Standard Operating Procedures for Bull Management (1088.47 KB)

Standard Operating Procedures
for
Frozen semen Quality Control parameters
1.Post – thaw Viability
The post-thaw Viability of spermatozoa is dependent primarily upon recovery from
freezing without membrane damage. The following parameters are assessed.
1. Percent of Progressively motile cells.
2. The rate of progression.
3. Percent intact acrosome.
1. Motility Examination
Objective:
Examination of motility in post-thaw frozen semen sample.
Discussion:
Sperm motility must be considered one of the most important parameters in evaluating the
fertility potential of semen specimen. The type of movement will also influence fertilizing
capacity. Thus sperm swimming in tight circles cannot readily pass through the utero-tubal
junction, and only straight swimmers succeed in fertilizing ova. Vigorous beating of the
sperm tail is necessary for penetration of the sperm head through the corona radiate to
fertilize the ovum.
Assessment of sperm movement
Quantitative assessment
For the purpose of semen analysis, sperm motility may be recorded as the “Percentage
motility” i.e. the percentage of the total sperm that are showing movement. The estimation
of percentage motility should be performed immediately after thawing. Each estimation of
motility should be performed in duplicate and the mean result recorded.
Qualitative assessment
The quality of sperm movement is usually graded according to the type of movement made
by the largest proportion of the total sperm. The grading used can be numerical or
descriptive, if numerals are used; sperm movement is graded from 0-5. The scoring system
recommended is as follows.