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Abstracts. II International Symposium on Animal Biology of Reproduction, Nov. 19-22, 2008, São Paulo, SP, Brazil. Evaluation of luteolytic effects of Sodic cloprostenol (Sincrocio®) in Mangalarga (Equus cabalus) mares treated in different days of estrous cycle E.D.F. Souza 1 , S.S. Carreiro 1 , J.R.G. Maio 1 , P.D.C. Fleury 2 , T.F.R. Ometto 2 , A.A. Teixeira 3 , J.N.S. Sales 3 , G.A. Crepaldi 3 , P.S. Baruselli 3 1 Ouro Fino Saúde Animal, Ribeirão Preto-SP. 2 Central Fleury de Reprodução Eqüina, São José Rio Pardo-SP; 3 Animal Reproduction Department, FMVZ/USP, Brazil. Introduction The induction of ovulation in mares is a practice to optimize the reproductive management and improve the use of stallions. Thus, the objective of the present study was to evaluate the luteolytic effect of Sodic cloprostenol (Sincrocio®) administered in different days of estrous cycle to induce the heat in cyclic mares. Material and Methods In the present study, it was used 53 cyclic mares showing good body condition. The animals that presented follicles >35mm were treated with 2500IU of hCG (Chorulon®, Intervet, Netherlands) to induce ovulation. Only the mares that ovulated in the same day (Day 0; n = 29) were used at this study. The animals were allocated in one of three experimental groups [Day 3 (n=9), Day 7 (n=10) and Day 12 (n=10)]. Animals from each group received 250 µg of Cloprostenol (PGF) i.m. in different days of the estrous cycle (Day 3, Day 7 and Day 12. Blood samples were collected immediately before and after the PGF administration (0, 12, 24, 36 and 48 h). The samples were cooled, centrifuged (3000xg/5minutes) and then frozen to measure plasma progesterone concentrations by radioimmunoassay (Coat-A-Count Progesterone® (Diagnostic Products Corporation, Los Angeles, EUA). Results and Discussion The plasma progesterone concentration was significantly different before the administration of PGF on Day 3 compared to Day 7 and Day 12. The P4 concentration did not differ between treatment performed on Day 7 and Day 12 in any moment of the blood collection and decreased until 48 h after the administration. The P4 concentration was similar between all groups only in 12 and 24 h after the treatment. The P4 concentration of Group Day 3 decreased lesser (36 and 48 h) than Day 7 and Day 12 groups. According to these results, we can conclude that 250 µg of Sodic cloprostenol (Sincrocio®) was efficient to induce luteolysis when the treatment was performed on Day 7 and Day 12 after ovulation. Progesterone (ng/mL) 10 Figure 1. Plasma progesterone concentration immediately before and after (0, 12, 24, 36 and 48 h) the PGF administration 3, 7 and 12 days after ovulation (* Day 3≠Day 7 e Day 12, P
238 Abstracts. II International Symposium on Animal Biology of Reproduction, Nov. 19-22, 2008, São Paulo, SP, Brazil. Cellular changes in the uterine tube epithelium of the mare J. Aguilar *1 , M.B. Rodriguez 1 , M. Reyley 1 , V. Rivarola 2 , B. Rumie Vittar 2 1 Producción Equina, Facultad de Agronomía y Veterinaria, 2 Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Ruta 36 km 601, 5800 Río Cuarto, Córdoba, Argentina. Introduction The uterine tube is a key organ of the reproductive tract where major biologic events occur. It is documented that the epithelium of the uterine tube experiments changes throughout the reproductive phases in many species. In the cow, marked cyclic changes were described on the surface of the epithelium in the fimbriae and ampulla mainly, and fewer changes were found in the isthmus and uterotubal junction (1). Similar changes have also been described in the pig (2), the bitch (3) among other species. Briefly the main changes occur at the height of the cells, the proportion of ciliated and non ciliated cells, and the presence of secretory granules. One particular and interesting finding consists in protrusion of cytoplasmic extension towards the oviductal lumen; sometimes large cell fragments including nuclei and whole cells are also observed being extruded from the epithelium. Particularly in the mare, information available on cellular changes of the oviductal epithelium is very limited. Therefore, the objectives of this study were to investigate morphological changes in the oviductal epithelium of the mare at different reproductive stages. Materials and Methods Sixteen non-pregnant mares, aged 2-14 years, were selected at a local slaughterhouse plant and assigned to be sacrificed at different times. Reproductive status was determined by rectal palpation and ultrasonography. Four mares were selected at the following stages: a) anovulatory phase, with follicles less than 15 mm, b) estrus, with follicles of 35 mm or larger and uterine oedema, c) diestrus, at day 7-8 post ovulation, and d) postovulatory mares, at day 1-2 post ovulation. Reproductive tracts were collected immediately after the mare´s death and placed in ice. The oviduct was freed of connective tissue and samples from the ampulla, ampullary-isthmic junction (a-i-j) and isthmus were taken for histopathology (Hematoxylin/Eosin) and stained also with Hoescht 33258 for identification of nuclei by fluorescent microscopy. Results and Discussion Marked histological changes in the epithelium of the uterine tube were observed associated with the reproductive stages. Ciliated and nonciliated cells were present in the three regions at all stages examined, with ciliated cells constituting the major cell type. Cell height varied among the stages being shorter at the anovulatory phase. At diestrus, the epithelial cells showed a very consistent location of the nuclei at the base of the cells. In contrasts, in estrus and much more at postovulation, the nuclei were found at different locations within cells, many toward the apical surface or even protruding into the lumen. Some times whole cells were found like being “pushed or eliminated” to the lumen, this was mostly observed in the ampulla and the a-i-j. The morphological appearance of these cells was not apoptotic but would indicate some kind of renewal process. This study confirms for first time that in the mare, epithelial extrusion of large cell fragments including nuclei and whole cells also occurs. The mechanism of this process of cell elimination seems to vary among species. Non-apoptotic cell loss of non-ciliated cells occurs in large (pig, sheep, goat, cattle) and small animals (dog; 4). However, apoptosis was commonly observed in many areas of the cat oviduct epithelium. References (1) Abe H, Oikawa T. 1993. Anat Rec, 235:399-410; (2) Abe H, Hoshi H. 2008. Reprod Dom Anim, 43:66-73; (3) Steinhauer N, Boos A, Günzel-Apel A-R. 2004. Reprod Dom Animals, 39:110–119; (4) Steffl M, Schweiger M, Sugiyama T, Amselgruber WM. 2008. Ann Anat, 190:46-52. *Email: jaguilar@ayv.unrc.edu.ar Anim. Reprod., v.6, n.1, p.238, Jan./Mar. 2009
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