Page 1 .76 .. 1993 ANNUAL MEETING ABSTRACTS Session A ...

.76 

Session A Monday Morning 

Computer Integrated Manufacturing (CIM) I 

ComlH"t'f"1J.1~led M.nuf.,;turl"l Onrrie .... Rkt Remon. Cqillinc .• P.O. 

8oJI9JOO. MinrapoIi .. MiMelOlfo jS440. ond D. S.w.l. Arizona SIaIe Univ=ily. 

OM ~udI Cmlo:r. Tnnpe. AZ 8l2a3. 

Cornpurer IeChnoIoI)'.ppticd IICn)SI tile KlUOlspemum of m.nufacturing an 

yield • stroIIl compelil;~e poIi!ion. The ~ &iVftlIO br...t ...., of c:ompula'< in aU 

flCCll of ........ r.:turinl inc.udin. pnIC!ftIina is oompw ... imqpaIcd 11WIII00000ng 

(ClM). In !he opmi"l pl'UmlJilion of 1M ClM T«hnOl Scs&ioo, aU aspects of !he 

topic ....ilI be acncnl1y uploral inc:1udinl infomwion ond control sysloms design. 

implmimc.tion and operation from ",W 1I\II1m.l proo;:umnent. Ihtoogh processing, 

ond II> shipmmt ollhe finiJhal poOducIL The purpose of !he overview is to give a 

dear definition of the IIIbjecl and 10 IOeI lbe SIa,e for lbe subsequent detailed poumwionf 

on the !ipplicalion of OM. 

Computer Inlflralfd M.nllflldurlnl- Riel; Btnson, Cargill Inc.• P.O. Box 9300, 

MinneapoIi .. Minnesota 'S440. 

OptnIionaIliUCttU lIartJ with.)'JWlI des.ign. This truism is especlcaion. lltcsc is..... 

and _pproprio ... conliderll(ioo n'hnrdw,,", lItId IOflw.n:options will be eovened in 

the presentation. Economic and other benefit. of balch COIltrol system, will be discuSllol'd.Thc 

prornlncnce of balch jII'OCeJIconlml in lhe wool. romp",er inlepltd 

manuf""'lurina ,y"em will beeJplaintd. 

ComPl't..- Conlrol Appllfd 10 OIMd ~Inl- N. Wine. eonsulWII.6631 Par_ 

IOItI Coun. Fl. W.yne.IN 468]$.Joitn E. Hrilmon. Heil ...... Consulting Group. 

613S Moorf'lC'ld A~e .. Colondo SpOn,.. Colondo 809194802. 

Applielllioo ofcompuler eomrol in oi]Ked pmccning is pining prumineno: in 

...... y potU of the world. New pi...... bcina iMUollcd in Europe .... Taiwan ...,. for 

the _ 1*1. compW"- ronnollcd. In the Uniltd SIIICS. IlIcU1 one oiloccd pmccs- 

IOf hal uli]ized com ........ CIlIIIrol for ,.,~en1yean. Accordingly • .we-of'_the·an 

computer """,aion in oilKed proecuin& ...ill be mrie..-..! in apprupriaIc deWI_ 

The funcrion 01 pmanrnmable Joaic COOIIrollcn and lhe ability 0I1lIpCI'Yi:oDry com- 

~ U) _ roIl«lC'd doIIIo ror _ic:iplli", ct.o..... ~ and alanning the 

~ oI........ionI; ClDIIUOlII"I prot:aI ...-.riabIa; and driwing vidtognphia 

w[ll be aplcnd. The ...., ~ IIMonced oofIwano U) perform dala =onriIillion, proalii 

optimiDiion and prot:aIlimulIlion ... ill be diKuued ""th ""I'P"fting exam-- 

.... 

U"lHIltaIklHd. 11MRrsI a..lhor Ibled 1ii.15o IlIotipHk ..... 

INFORM. Vol. 4. no. 4 (AprIl 1993) 

1993 ANNUAL MEETING ABSTRACTS 

.. 

ComPl't..- Conlral Appilfd 10 EclI~ 011 f"ronSlll-l~ Castel ......... _I. 

lam. P.O. Bo.\ ]4030. Palm DeIen. CA 9WS-40lO. Norm Smallwood, The Con: 

Team. P.O. 801 ]32]. Iiunmond. Lwiliana1(140.1, MdJL. Kintkhuo. PSII'rocaI 

S)'SIl'mS Inc:.. 1790 Kirby Pkwy .. SuilO:300. Memphis. TN 38138. 

