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M"y, L974 - MSpace at the University of Manitoba

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1" 5 CIILTURAI, PRACTICES<br />

1.51 Inocul<strong>at</strong>ion<br />

Lupins, like o<strong>the</strong>r legumes, store large amounts <strong>of</strong> proÈein in <strong>the</strong>ir<br />

veget<strong>at</strong>ive parts and seeds" ProËein syn<strong>the</strong>sis is dependent upon organíc<br />

nítrogen, which is obtained from <strong>the</strong> symbíosis bett¡een <strong>the</strong> root system<br />

and <strong>the</strong> bacteritur Rhizobiwn Lupini. Thís particular bacËerium is classi-<br />

fied into <strong>the</strong> lupín inocul<strong>at</strong>ion group and will not function with o<strong>the</strong>r<br />

legurne species (Erdman, 1953).<br />

Norris (1956) and Graharn (1964) found th<strong>at</strong> <strong>the</strong> 1upín nodule bacterium<br />

differs in many respects from <strong>the</strong> Rhizobit¡n classific<strong>at</strong>ion" They are re-<br />

1<strong>at</strong>ívely slow growing and can survive 1ow soíl ferËility and pH, <strong>the</strong>refore<br />

Graham suggests th<strong>at</strong> this inoculaËion group be transferred Ëo Ëhe genus<br />

Phytomyæa"<br />

Ne1 (1960) found th<strong>at</strong> ínocul<strong>at</strong>ion is not always necessary" Yield<br />

decreased when uninocul<strong>at</strong>ed seed was groÍrn on land th<strong>at</strong> had not carried<br />

lupins for seven years, however no yield decreases resulted when uninocu-<br />

l<strong>at</strong>ed seed rrras groltn on land Ëh<strong>at</strong> was cropped with lupins Ëwo years pre-<br />

víous. McKee and Ritchey (L947) suggested th<strong>at</strong> inocul<strong>at</strong>ion should be<br />

caxried out every year, even on land prevíously cropped with lupins"<br />

Schinidt (L967) found ËhaË some indigenous strains <strong>of</strong> lupin inoculum<br />

are parasític" Ilowever, if an active straín was placed on <strong>the</strong> seed prior<br />

to plantingo it prevenÈed infection by <strong>the</strong> inactive parasitic strain"<br />

Differences in strain effíciency exíst. Kretovich et a1. (L972) found<br />

a correl<strong>at</strong>ion between intensity <strong>of</strong> respir<strong>at</strong>ion, cyÈochrom. P450 "td<br />

effeetiveness <strong>of</strong> t1ne Rhizobiun strain" Ineffective strains had onLy 257"<br />

<strong>of</strong> <strong>the</strong> respir<strong>at</strong>ion <strong>of</strong> effective strains and conËained no cytochrom. P450"<br />

13

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