Recent advances in ovulation synchronization and superovulation in ...

Abstracts. II International Symposium on Animal Biology of Reproduction, Nov. 19-22, 2008, São Paulo, SP, Brazil.
Oct-4 and vimentin expression in Agouti paca embryos
A.L.R. Franciolli 1 , C.E. Ambrósio 1 , D.S. Martins 1 , C.V. Wenceslau 1 , F.R. Alves 1 , R.E.G. Rici 1 ,
M.R.F. Machado 3 , A.C. Morini 1 , A.F. Carvalho 2 , M.A. Miglino 1
1 Dept. of Surgery, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, Brazil; 2 Dept. of Morphology, UNIfeob,
São João da Boa Vista, São Paulo, Brazil; 3 Paulista State University, Jaboticabal, São Paulo, Brazil.
Introduction
Research involving stem cells showed that the protein OCT-4 is the most used as a marker of pluripotential mobile
(1) and first described in embryonic cells of mice (2). The vimentin is a marker of mesenchyme of mesoderm
embryonic cells and also used in embryos to colocalized as fibroblasts intermediary filament (3). The objective was
to identify pluripotential of embryonic cells of Agouti paca through immunomarked with OCT-4 and vimentin.
Materials and Methods
They were used 2 embryos of Agouti paca with 1.8 cm of Crow-rump (CR) and another with 1.2 cm of CR, set in
paraformaldeyde 4%, dehydrated and embedded in paraffin. Sequential slides of 5µm were carried out, followed by
the processing for the immunohistochemistry.
Results and Discussion
The Oct-4 as a marker of pluripotential showed up in the heart positively to the embryo of 1.2 cm in CR in the
region of myocardium, as well for the kidney in both embryos in the tubules region and, liver, as in a important body
haematopoiesis in the hepatocytes cords. These findings corroborate to the results of literature, which cites that Oct-
4 expressed in cattle is located in blastomere, in the internal cell mass of the blastocysts, in epiblast and embryonic
stem cells (4), as well as lines of cells from human tissues of adult liver, heart and bone marrow (5). Also note that
the same was not expressive in the lung, intestine and somites for the two embryos. The vimentin as a marker of
mesenchyme cells and the embryonic mesoderm made up positive in both embryos, as in the lungs and bronchi in
the region of capillaries that nourish the region of epithelial cells. In the heart, this marker was positive in microvessels,
mesenchyme and heart. In the intestine mesenchyme around the intestinal tubules and blood capillaries
proved to be positive for vimentin, but also in somites, which were found around the cartilage and mesenchyme. In
the liver, regions found positive for vimentin were mesenchyme cells and the endothelium of sinusoids, while in the
kidney, positive in mesenchyme and was seen in the region around the kidney tubules and the capillaries. As in our
results, the term positive for vimentin mesenchyme of embryos in pigs (6) and human (7) is expressed in cells of
epiblast and hypoblast, which brought the mesoderm and endoderm, respectively.
References
(1) Vejsted M, Offenberg H, Maddox-Hyttel P. 2006. Reprod Fertil Dev, 18:1-2; (2) Okamoto K, et al. 1990. Cell,
60:461-472; (3) Franke WW, et al. 1978. Proc Nat Acad Sci USA, 75:5034-5038; (4) Kurosaka S, Eckardt S,
McLaughlin JK. 2004. Biol Reprod, 71:1578-1582; (5) Beltrami AP, et al. 2007. J Am Soc Hematol, 110:3438-
3446; (6) Prelle K, Holtz W, Osborn M. 2001. Anat Histol Embryol, 30:339-344; (7) Moore KL, Persaud TVN.
2004. Embriologia clínica. 7. ed. São Paulo: Elsevier Ltda. 609 pp. [in Portuguese].
Financial support: FAPESP
E-mail: andrefranciolli@yahoo.com.br
Anim. Reprod., v.6, n.1, p.243, Jan./Mar. 2009 243