Swtinl in 1981 with lhe A.E. SlIley plant in Des Moines. most ~ the new 

edible oil jII'OCesai... planiS tomIrutltd in !he Uniltd Swes have included com· 

put..-control. Funhermon!. in IIlOII uilfll\l United SI.les plants. proanmm.blc: 

lock cOIItmllen llave b«n in ... lltd 10 eontrotapecif'" pnxes!oltf when: jllSlilitd. 

lbe COIIlJ>Ut..-eonlfollY'tem .n:hiIKlun: .. on .... i(h detailed bardwan: and JOf'I_ 

ware options .,.uenily available ror appllcalion in edible oil ~n8 will be 

elllllined.1lIc benefil •• jwtifit.tion and lilllificllIlCe ~ tomput.r- ....... cOllIfol 

in tdibl. oil prvccUinl will be up~ in lhe COIlint of computer ;nttar-Itd 

manUfIOCIUrin•. 

Session B Monday Morning 

Polymers/Builders lor Laundry and 

Cleaning Products 

Sponsored by tne AOCS Surfactants and Detergents 

Division 

" 

Buildfl' Systtmsln Dd..--ml formulatiom.. Amo Cahn. Amo CIhn CmsulIinl 

So-vicei.lnc~ nEM! Allison A_. Pud Ri ...... New York 10963. 

lbe cfraty~lIlOIIourf~ Iii advtnpenld pmvido 1Idd;(ionral fko~ibilily in formuhuins 

"tonctnlra,«!" liquid produa •• s tompan:d 10 OIher tbelat;n. Igen15 commonly 

used in det.'II" ...... However. to CI\iO)' lbe ben.fi" ofullns ~eoli(es. one must fiM 

sol"e!he sedlmenuulon pmblemlllllOCi.led wilh ,lie IncorporaliOll ofwaLer_insolu_ 

ble solid~ in liquids. Rl>tololl)' oflhe del"'II"OlllOlu1ion and ltOlilc panicle aiu pIll' 

major roleti in IklermlninSIlll: IICdlmcnl~llon Nleofuolltel. The vis.:oclaslic rhcolosids. 

SUUClURd liquid dclergcnI pm:IlIeIl an: ClIpIIbIc of .... pending ..ndiuohtd par_ 

licles while mnainil\l potulIbIe with adequate riIeolo&ieal performance. One way of 

RrU

ha~ hydrupl'lilic pms whlcll_ well hydnlcd in the concmlJ'alcd oah ooIution (EP 

34699~J. Usil1l ~ poIymrn.. HCp dIan~in;~ in~ionc.n "" 

oIuinai. Tho. 1cchnoloIY 1150provides meh II"IoC:ft f1Qlbiliry in the choia: or the 

diffcmll romponmb 10 be inccIrpon.Icd in liquid dctefgem fonnulalions (WO 

91m844.9L,(III281). 

C.klum "-"chanle Ptrfurn.an« of7.eo1Ue N.AlNI1COj Mixlu~ 

R.P. Omkcwiod 02761-89) have hmI u.cd 10 roulintly _ ..... _y 

0( STPP. However. \"len when dli. meIhud iicompletely .... omaICd. only one ...... 

pic an be yud per hour. Carel\d ftI'Iipmen! ~ and hi'~ytr2ined 

openIOn IIR. ~irecllO ~ Jood ~I~ Man: ==dy l p nuclar mas' 

OVIk resonano:e{NMR) hal been UICd to ~ ee _yofSTPP ..... pIes and to.. 

been found to ~ • ..-produc:ible and accul"llle meIhod. Howevcr ...... am and "f'"Cial 

requifI:mmII 0( NMR inluumenwion JftCllIde ilS use in. pnxess cumroIlaboraIo- 

1)'. A new meIhud Ulln" modified Speo;tra- Tech ~ ATR IWI1.pIinJ 

1CCeSSOf)'0II a Bomem MB·I20 FouricrTransfonn-lnf....-ed (FT-IR) spectnJtneItf 

.... been dev,,1oped for ~11ho: lOWly0( 5TPP IWI1.pIes. Nine pcrccnI 

5OIU1ionsof STPP in deionized w .. er an: ~ and added 10a 4S· ZnSe ATR 

samplin, boll. The ATR lp«UUm i. obcr.incd by lCanIIing tho. 9'1> STPP solulion 

bet.. ecn 1400 and U.s .....vmum~ .. for IS min at 2 ..... """""'be! re$OIution. Five 

spectral 11m lOR' then IUCd to calculate IhI: lUIy 0( Iho: STPP ..... pIc usin. Galactk: 

