbilical vein endothelial cells (HUVECs). Since microvascular damage is characteristic for SSc, we investigate here the role of HDAC3 in fibrosis and in angiogenesis in human uterine microvascular endothelial cells (HUMECs). Method: Over-expression of HDAC3 in SSc skin fibroblasts and HUMECs was achieved by transfecting a HDAC3-expression vector/plasmid into cells using Amaxa nucleotransfection. An empty vector was used as control. Gene expression patterns of collagen types I and III, connective tissue growth factor (CTGF) and intercellular adhesion molecule-1 (ICAM-1) were analyzed by Sybr Green Real-time PCR. To assess the expression on the protein level, antibodies against collagen types I and III, CTGF and ICAM-1 were used for Western blot. Endothelial capillary sprouting was assessed using tube formation assay. Results: After over-expression of HDAC3 in skin fibroblasts from patients with SSc, the transcription of collagen types I and III was reduced by 38 ± 16 % and 25 ± 20 % (n=6 each, p
Seite 1 und 2: www.rheuma-schweiz.ch Inhalt Editor
Seite 3 und 4: TRAUDEL SAURENMANN Kinder-Rheumatol
Seite 5 und 6: Eine dicke Wade mit seltener Ursach
Seite 7 und 8: Abb. 4: Biopsie der Skelettmuskulat
Seite 9 und 10: einer Wadenhypertrophie gekommen is
Seite 11 und 12: Je nach Lokalisation spricht man da
Seite 13 und 14: Richtlinien zur Therapie der Polymy
Seite 15 und 16: und Das erfolgreiche Original zum G
Seite 17 und 18: Ein weiterer interessanter Beitrag
Seite 19: ACR 2009 STEFFEN GAY Das ACR-Meetin
Seite 23 und 24: ACTEMRA BEWEGT. Direkt, konsequent,
Seite 25 und 26: Herzliche Gratulation Muriel Keller
Seite 27 und 28: Reviews 15. Senolt L, Vencovský J,
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