ELISA Development Guide - OriGene

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Basic ELISA Protocol
Materials
Description Recommended Source
Antibody coated microplate See previous section.
Assay Buffer
For dilution of detection antibody,
secondary antibodies, and
streptavidin conjugates.
Assay Diluent
OriGene has four different Assay Buffers, each containing a
different optimized mixture of buffer, salts, detergent and
proteins. We recommend starting with Assay Buffer D.
Different buffers may perform better than others
depending upon the species origin of the capture and
detection antibodies.
Assay Buffer A - contains mouse and goat IgG Part No.: AR100012
Assay Buffer B- contains BSA Part No.: AR100013
Assay Buffer C- contains BSA and rabbit IgG Part No.: AR100014
Assay Buffer D- contains BSA, mouse and goat IgG Part No.: AR100015
Serum-based diluent for dilution of
standards and samples.
OriGene, Part No.: AR100016
Contains heat-activated animal serum, detergent and a
preservative.
Biotinylated Detection Antibody OriGene biotinylated detection antibodies are supplied at
0.5 mg/ml.
ELISA Wash Buffer (10X) OriGene Part No.: AR100010 (100 ml), AR100011 (1L)
Streptavidin-HRP Conjugate OriGene Part No.: AR100017
TMB Substrate, soluble, 1-part OriGene Part No.: AR100018 (100 ml), AR100019 (1L)
Stop Solution OriGene Part No.: AR100020 (100 ml), AR100021 (1L)
Absorbent paper towels WYPALL X60 towels Kimberly Clark
OriGene Technologies, Inc. 9620 Medical Center Drive, Suite 200, Rockville, MD 20850
www.origene.com techsupport@origene.com 1-301-340-3188 (P) 1-301-340-9254 (F) Page 6