Indusuies SpectrI Cak'" and PLSplusllO soft",,", ....ilh. calibration model that was 

developed ""inl ..... I'T-IR ~I", oflhiny diffemJI 5TPP samples. wtoose assay 

..... .,.......;...wy de1trmlncd by lip NMR. Compu-ison show ...... FT-IR method 10 

be .. accul"llle ....... lip NMR melhod and 

.. 

..... r.pid and limplccnough th.ot il can 

ee u!!fld for pn:oo:::t.' CIIOIIrot of the .... y of STPf' during iu produaion. 

477 

SKS-6: The Nt ... Mulll·Btownt Buildtr. Ib,.._~.,.. Ria:k. GlInt""" Scbimmel and 

F""l·J~fOany. 11oechs! AQ. 62JO Frantfun .... Main 80. Germany. 

Silie.teII hi"" been widely used in dtlc'1em formulations. The !IOlub!e 

amorphoulliOdium 5ilic .. es (.....Ier ..... ) serve .. alkalinily sou= and anticomot'lion 

agent: in!iOluble oodium .luminosiliC"alc uoliles Ilre WIfd for walet tut.nIncscJ 5f([ueI- 

Imlion. SIo ....ly I(.tuble "yen:tIry on Turbid tor SolId Llpld..Qonllllnlnl 

~-oodSampld rlN" Rlpld Quality Assessmenl. Willian. L Poner. Edward D. Black 

and Doullas Granl. U.S. Army NlIlick RD&:E Cent.,... Kansas 511ft!. NlIlick. MusachuiiCl!s 

0 1760. 

Rmliomttric nlCIOOds for imemal calibnuion of f""'I-f_ fl~ 

r.peclropholomc:ll)' permil rapid. ",liable asses:omenl of ""venol rcaclion. aff«lins 

food quality. including lipid o~ida1ion, cblornphyU degnodalion and o:atOIeroe alleralion.ln 

lIddilion. lhe ~tial diK"rimination (aciliwed by....race fluoromc:uy of 

lOlid roods makeo thoe rcaclMIns potenlially _ful as intrinsic n:cordtrJ 0( Imtpe1llure 

bi.wry ",ilh;n food puticlc$. ptmIided that Iho: kinnia of!ho: rcaclloru; are 

blown. Mctl.otb of thi. typc an: dc:5cribcd for lhe clllorop/lyll-phI:ophylin tl"lU"lSition 

in srcm vegetables and fruilS ...... aystaltine 10 lipid-di"""~cd camceroedoanJ

478 

CJ 

Tho IkhlVlor ofl'bollphollplds In Slyrqel Silte-f..uluskln Chl'Ollllllotnphy: Do 

Tht1' Col~ "Ith Po(yIltft'kTri&I~! R.G.Ackman md EJ. MKpbenon. 

T..:hnio::aI Un'Ymi.), 01 Nov. Scotia. 8011 1000. Halifu, Nova Scotia. BlJ 2X4 

""""- 

OYer IWO dcadcI ... "'''icy cotumn chroa1IIopaphy 01'1 S!)'m'Ie diviny~ 

copolymer beads pvc aood ~ of .,.-Iipids. cqenriaUy ~ootipido...m 

1ri&I)'t'Uides. on • ..,. 0102 J. ~ popaIIriIy ol_,,!*atX --.up:ii_y ~ 

~rinlhc~~~(HPl..C)macII: ........ 

10 t..-e ben! I'C$II'Dod 10 rn- sa&.d Dill ond uxd (and obascd) fryinc oils. In _ 

of ~ wei... _ r ....:11: phosphoIipidI; shuuId c:oinc:iok willi ai&!)'Qerida. 

