18 - World Journal of Gastroenterology

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Iannelli A et al . Subtotal colectomy for colonic inertia 2595 constipation. Aust N Z J Surg 1996; 66: 525-529 31 Dykes S, Smilgin-Humphreys S, Bass C. Chronic idiopathic constipation: a psychological enquiry. Eur J Gastroenterol Hepatol 2001; 13: 39-44 32 Hasegawa H, Watanabe M, Baba H, Nishibori H, Kitajima M. Laparoscopic restorative proctocolectomy for patients with ulcerative colitis. J Laparoendosc Adv Surg Tech A 2002; 12: 403-406 33 Hong D, Lewis M, Tabet J, Anvari M. Prospective comparison of laparoscopic versus open resection for benign colorectal disease. Surg Laparosc Endosc Percutan Tech 2002; 12: 238-242 34 Wexner SD, Reissman P, Pfeifer J, Bernstein M, Geron N. Laparoscopic colorectal surgery: analysis of 140 cases. Surg Endosc 1996; 10: 133-136 35 Ho YH, Tan M, Eu KW, Leong A, Choen FS. Laparoscopicassisted compared with open total colectomy in treating slow transit constipation. Aust N Z J Surg 1997; 67: 562-565 36 Iannelli A, Fabiani P, Mouiel J, Gugenheim J. Laparoscopic subtotal colectomy with cecorectal anastomosis for slowtransit constipation. Surg Endosc 2006; 20: 171-173 S- Editor Zhu LH L- Editor Alpini GD E- Editor Liu Y www.wjgnet.com
PO Box 2345, Beijing 100023, China World J Gastroenterol 2007 May 14; 13(18): 2596-2599 www.wjgnet.com World Journal of Gastroenterology ISSN 1007-9327 wjg@wjgnet.com © 2007 The WJG Press. All rights reserved. RAPID COMMUNICATION Effects of granulocyte-colony stimulating factor on peritoneal defense mechanisms and bacterial translocation after administration of systemic chemotherapy in rats Celal Cerci, Cagri Ergin, Erol Eroglu, Canan Agalar, Fatih Agalar, Sureyya Cerci, Mahmut Bulbul Celal Cerci, Erol Eroglu, Mahmut Bulbul, Suleyman Demirel University, School of Medicine, General Surgery Department, Isparta, Turkey Cagri Ergin, Pamukkale University, School of Medicine, Clinical Microbiology Department, Denizli, Turkey Canan Agalar, Kırıkkale University, School of Medicine, Infectious Diseases Department, Kırıkkale, Turkey Fatih Agalar, Kırıkkale University, School of Medicine, General Surgery Department, Kırıkkale, Turkey Sureyya Cerci, Suleyman Demirel University, School of Medicine, Nuclear Medicine Department, Isparta, Turkey Correspondence to: Celal Cerci, Suleyman Demirel University, School of Medicine, General Surgery Department, Modernevler 3103 sok. No.16, 32200, Isparta, Turkey. celalcerci@yahoo.com Telephone: +90-505-2933423 Fax: +90-246-2234736 Received: 2007-01-18 Accepted: 2007-03-01 Abstract AIM: To investigate the effects of granulocyte-colony stimulating factor (G-CSF) on peritoneal defense mechanisms and bacterial translocation after systemic 5-Fluorouracil (5-FU) administration. METHODS: Thirty Wistar albino rats were divided into three groups; the control, 5-FU and 5-FU + G-CSF groups. We measured bactericidal activity of the peritoneal fluid, phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid, total peritoneal cell counts and cell types of peritoneal washing fluid. Bacterial translocation was quantified by mesenteric lymph node, liver and spleen tissue cultures. RESULTS: Systemic 5-FU reduced total peritoneal cell counts, neutrophils and macrophage numbers. It also altered bactericidal activity of the peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid. 5-FU also caused significant increase in frequencies of bacterial translocation at the liver and mesenteric lymph nodes. G-CSF decreased bacterial translocation, it significantly enhanced bactericidal activity of the peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid. It also increased total peritoneal cell counts, neutrophils and macrophage numbers. CONCLUSION: Systemic 5-FU administration caused bacterial translocation, decreased the bactericidal www.wjgnet.com activity of peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid. G-CSF increased both bactericidal activity of the peritoneal fluid and phagocytic activity of polymorphonuclear leucocytes in the peritoneal fluid, and prevented the bacterial translocation. We conclude that intraperitoneal GCSF administration protects the effects of systemic 5-FU on peritoneal defense mechanisms. © 2007 The WJG Press. All rights reserved. Key words: Granulocyte-colony stimulating factor; 5-Fluorouracil; Bacterial translocation; Peritoneal defense mechanisms Cerci C, Ergin C, Eroglu E, Agalar C, Agalar F, Cerci S, Bulbul M. Effects of granulocyte-colony stimulating factor on peritoneal defense mechanisms and bacterial translocation after administration of systemic chemotherapy in rats. World J Gastroenterol 2007; 13(18): 2596-2599 http://www.wjgnet.com/1007-9327/13/2596.asp INTRODUCTION Chemotherapy is one of the most important methods in the therapy of malignant diseases. It has been widely used and it is well known that systemic chemotherapy has immunosuppressive effects [1] . 5-Fluorouracil (5-FU) has side effects, including leucopoenia, stomatitis, appetite loss, diarrhea, and mild fever like other chemotherapy agents [2,3] . It has been reported that a high dose of 5-FU often induces cytotoxicity in intestinal tissue that results in ulceration, diarrhea, and bacterial translocation [4-8] . Bacterial translocation is one of the most important causes of sepsis and multiple organ failure. Intestinal mucosa has a barrier function that prevents the colonization of the bacteria and the passage of bacteria and their toxins from the intestinal system into the systemic circulation. It has been shown that bacterial translocation is also augmented by shock, mesenteric ischemia, thermal injury, malnutrition, obstructive jaundice, and intestinal obstruction [9-14] . Granulocyte-colony stimulating factor (G-CSF) is a cytokine that is used to reverse the neutropenia associated with cytotoxic chemotherapy bone marrow and haemopoietic stem cell transplantation [15] . The role of GM-
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