Standard Operating Procedures for Bull Management (1088.47 KB)

25 big chamberx16 small chambers =400 small squares
=1sq mm
No of sperms in area 80 small squares =N
Area of 80 small squares = 1/400x 80=1/5 sq mm
Depth =1/10 mm
Volume in 80 small square =1/50 C mm(1/10x1/5)
No of sperms in 1/50cu.mm area =N
No of sperms in cu mm area =Nx50
No of sperms in 1ml of semen =50Nx1000
Dilution Factor =50Nx100x1000
Total No of sperms/ml =Nx106
To calculate exact number in usable volume.
N = 5N X 106 x 0.48 (French medium) N = 5N X 106 x 0.23 (French Mini)
9. DNA STAINING OF SPERMATOZOA
Objectives:
Feulgen’s staining technique for examination of DNA
Discussion
The Feulgen staining technique is specific for DNA and allows closer scrutiny of nuclear
defects such as vacuolation and abnormality in shape .It also improve reliability in evaluating
the knobbed acrosome defect in that the nucleus is always flattened or indented at the apex.
The technique helps to clearly examine sperm nucleus as it results in magenta –deep purple
staining of only the DNA, while the acrosome and tail are not stained.
TECHNIQUE-