We M....IIOIC'd consisIenl e ollRllaial fnIm fHh IIIIl$I;:Ie -'-i ollhe 

lri&/yurilk poM in HPl.C wiIh twO ultraslyTa&lel XK) A 001 ........ in ocrio:o ..t 1ttI1Iby- 

..., funn.1he eIuaM.. We (mel 111M nann! fish IIMde phoiphoIipids JUdI &5 IhMe ot 

cod _If:, """ indoaI dille ohead olai&lJ=idcs. whik 1).,"""~'oIipidJ.• upen- 

.... duro: mud! 1Ia:r. Thio; II:I'YCS as._ 01calIion Ipinst lICafJlinIaU peIIb duIinc 

bdCft monomo:rit; Iri&l~ &5 ~ and frimrft; ui&l~ in Il)TI&d em.. 

IIIIIIIpfIIly of Dill and r.s. This ......... cspa:iaIJy apply ItI &yina op:nOo:Q """'"' 

IIVIIeriaI udIanp would be ~ 

Otttnnln.11on of UpId Hydropmu;lcIes by Hlp.l'trfonnana: Uqald 

CbromalOVllphy "..qlll"", .. Uh Multl-Chan ..... Ult... viokl Dn~or. Y~hiro 

TOI.m IIId ScHuko HAIa. FlIClihy ofEngincerinS. Seikei University,l-. Kichijoji· 

kitarnachi l, Mu.suhino-ihl, Tokyo 18(1,Japan. 

Hish-performance liquid chronwography (HPLC) was developed forthe deter· 

minalion 0( uch lipid hydropcro~ide cbs!; in c:atain nall1fll1lipidl; mixiUlC$, ItJ_ 

HPLC ~\I$eqllippcd willi. muiti..:hanneluluaviolet (UV)deIcctorMd. 

.I;&btl)' ",yened-p/we .iliea CI column was used for !he ~i OAidi«d r... _ oill were oimillt \0 _ 01 o.Ibdienal.1rIII,Ior alkcnab. 

indialina 1hII the: oani.ity _ ....wy due! ... thcie aIddI)'Ik:I.. Wbik: """ IIIduct 

deftlopmn!l frQO> .... ~ver _ brain homogen;ucswu op!;maI .. pH l-4 ond 

~ by t-butyl h)'dn>po'fw.ido ond f.rno: ion, it .......... camplefdy ~ 

by EDTA. iI>dkalinglhal the ractiv~y wu due! to boIh .11

ell 

Diff~ In 1M Oxidative Slabll;l), 0( n-J and n-6 Polyunsaturated Fitly Adds 

In Aq_ Solution. ~ MiyaWta. Elichi Nan!. and TOfUOta. Hokkaido Urnvasiry. 

l-I-I. Mina/lxho. IilIkodm 041. Japan. 

Oxidali", Slabili'yo(n-l and 11-6p- 

SlnIIC roruem by gas chromalogrJpIly dtn>Ocnl of Applied OlC1IIisuy. Fa.:uIIY of 

Engineering. 0$akI Univer.tiIY. SUiID.Osaka 565. Japan. 

Enzymatic catal)l$is in organic solvems has been allracting m""h recenl.lIcn_ 

lioo IlS a rocw and promising biolechnology. In panicular. helemgeneoUlllysttml. 

where en7.ymes ~ suspended in organic solV-I}-hydrwly pmdUCI$. CIS:) w.u found 10 be. bcnc:r .ut>- 

511111" for epo~yg""ation among tl!.eso: ullSlllunted f.l1)' a.:Kk. 

Mult~nlrk Stlldy Dl'CI.-Ll.... II.I~C

480 

comparin. pWma MId m:I blood noll (RBC'llipKI ~ ~ m- poupI 

n:o;civin. _mal milk (MM). roonuLa (F... a 31-32)« IS:] n-3 mrichnl formula 

CEf. n ... 30). Tho: enriched formllia ... JUppIemenIcd willi • low erucic Icid rape_ 

seed oil (~lfIIII;'Y)~ by. 18:2 rHirfIB;J n-3 ...-rig!R taliool"6{11IId 

111:2 n-6 .. 4.94" or rouJ cnctD'). MClhoduIos:Y and !derma: .;dues aluinod w;m 

MM feedi,. _Ihc: 371l1 ...'eek 1ft- aiven in ... annued COOUlll1lUcalion.. Tho: ~II •• 

day 15 (DI~)"", HpoIt'd 10$ wt'IoofflitY Kids in plHrna lriglya:ri 

Nol:.oij'nuo0Ild Kll:(uhiko Sumki. UQIl COfll'OIl'IiQll. 100 Tajima. Odaw:mo·5hi. Kana· 

I"WI 2S6. Jlpan. 

