ELISA Development Guide - OriGene
ELISA Development Guide - OriGene
ELISA Development Guide - OriGene
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ev022912<br />
Coupling Buffer (Luminex)<br />
(50mM MES, pH 5.0)<br />
To prepare 1000 mL, add the following to 900 ml of deionized water:<br />
MES (2[N-Morpholino] ethanesulfonic acid) 9.76 g<br />
Mix thoroughly, then add 5N NaOH to bring the pH to 5.0.<br />
Bring the volume to 1000 ml with deionized water.<br />
Verify that pH is 5.0-5.1.<br />
Filter sterilize through a 0.2 m filter unit.<br />
Store at 4ºC.<br />
Check pH monthly or before use.<br />
Blocking Buffer (Luminex)<br />
To prepare 1000 mL, add the following to 900 ml of deionized water:<br />
10X Wash Buffer<br />
(Part No. AR100010 – 100 ml)<br />
(Part No. AR100011 – 1000 ml)<br />
100 mL<br />
BSA 1 g<br />
Sodium Azide 0.5 g<br />
Mix thoroughly until the solution is homogeneous. Filter sterilize through a 0.2 m filter unit.<br />
Store at 4ºC.<br />
<strong>OriGene</strong> Technologies, Inc. 9620 Medical Center Drive, Suite 200, Rockville, MD 20850<br />
www.origene.com techsupport@origene.com 1-301-340-3188 (P) 1-301-340-9254 (F) Page 40
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