Intensive Survey of the Guadalupe River Segment 1806

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BENTHIC MACROINVERTEBRATES 2 Benthic macroinvertebrates are collected with a Surber sampler (0.09 m ) in riffles and an Ekman dredge (0.02 m2) in pools. Samples are preserved in 5 percent formalin, stained with Rose Bengal, and sorted, identified, and enumerated in the laboratory. Diversity (d) is calculated according to Wilhm's(6) equation: s d = - E (n-j/n) log2 (nj/n) 1 where n is the total number of individuals in the sample, nj is the number of individuals per taxon, and s is the number of taxa in the sample. Redundancy (r) is calculated accordinq to the eouations derived by Young et al.(7) (1) d max = log2 s (2) d min - - s'] n " l0
PERIPHYTON Periphyton are collected from streams and reservoirs from natural sub strates or from artificial substrates placed in the water. Standard size, frosted microscope slides are commonly used as artificial sub strates and are held in place a few centimeters beneath the water surface at the sampling sites in floating periphytometers. Following a 25 to 30 day incubation period the accrued materials are analyzed for chlorophyll a_, pheophytin a^, and for identification and enumeration of the attached organisms. In the field, following retrieval of the periphytometer, two slides are placed in a brown glass container containing 100 ml of 90 percent aqueous acetone. The material from these two slides is used for pigment measure ments. Two slides are placed in another brown glass container containing 100 ml of 5 percent buffered formalin. The material from these two slides is used for biomass measurements. The remaining slides are also placed in buffered formalin and utilized for identification and enumeration of organ isms according to procedures discussed for the phytoplankton. The brown glass jars containing the material for laboratory analyses (pigment and biomass measurements) are placed in a deep freeze and kept frozen prior to analysis. The autotrophic index is calculated accordina to the equation given by Weber and McFarland(9). a 4. 4. u- i a Biomass (g/m2) Autotrophic Index = ^—- Chlorophyll a^ (g/m2) Periphyton samples may also be collected from natural substrates by scraping areas from each type of substrate available at each sampling location. Scrapings are made from a range of depths from subsurface to the stream bottom, from bank to bank, and at points spanning the range in stream velocity. The scrapings from each sampling location are composited into a container, preserved with Lugols solution and returned to the laboratory for identification and enumeration following procedures discussed in the phytoplankton section. Diversity, redundancy, and equitability statistics are calculated as described previously. PLANKTON Phytoplankton Stream phytoplankton are collected immediately beneath the water surface with a Van Dorn sampler or by immersing a sampling container. Phyto plankton samples are collected with a Van Dorn water sampler at depths evenly spaced throughout the water column of reservoirs. A-9
Page 1 and 2:
INTENSIVE SURVEY OF GUADALUPE RIVER
Page 3 and 4:
ABSTRACT An intensive survey of Gua
Page 5 and 6: TABLE OF CONTENTS CONTINUED Page Be
Page 7 and 8: METHODS Field and laboratory proced
Page 9 and 10: wheat and oats are grown in the reg
Page 11 and 12: 40, and 400 mg/L (annual averages).
Page 13 and 14: indicating the highest need for str
Page 15 and 16: consideration. An abundance of orth
Page 17 and 18: increase in the importance of pollu
Page 19 and 20: CONCLUSIONS The historical data and
Page 22 and 23: TABLE 1 Guadalupe River Sampling Lo
Page 24 and 25: to Station Location IH 10 in Comfor
Page 26 and 27: to CO Station/SMN Number Center Poi
Page 28 and 29: SO CJI Station Location/ SMN Number
Page 30 and 31: to *3 5.65 200 4.52 -- -- 1 60 RR 3
Page 32 and 33: Figure 4 Location of Hydraulic Meas
Page 34 and 35: Figure 5 Location of Cross Sectiona
Page 36 and 37: TABLE 8 Guadalupe River Field Measu
Page 38 and 39: TABLE 8 CONTINUED Map Code Water Di
Page 40 and 41: 1800.9010 11/01/83 COMP 0.3 20.0 4.
Page 42 and 43: CO CD Map Code Station Number of In
Page 44 and 45: Map Code Station 0STRAC0DA Taxon St
Page 46 and 47: 4* CO Map Code Station Taxon EPHEME
Page 48 and 49: TABLE 12 Percentage Composition of
Page 50 and 51: APPENDIX A >.fl&^fc- .. - -.^K&ST
Page 52 and 53: Laboratory Analyses Parameter Five
Page 54 and 55: Selection of a specific test site m
Page 58 and 59: Samples are stored in quart cubitai
Page 60 and 61: where DOj = initial dissolved oxyge
Page 62 and 63: Samples are transferred to the labo
Page 64 and 65: REFERENCES CITED 1. American Public
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Intensive Survey of the Guadalupe River Segment 1806

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