Nt ... prodlIt:tion n'II'1hods for pro5U&Landins ha~c beto1 Mwied ""illl! I ....,;"" 

red .oll" Groci"' ........ ,I"II('O,W. G. ,...rrll('(l,JG. _ or the popular now m:um.Js for 

Ipr. ro,""im no pn>SlaAbndiM in M1u~. Howeva". unsa!unlled fmy Iridl; 

to'IIaintd in G rTJI("OJ;p ....en: found 10 M oonvtmd imo prosragIandins byenzy· 

nwk ractionJ hid! Wft"C induced by ph)'Jical Rimullllion< 10 the .olp suc:h as~· 

lina .... ha:u-th.owin&- n.. yield 0( PfOIOlIIlandim bued QIlthe dried .olga ...... 

.bcM 0.2'10. The «Jfllp05ition or mllilin. proWI&Iandins ..... ~Iucd 10 m. of_· 

"",led fmy .::ids canuinod in the .olp. ArKtIidonie .. id which is" "",in umatu ... • 

ed fony.::id in G.• -nnonua was cIIidlyconwned imo pn>SlaAlandin E2. On the 

0Ihtr hind.. .. 'httJ unsaturated fl"Y.::ids ~ irMmiloruolly odded 10 the Cfllymllic 

rnaions. ~ ... JlRlUlllandins Wft"C oIiuined. for e.umpIe. the Iddilion of 

dihomo-,\,"Iinoknic and~.::id pve ~.Iandin EI ond E3'~· 

lively. Inveu.ipUon ohhe synd>elic poch .....y using 4C·]aMIed IlnICbidoniclcid 

made ~ clear IIw prostaglandin E2""" formed vio IS·hydmpl:mxy·prouagLandin 

~,Thi.""lh .... y ..... diffm:nt from the: bioJynlhetic: ""th .....y ofprouaglandi ... in 

mamllllis. 

07 

Multlantrit: Study of a·Unokll1lte Comp~mentatlon In Pn:malure NftHlat~ I: 

MHIwt\oIogy and Rdl't'\'tttt V.lu~ althf, J7th W~d: (Hu ..... n Milk). S. Mlzeue 

lAd B. Descumps. F... af5c'icncft 3)40S Talencc Ccdox. INSERM U.S8. 60 rue de 

N,vII«llts.l409O Montpc:Uicr. France. N. ComM lAd B. Ernrr:uangles. Univenit~ 

BortkIUX I. F. Babin and A. Cru:co de Paulcl. Biochimie A. P. Sarda.. N&!n.lltaIo&ie. 

O. Bou&l~and A. Nouveiot. CHU c.....,and F. Mmely. OnidoI-Crtiom. P:lris. 

,~. 

Complm1o5phBle 

(ATl") level. in ldogen l\ajr follicles inaeased =narbbIy by the appIicoUon of 

PIX) in tIM:uaminlllion wilb nobbjiS. II WII ~ in the cultured lllir papilla (dis 

offOl vibri_ .... t ATP pnxluclion and DNA 5)'11lhesil ....en: mhont:td by the: addi. 

tion of pc:rMadecanoK: acid (PDA) into the medium without gluoooc.lt WII found in 

the miloch!>C-4.22·m ...... t.ldienr:. 

n.. ooIt.1* pret.5U~ for the mi~td 

monoI.yer of OPPC willt su:roids hiving I short side chain i.higher lhan tIw of 

OPPC with ",emills hiving I Iongsidc ~hain. At!imiluoollopse JnSSW'l'S. the tim· 

itin • ....,. of DPPC ...ith foIefidswhicb have only • f~w double bonds i.. malle. tban 

Wt ....ith Ileroids havinll more double bonds. The interxtion belwl'Cn hy 

Monolayer Properties and I... TrmrsportaIion ~ or Cf'DWn [Iller 

COIIIpoulKls I)IlTtr!ftllII Crown Size It tIM OIl/Wit ... Intman.. Kunio 

FIIfIISI_ Univenl1y of Tsuku~ of OiIttnisrry. 1·1. Tmnod&i·l. Tiuku· 

t. 30:1. HidDo ,.. ............. ElectmIechniall..ll>onlory. and TSlIfIdlilto )(u ......n" .... 

Gunma URi""",ily. Japan. 

