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Analysis Method Reference range Requirement Comments<br />

Coproporphyrine I + III<br />

Coproporphyrine isomers<br />

Cortisol<br />

Cortisol, free<br />

Page 36<br />

men 11.0 - 23.0 µmol/l<br />

12.8 - 25.8 µmol/l<br />

< 50 µg/d<br />

0.12 - 0.37 µmol/l<br />

< 23000 µg/kg<br />

197.0 - 751.0 µg/l<br />

HPLC < 120 µg/d<br />

< 0.1 µg/dl<br />

< 6 µg/g<br />

HPLC Coproporphyrin I 17 - 31 %<br />

Coproporphyrin III 69 - 83%<br />

Coproporphyrin I 60 - 75%<br />

Coproporphyrin III 25 - 40 %<br />

RIA 8.00 - 10.00 h 68 - 263 µg/l<br />

16.00 - 18.00 h 38 - 144 µg/l<br />

RIA adults 8 - 77 µg/d<br />

4 month - 10 years 2 - 30 µg/d<br />

EDTA-blood 2 ml<br />

urine 5 ml<br />

liquor 1 ml<br />

faeces 2 g<br />

liver tissue 0,3 g<br />

mother milk 10 ml<br />

urine 10 ml<br />

P: Sample of 24-hour collection,<br />

protect from daylight by wrapping<br />

into aluminium foil, keep refrigerated<br />

plasma 1 ml<br />

P: Hemolysis must be avoided, send<br />

plasma or serum refrigerated<br />

faeces ca. 2 g<br />

P: Keep and send refrigerated,<br />

protect from daylight (wrap with foil)<br />

urine 10 ml<br />

P: Sample of 24-hour collection,<br />

protect from daylight by wrapping<br />

into aluminium foil, no additives<br />

faeces ca. 2 g<br />

P: Protect from daylight (wrap with<br />

foil)<br />

serum 1 ml<br />

urine 10 ml

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