Standard Operating Procedures for Bull Management (1088.47 KB)

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The air space or air bubble at the end of the straws is created by another plastic comb on to the prongs of which the straw in the clamp is fixed. The action expels some semen and thus the air space is created. The comb has a plastic backing and both are attached to the disposable container containing the semen and the semen expelled from the straws runs back into the container. For time saving and to eliminate contamination, the container and comb are discarded after each bull. The air bubble or space at the open end of the straw is essential so that the column of semen in the straw can extend during the freezing process. 4-Sealing- The powder for sealing is spared evenly and compressed firmly over plate to a thickness of 5 mm A glass plate is very necessary to obtain good plugging because lumpy and clotted powder sticks to the surface of the glass and does not penetrate into the powder plug. The open end of the straws held in clamps is pressed with a firm regular motion into the clean powder until it forms a plug in the straws of 4 to 5 mm. 5-equilibration in Straws Immediately after plugging the straws are released from the clamps and immersed for two hours in water container at +4degree centigrade to allow for equilibration of the sperm with the constituents of diluents and glycerol. At the same time, this operation allows the plug to become firmer and the excess powder on the end of the straws falls to the bottom of the water container.
6-cleaning and drying After equilibration, the straws are held in bundles in the hand and are tapped in the bottom of the bath to ensure good plugging and to remove particles of powder. The better the cleaning and drying, the greater advantage will be found in ease of counting and storing. Ice will form on damp straws and this will cause difficulty in counting and reduce storage space and freezability. The out put for two persons using this method will be about 4500 straws per hour. Annexure-2 Health status and disease prevention, testing of the Quarantine bulls ( Isolation ) Number : Breed : Date of birth: Dam : Date of introduction into the Center : Name of the owner from whom purchased: Body weight at birth (in kgs) : Sire :
Page 1 and 2: TABLE OF CONTENTS Sl.No. C h a p t
Page 3 and 4: Standard Operating Procedures for B
Page 5 and 6: Except for very tender and succulen
Page 7 and 8: In places where the individual bull
Page 9 and 10: Preparation of bulls for collection
Page 11 and 12: Hygiene and Precautions required by
Page 13 and 14: g. Hold the AV before and after eac
Page 15 and 16: . Standard Operating Procedures (SO
Page 17 and 18: d. No staff from the semen collecti
Page 19 and 20: ONE STEP DILUTION Match extender te
Page 21 and 22: d. A ply wood/acrylic sheet is plac
Page 23 and 24: Chapter - 4 Standard Operating Proc
Page 25 and 26: Definition Description 0- None No m
Page 27 and 28: B. Wet technique From the thawed fr
Page 29 and 30: -B- 0.25 ml of frozen thawed semen
Page 31 and 32: Stock solution B 1. Nacl -9.01 gm A
Page 33 and 34: Volume) inactivated and sterilized
Page 35 and 36: 2. Water bath capable of being main
Page 37 and 38: SELECTING A SOLUTION FOR DILUTION:
Page 39 and 40: Area of 80 small squares = 1/400X80
Page 41: -Prepare semen smear and allow it t
Page 45 and 46: Sl. No Date of completion of Quaran
Page 47 and 48: Annexure-5 Q.C. test conducted duri

Standard Operating Procedures for Bull Management (1088.47 KB)

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