10 ev-J....., .... 1oIIbility of c:n-n _ compcamdil ",ilb I:nndocd hydrucarbon 

dw ... diffcrin. in cmwt1 li:r.c; "' the oiVwalu (O/W) ;mn100:e, 1I>Of1OIa)'Cf propcnico 

of the compound were IfIIdiod ...... g YIriout .sail$, A liable lI>Of1OIayerof cacb crown 

«Jfllpound formod IIIthe inlerfll« under melII «JfllpIeJ.;n, conditions. Ho_. In I 

.y"'em i""lw;n •• compound,.;th small crown ";te, ion IoC'lectivityand abililY of 

«JfIIplu formllion dcttued. po6Sibly due 10 the high beal otbydration ofamaJl.

Ue iom. This abo was observed in the e1ecnotinnic behavior er ce OJW emul,"'" 

uabilized by the .arne crown ether COfl'Ipll\jndJ. 

100transport of c;rown ether compounds wU uudied wi!h diff~1 size 

compound-sail combinarions.lon ~lecti¥iIY by crown ~ in tbe ion Irmt

482 

[RZ)'malic Mudinullon of.he }'aUyAdd Conlenl 04' Phospholipids. P~lrkk 

Aldc:.-cn:ulz, Ingemar Svensson and Bl (PI). pbosphatMJylglY""fOI 

(PO) and phosp/IalidylliCri"" (PS) by en:zymc-

DopartmmI of AgrlcuJIUl1II CbemiSlf)'. The Uni~enity olTokyo. Bunlr;yo.-ru. Tokyo 

Ill,Japan. Roser N. fWdty, The Sc:roIled.., *WId ICIId 10 impnIYUIICDI 01 

aop f;!'II'l.ry by manipubling seal proICini by ""*'cuLw ..,...nl ~ We 

""~C been wom"l on gmes mcodin,!kon&lyclnin, the 7$ ICed tu>rage protein of 

.oybull. mainly using molecular biooIosil;al appI'INICha.1I was shoo>lI 111Mu~ 

.oon of lkan&lycinin gmes in ~ I~ p11R1J ....,h as l.x.cco. peru. 

"Ia and MubidopJis was rnmmI.led in • $ffii·.p«if\c maMIL'f. UsinllllU\SgmiCt ha..., bccotne more 

ouphi.ricalcd. (unher advances in uOlkrst~ndina lhe Iill\lC1u~·funcrion proponies of 

rhe m~joI' pc""ur proteins. .......,hin and cl)IUIIW;hin h~vc ruuhod. This includes 

j",proY~ cLariilcalion of the polypefllide subunit .Irucru~ of rhese .lI>r:age globu· 

Lin~.rheir inhetalt properties ... molc:cullr ronformlltOn!i. de ... lIlfalion. aggn:gation. 

pH II(IIubilily. &lpc. 8y ulllizinal ntlionaIlIC1ttnina pmpam. 

,,"'( t.,yC ioobIed and devdoped I>C'" ,..nins or.lpc·like minoorganiSfM that JIm'" 

rapidly and ptuduct L;t1]!C1l/OOun" of long chlin w3 (IIny acids (EPA. OPA and 

DHA I. Thi. palMled prono:>1osY10 am ....the 

Hili II hip deMilics in. ronuoI environmcnl. The msululllI ...·hoIc-a:1L biornoso 

tw I Lon, chain 103 COOICIIIof ov 10'l. of dry """ight. 8y ul;lizin. differenl illrains. 

I v;oriel~ ofroJ (Illy Kid profiles (""i

o;osI e«(CIive!), inCo'pDnued Inm animal fecofdOle ...en: =overed in TO and PL fraction. ~p

1M .-",wed incn:&oc of ehoIesInoi to phIetal'ymJ faUy AricU oa MHrlbl'1lM Conlenl of\'fl"1 Long Chain 

Fatty Arids (C=2-Ilo.w) In Rtiln.lland ~plQl1:ofNormai Rats. 

Miyounl Sull. Amon; A. W"ltrd,;di and M. Thomas Clandinin. Nulrilion & 

Metabolism R~ Group. Univenil)' of Alberta, 318 Hoo>e EnInomics Bldg., 

Edmonton. Albma. ean.da TOO 2M8. 

