SERION ELISA classic Measles Virus IgG / IgM - virion\serion
SERION ELISA classic Measles Virus IgG / IgM - virion\serion
SERION ELISA classic Measles Virus IgG / IgM - virion\serion
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YOUR<br />
GLOBAL<br />
PARTNER<br />
IN<br />
DIAGNOSTICS<br />
<strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong><br />
<strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong> / <strong>IgM</strong><br />
serion<br />
The <strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong> and <strong>IgM</strong> tests are qualitative and quantitative<br />
immunoassays for the detection of human antibodies in serum or plasma directed against<br />
<strong>Measles</strong> <strong>Virus</strong>. The <strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgM</strong> test is recommended for the<br />
determination of acute infections whereas the <strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong> test<br />
is used for determination of immune status and for demonstration of intrathecally synthesized<br />
antibodies for CSF diagnostics.<br />
Electron micrograph of a <strong>Measles</strong> <strong>Virus</strong><br />
(Source: Centers for Disease Control and Prevention,<br />
United States Department of Health and<br />
Human Services)<br />
multianalyt TM<br />
Pathogen<br />
<strong>Measles</strong> <strong>Virus</strong>es occur worldwide and belong to the family<br />
of paramyxoviridae. According to estimations by WHO one<br />
million individuals die as a consequence of <strong>Measles</strong> <strong>Virus</strong> infections<br />
every year. Due to the highly infectious nature of the<br />
virus, measles is usually aquired in the childhood. The pathogen<br />
is transmitted by close contact with infected individuals<br />
primarily by droplet infection.<br />
Disease<br />
After an incubation period of 10 to 12 days Koplik´s spots<br />
typically appear on oral mucosa in 60 to 70 % of cases. In the<br />
course of the prodromal period flu-like symptoms may escalate<br />
until the typical measles exanthema arises with sharply<br />
increasing fever. The exanthema spreads from face over the<br />
trunk to the extremities. The clinical symptoms peak rapidly<br />
with a correspondingly rapid recovery. Complications are frequent<br />
consisting mainly of pneumonia and encephalitis.<br />
Infection results in a life-long immunity. A combination of<br />
attenuated live vaccines for mumps, measles and rubella<br />
(MMR) has been available for several years.
Diagnosis<br />
Demonstration of <strong>IgM</strong> antibodies is commonly used as evidence<br />
of an acute infection and produces positive test results<br />
early on with the onset of symptoms. The demonstration of<br />
<strong>IgG</strong> antibodies serves primarily for immune status and vaccination<br />
control as well as for confirmation of <strong>Measles</strong> <strong>Virus</strong><br />
infections. The detection of <strong>IgG</strong> antibodies in CSF samples is<br />
particularly recommended in cases of suspected encephalitis.<br />
<strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong>/<strong>IgM</strong><br />
For validation of the <strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong><br />
69 serum samples with different antibody reactivities were<br />
analyzed in an external study. 50 serum samples from healthy<br />
blood donors were additionally tested in house. A commercially<br />
available <strong>ELISA</strong> was used as a reference test. For<br />
validation of the <strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgM</strong><br />
86 serum samples of patients with suspected acute measles<br />
infection were analyzed in an external study. An <strong>ELISA</strong> of a<br />
leading European manufacturer was used as reference test.<br />
Highlights of <strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong>/<strong>IgM</strong><br />
● Use of an inactivated <strong>Measles</strong> <strong>Virus</strong> preparation (Strain Edmonston)<br />
● Sensitive <strong>IgM</strong> detection for the diagnosis of acute infections<br />
● Demonstration of <strong>IgG</strong> antibody activity for confirmation of <strong>Measles</strong> <strong>Virus</strong> infection,<br />
for immune status determination and vaccination control with results expressed in mIU/ml<br />
referenced to the international standard of the World Health Organization (WHO)<br />
● Excellent sensitivity and specificity values<br />
● High precision and linearity within the measurement range<br />
● Detection of intrathecally synthesized <strong>IgG</strong> antibodies for CSF diagnostics<br />
Order Information<br />
<strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong> Order Nr.: ESR 102 G<br />
<strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgM</strong> Order Nr.: ESR 102 M<br />
<strong>SERION</strong> <strong>ELISA</strong> control <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong> Order Nr.: C 102 G<br />
<strong>SERION</strong> <strong>ELISA</strong> control <strong>Measles</strong> <strong>Virus</strong> <strong>IgM</strong> Order Nr.: C 102 M<br />
Please visit our website www.virion-serion.com for more information on our <strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> products.<br />
Institut Virion\Serion GmbH<br />
Serion Immundiagnostica GmbH<br />
Friedrich-Bergius-Ring 19<br />
97076 Würzburg, Germany<br />
Sensitivity and Specificity<br />
<strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> Sensitivity Specificity<br />
Measels <strong>Virus</strong> <strong>IgG</strong> > 99 % 93.3 %<br />
Measels <strong>Virus</strong> <strong>IgM</strong> 95.4 % > 99 %<br />
Precision<br />
<strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgG</strong><br />
Sample Mean<br />
value<br />
OD<br />
Intraassay<br />
CV (%)<br />
(n=20)<br />
Tel.: +49 (0)931 3045-222<br />
Fax: +49 (0)931 3045-100<br />
dialog@virion-serion.de<br />
www.virion-serion.com<br />
Mean<br />
value<br />
OD<br />
Interassay<br />
CV (%)<br />
(n=10)<br />
negative 0.296 4.4 0.352 9.7<br />
positive 1.142 4.3 1.420 9.1<br />
strong positive 2.087 2.3 2.432 7.8<br />
<strong>SERION</strong> <strong>ELISA</strong> <strong>classic</strong> <strong>Measles</strong> <strong>Virus</strong> <strong>IgM</strong><br />
Sample Mean<br />
value<br />
OD<br />
Intraassay<br />
CV (%)<br />
(n=20)<br />
Mean<br />
value<br />
OD<br />
Interassay<br />
CV (%)<br />
(n=10)<br />
positive 0.631 2.2 0.600 9.4<br />
strong positive 2.591 4.3 3.065 7.5<br />
strong positive 2.810 3.1 3.520 8.1<br />
Institut Virion\Serion GmbH, V3.12/10