Methods for the determination of 3-MCPD-fatty acid esters
Methods for the determination of 3-MCPD-fatty acid esters
Methods for the determination of 3-MCPD-fatty acid esters
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Strategy <strong>for</strong> <strong>the</strong> Development <strong>of</strong> BfR methods<br />
General Objectives<br />
to determine exclusively 3-<strong>MCPD</strong> FAE<br />
to <strong>of</strong>fer a sufficient robustness <strong>for</strong> adoption in routine labs<br />
Direct Method versus Indirect Method? Method<br />
Direct methods like LC-MS/MS <strong>of</strong> <strong>the</strong> <strong>esters</strong><br />
- guarant high specificity<br />
- require <strong>the</strong> availability <strong>of</strong> <strong>the</strong> complete „set <strong>of</strong> standard materials“<br />
- require very low LOD/LOQ <strong>for</strong> each single 3-<strong>MCPD</strong> FAE<br />
- <strong>of</strong>fer a limited robustness<br />
Indirect methods like GC-MS <strong>of</strong> <strong>the</strong> free 3-<strong>MCPD</strong><br />
- <strong>of</strong>fer an increased robustness<br />
- involve hydrolysis and derivatization<br />
- might induce chemical modifications <strong>of</strong> <strong>the</strong> analyte<br />
Final Decision <strong>for</strong> <strong>the</strong> development <strong>of</strong> indirect methods<br />
However, with <strong>the</strong> additional obligation that several methods should be<br />
tested applying different types <strong>of</strong> hydrolysis.<br />
hydrolysis<br />
Angelika Preiß-Weigert 18 May 2010 3rd Meeting AOCS Expert Panel on Process Contaminants page 5
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