Chapter 15 | Multi-Staining Immunohistochemistry - Dako
Chapter 15 | Multi-Staining Immunohistochemistry - Dako
Chapter 15 | Multi-Staining Immunohistochemistry - Dako
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<strong>Multi</strong>-<strong>Staining</strong> <strong>Immunohistochemistry</strong><br />
Conclusion<br />
<strong>Multi</strong>ple-target staining will one day be a routine procedure just as<br />
single-target staining is today. Use of the technique will extend, since<br />
it offers reduced turnaround-time and information not obtainable<br />
from single-target staining. availability of reagents that give a wider<br />
range of possibilities when it comes to choice of technique, such as<br />
e.g. labeled primary antibodies and antibodies raised in different<br />
host species, will likely increase. In addition, some suppliers now<br />
offer complete kits with clinically-relevant antibody cocktails and<br />
visualization systems optimized to give the correct, balanced stain,<br />
thus significantly reducing the workload for the user.<br />
Software for automated image acquisition and analysis will play<br />
a key role in this evolution since the limit to how many colors the<br />
human eye is capable of distinguishing is limited. analysis algorithms<br />
will never entirely replace a skilled pathologist, but algorithms will<br />
gradually improve as the amount of information loaded into underlying<br />
databases increase. eventually, algorithms will become sufficiently<br />
“experienced” to be able in many cases to suggest a diagnosis, and<br />
only the final decision will be left for the pathologist.<br />
108 | IHC StaInIng MetHodS, FIFtH edItIon<br />
References<br />
1. Molinié V et al. Modern Pathology 2004; 17:1180-90.<br />
2. Van der Loos CM, J Histochem Cytochem 2008; 56:313-28.<br />
3. Van der Loos CM. Immunoenzyme <strong>Multi</strong>ple <strong>Staining</strong> Methods, BIoS<br />
Scientific Publishers Ltd. 1999.<br />
4. Chaubert P et al. Modern Pathology 1997; 10:585-91.<br />
5. Van der Loos CM and göbel H J Histochem Cytochem 2000; 48:1431-7.<br />
6. Merryn V.e. Macville analytical Cellular Pathology 2001; 22: 133-42.<br />
7. Mason d Y et al. J Pathol 2000; 191:452-61.<br />
8. dickinson Me et al. Bio techniques 2001; 31: 1272-8.<br />
9. Landon a. et al. the Journal of Pathology, 2003, 200: 577-88(12).<br />
10. Förster th., Fluoreszenz organischer verbindungen, Vandenhoeck &<br />
Ruprecht, 1951.<br />
11. Wu X et al. nat Biotechnol 2003; 21:41-6.<br />
12. the small, small world of quantum dots. CaP today.december 2008.
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