ELISA Development Guide - OriGene

ev022912
Step 29 Add 100 l Blocking Buffer to each well
Step 30
One well at a time, mix wells by pipetting up and down 5 times and then remove
the solution containing suspended beads from the well.
Step 31 Transfer the solution ( 100 l) to a new labeled tube.
Step 32 Store beads @ 4ºC in the dark.
(Optional). Count the beads to determine the bead concentration using a
hemocytometer or a particle counting instrument such as a Cellometer.
Step 33
Alternatively, assume the beads are at a concentration of 10,000 beads per l and
adjust the concentration after initial testing.
OriGene Technologies, Inc. 9620 Medical Center Drive, Suite 200, Rockville, MD 20850
www.origene.com techsupport@origene.com 1-301-340-3188 (P) 1-301-340-9254 (F) Page 22