Recent advances in ovulation synchronization and superovulation in ...

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Abstracts. II International Symposium on Animal Biology of Reproduction, Nov. 19-22, 2008, São Paulo, SP, Brazil. Messenger RNA quantification of bone morphogenetic protein-6 (BMP-6) in goat ovarian follicles J.R.V. Silva1, I.R. Brito1, C.C.F. Leitão1, I.M.A. Frota2, M.V.A. Saraiva2, A.P. Almeida2, J.R. Figueiredo2 1 Federal University of Ceara, Biotechnology Nucleus of Sobral, Sobral, CE, Brazil; 2 Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, CE, Brazil. Introduction The identification of the biological functions of autocrine/paracrine factors that are produced in the ovary is very important to understand the mechanisms controlling folliculogenesis. Some of these molecules are synthesized and secreted by the oocyte and/or granulosa cells and control follicle growth and differentiation. Bone morphogenetic protein-6 (BMP-6) has been identified in rat ovarian follicles and some evidences indicate that it controls granulosa cells activity (Shimasaki et al., 2004). However, in goats, relatively little information is available on the local factors that regulate folliculogenesis. The aim of the present study was to perform the quantification of mRNA for BMP-6 in goat ovarian follicles. Material and Methods Ovaries (n = 30) of goats were collected in local slaughterhouses. Ten ovaries were dedicated to isolate primordial, primary and secondary follicles, using a mechanical tissue chopper. The remaining ovaries were used to collect COCs and mural granulosa / theca cells after dissection of small antral follicles ( 0.05). In rats, BMP-6 mRNA expression is rapidly decreased at the time the dominant follicle is selected and may be linked to the mechanisms of follicle selection. In vivo studies have demonstrated that BMP-6 inhibits progesterone production induced by FSH (Otsuka et al., 2001). When the expression of BMP-6 was compared between COCs and granulosa / theca cells of small or large antral follicles, no significant difference was observed (P > 0.05). In rats, BMP-6 is also expressed in both oocyte and granulosa cells (Shimasaki et al., 2004). In goats, the protein for BMP-6 has been localized in oocyte and granulosa cells of both preantral and antral follicles (Silva et al., 2007). It is concluded that, an increase in the production of BMP-6 is observed during transition of primordial to primary follicles in goats and that both COCs and granulose/theca cells from antral follicles produce similar levels of BMP-6 mRNA. References Otsuka F, Moore RK, Shimasaki S. 2001. J Biol Chem, 276:32889-32895; Shimasaki S, Kelly Moore R, Otsuka F, Erickson GF. 2004. Endocr Rev, 25:72-101; Silva JRV, Brito IR, Leitão CCF, Silva AWB, Passos MJ, Fernandes LA, Vasconcelos GL, J.R. Figueiredo. 2007. Acta Sci Vet, 35(supl):1044. Financial support: CNPq, FUNCAP and IFS E:mail: jrvsilva@ufc.br Anim. Reprod., v.6, n.1, p.207, Jan./Mar. 2009 207
208 Abstracts. II International Symposium on Animal Biology of Reproduction, Nov. 19-22, 2008, São Paulo, SP, Brazil. Repeatability of follicle diameters and hormones in mares E.L. Gastal 1 , M.O. Gastal 2 , J.C. Jacob 2 , M.A. Beg 1 , O.J. Ginther 1,2 1 Department of Pathobiological Sciences, University of Wisconsin, Madison, WI 53706, USA; 2 Eutheria Foundation, Cross Plains, WI 53528, USA. Introduction Measurable repeatability within an animal has been shown in association with number of follicles and superovulation rates in cattle and sheep. In humans, the number of antral follicles had a measurable degree of repeatability in individuals between menstrual cycles. The few repeatability studies performed in these species have been concentrated mainly in follicle numbers. Mares are good comparative research models for follicle studies because of many similarities with women in the dynamics of the ovulatory wave. In mares, repeatability has not been studied previously for any reproductive characteristic. The purpose of the present studies was to characterize the within-mare repeatability between two consecutive intervals for follicle, FSH, LH, and estradiol dynamics in spontaneous (experiment 1) and ablation-induced (experiment 2) follicle waves. Materials and Methods Transrectal ultrasonographic examination of the ovaries and blood sampling for hormone assays were performed daily. Numbers of follicles were grouped into diameter classes in each experiment. In experiment 1 (n=23 mares), the experimental period extended from 4 days before the first ovulation to 4 days after the third ovulation (ovulation = Day 0). Diameters of follicles ≥15 mm were measured and diameters of all other follicles (2 to
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