Standard Operating Procedures for Bull Management (1088.47 KB)

Post thaw evaluation
a. After 24 hours period in LN the frozen semen straws are re evaluated for post
thaw motility
b. One straw should be thawed at a time
c. Each straw is removed from the container by using a forceps
d. Jerk the straw once to remove any LN in the factory seal end and put into the
thawing unit or water bath maintained at 37 degree C and thaw for a period of 30
seconds.
e. Evaluate the post thaw motility (PTM) by repeating the procedure for 2 to 3
straws per bull / batch
f. Forward motility under the phase contrast microscope (200X) with a warm stage /
bio therm is observed
g. Only samples that show a PTM of 50% and above should be preserved
h. Those below 50% PTM should be discarded.
Quarantine of semen doses
a. The frozen semen accepted for long term storage should be held in the laboratory
for a period of one month as a quarantine mechanism
b. During this period if there is no out break or incidence of disease the semen is
passed on for dispatch / sale / bulk storage.
Laboratory hygiene
a. The laboratory personnel should be technically competent and observe high
standards of personal hygiene to preclude the introduction of pathogenic
organisms during semen evaluation, processing and storage.
b. The work tables, all surfaces of Cold handling unit and other horizontal surfaces
are mopped clean with 70% alcohol at the end of each work day.
c. Formalin humidification is done twice a week on Wednesday and Saturday by use
of 12 ml formaldehyde solution in 100 ml of water per cubic meter of lab area
including the AV preparation room (on Wednesday – weak solution for
humidification).
d. The bacterial load may be monitored regularly to see the efficacy of sterilization
by exposing agar plates in all rooms of the lab
e. All laboratory footwear and protective clothing should be cleaned and washed
daily prior to use the next day.