Intensive Survey of the Guadalupe River Segment 1806

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Selection of a specific test site must be made in the field by the investigator with the depth, velocity, and benthic substrate taken into consideration. At the test site the dissolved oxygen meter, and strip-chart recorder are calibrated, the respirometer is dry tested by opening and closing switches and testing batteries; a stream velocity measurement is taken (for paddle calibration), and a water sample is collected just above the stream bottom near the sampling site. Portions of this water sample are poured into separate BOD bottles, one of which is opaque. The opaque bottle is placed on the respirometer and left for the remainder of the test. The initial dissolved oxygen value in the other bottle is measured when the test begins, while the dissolved oxygen in the opaque bottle is measured at the end of the benthic uDtake test. The difference in the two dissolved oxygen values represents the oxygen demand of the water column. The respirometer can be lowered from a boat or bridge, or can be placed by hand in shallow streams. Care is taken to insure that the sediment at the test location is not disturbed and that a good seal between the base of the instrument and bottom of the stream is made. After the respirometer has been placed in the stream, the dissolved oxygen is recorded. In shallow, clear streams the instrument is covered to prevent photosynthesis from occurring within the chamber. The test chamber is then closed and the paddle frequency adjusted. Recordings of dissolved oxygen are made until oxygen is depleted within the chamber or 6 hours has elapsed. Paddle Frequency f = 36 v where: f = Paddle frequency in revolutions per minute Velocity to be simulated in m/s (measureed with current meter) Benthic Oxygen Uptake BTD01-D02 = 196 (D0i-D02) - B0Dt where: BTDO1-DO2 = Oxygen uptake rate in g/m2/d corresponding to the sample temperature, T At DO] = Initial DO reading in mg/1 DO2 = Final DO reading in mg/1 A-6
Laboratory Analyses Unit of Parameter Measure Arsenic (As) mg/kg Mercury (Hg) mg/kg All other metals mg/kg Volatile Solids mg/kg Chemical (COD) Oxygen [ )emand mg/kg Kjeldahl (Kjel-N) Nitrogeri mg/kg Total Phosphorus (T-P) mg/kg as P Pesticides yg/kg Oil and Grease mg/kg BACTERIOLOGICAL At = Time interval between DO, and D0~ T = Temperature of sample in °C BODt = Measured difference in DO between the two BOD bottles Method Silver diethylidithcocarbonate method(3). Potassium permanganate digestion followed by atomic absorption(3,4), Atomic absorption(3,4). Ignition in a muffle furnace(3). Dichromate reflux method(3). Micro-Kjeldahl digestion and automated colorimetric method(3). Ammonium molybdate(3). Gas chromatographic method(4,5). Soxhlet extraction method(3). Bacteriological samples are collected in sterilized bottles to which 0.5 ml of sodium thiosulfate is added to dechlorinate the sample. Following collection, the samples are stored on ice until delivery to a laboratory or until cultures are set up by survey personnel (within 6 hours of collection). Bacteriological analyses are conducted by survey personnel or a suitable laboratory in the survey area. Parameter Total Coliform Fecal Coliform Fecal Streptococci Unit of Measure Number/100 ml Number/100 ml Number/100 ml A-7 Method Membrane filter method(1) Membrane filter method(l) Membrane filter method(1)
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INTENSIVE SURVEY OF GUADALUPE RIVER
Page 3 and 4: ABSTRACT An intensive survey of Gua
Page 5 and 6: TABLE OF CONTENTS CONTINUED Page Be
Page 7 and 8: METHODS Field and laboratory proced
Page 9 and 10: wheat and oats are grown in the reg
Page 11 and 12: 40, and 400 mg/L (annual averages).
Page 13 and 14: indicating the highest need for str
Page 15 and 16: consideration. An abundance of orth
Page 17 and 18: increase in the importance of pollu
Page 19 and 20: CONCLUSIONS The historical data and
Page 22 and 23: TABLE 1 Guadalupe River Sampling Lo
Page 24 and 25: to Station Location IH 10 in Comfor
Page 26 and 27: to CO Station/SMN Number Center Poi
Page 28 and 29: SO CJI Station Location/ SMN Number
Page 30 and 31: to *3 5.65 200 4.52 -- -- 1 60 RR 3
Page 32 and 33: Figure 4 Location of Hydraulic Meas
Page 34 and 35: Figure 5 Location of Cross Sectiona
Page 36 and 37: TABLE 8 Guadalupe River Field Measu
Page 38 and 39: TABLE 8 CONTINUED Map Code Water Di
Page 40 and 41: 1800.9010 11/01/83 COMP 0.3 20.0 4.
Page 42 and 43: CO CD Map Code Station Number of In
Page 44 and 45: Map Code Station 0STRAC0DA Taxon St
Page 46 and 47: 4* CO Map Code Station Taxon EPHEME
Page 48 and 49: TABLE 12 Percentage Composition of
Page 50 and 51: APPENDIX A >.fl&^fc- .. - -.^K&ST
Page 52 and 53: Laboratory Analyses Parameter Five
Page 56 and 57: BENTHIC MACROINVERTEBRATES 2 Benthi
Page 58 and 59: Samples are stored in quart cubitai
Page 60 and 61: where DOj = initial dissolved oxyge
Page 62 and 63: Samples are transferred to the labo
Page 64 and 65: REFERENCES CITED 1. American Public
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Intensive Survey of the Guadalupe River Segment 1806

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