The etfttl of dietary 11)3r.ny acids on membrane (OI1I(RI of very long ch.in 

r.ny Kid, (VLCFA orc O!; 24) composition Will dc.e....i...d in IxMh (ncinl retina 

and rod ou.cr segmc:nlS (ROS). Wean' ina fillS were fed nu,riclonall, adequate 

dicl$ contain;ns (20'I>(..../W) fal of rvle rany acid com- 

",,"ilion of refina MId phoIcn«p1Of cells. 

G" 

Pho5phoIipjcl1UnIO\'~ In lhe In.tsllnal M~ oflM Pa'''nl5 _i.b Inflamma- 

.Clr180 ....d 0Isn0sa Mio:ko OW ..... Kuuko Nlbnishi. T... k.o {)ohi. E1w.10:0 

ai, TaUdti Sal1O. H~ Morita and s,obu.ro K.wai. Oivislon ofainic:al 

Yaw- 

Biochemi!iU}. Oinical R_atdI IlUIilllte II1d Depanmml of SIItJffY, National Medical 

Cmu:-r. 1-21-] Toyanoa. Shinjuku,h, Tokyo 162, J• .,.,.. 

"The preliminary rlfMling on rdina.o.he ,...."eri.y of 

.he chronic innamma1ioo. IOCTCaliCin the IfllO\Int of aIKbidoruue wilh ronembenhip i. essentl.1 for suo;CeU. l"lIorouahly document the ~nl way of 

ope ....l;nl.he business I.!llhe ..... ,... point. Second. define specirIC.lly how thc 

bu,i"""s will pen.e wilh CIM. Nu., pn:part lhe dciailed pl.n for I""I"'"'sinl 

frum ""here you ere oow 10 where yOll 10'..,1 10 &0. Fin'Uy. u«u.e.he plan wilh 

capable lcadc:r>-hip. The .pecific de •• ils for developina and Implemenlins (m..,asinll 

,be CIM pro;":1 will be aiven in.he presenlalion. 

INFORM. Vol. 4. no. 4 (AprillQ93)

486 

Session I Monday Afternoon 

New Surfactant Technologies 

Sponsored by the AOeS Surfactants and Detergents 

Division 

~panlloa aAd Properties of [tho", ....", "IUy Melh,. Estrn Non6onks. I.,..., 

Hama. Hirofumi Nabm"", .00 HiroW NabDo. Uon COll were ill¥CSliplcd. Thes.o. mataials wert' found to be 

COfl'IplNly and rapOdly INodqradabie in mvironmerual matrices. They 1l'm!"'50 

hl.... y {>9S1Io, momY~ in lC'Ii.-.edsludgc: Iype KW1Igc:IrCIImmL Hcna:, biodcgntdII;on 

ill meanin&ful mncwaI rnec:hanism for thc$e: 5Ulf1Clatl1l in thf mvil"CJlltMnl. 

A biodcpwJation pal.hW1lyfor the material "" poUUlaled and found CXlftSiWlnl wilh 

the Iypes 0( I.......... metabolites formed durinl biodoaradat;on Ie$IJ. The aculc and 

ehronic: ","ldly of IhlllUrflClanl homolog oeries 10 ~i"c spcc:ies of three 

aqual;'; If'09hic: lc¥cb (PjMrpltaltl ~ku. DapJuriQ INlgfIQ. :kltfIQSf""" fGPri- 

""'~I/II'M) ...-as "'10 invotiplcd. The r~ or !hi. diJCUMion will be on the environ. 

malI.tArtty UR"mefll for lhe oquatic compartmem for lhe C 12/14 (Coconut) 

derived mll~rial. Toxicily .... f""nd 10 be "'kyl-chlinlcngth relaled in the parabolic: 

fashion which il tlOf1I1 ... ly ICCII wilh ... 1 SUrfflCWllS.The ........ oensitivc specittt 

among the Ihree luted wq the invcnebralC DI>p~ltiQ INlgnu wilh. mc:.,..~chron, 

ic no-ctfcct·co.lCt'mmion of 4.3 m&IL A di5lrlbution ofanlkipaledconccntntion.s 

in United Stal"" mix ins zones and rivcrs we-re obtained using PGGRIDS and 

!'GROUT AIipOClively. 110'0 n~lionwi

[C.oH21OCH:zCH(OCH;t:'OONa)CH2J2Y, hete Y;I O(CH:zCH~)". wim x'" O. 

I, 2 ... 3, wen: prq>u'Cd f""" diepoxy earn Thrir surr---.-u"., pnpcnia in 

alkaline ~ lIOIulion were ~.1IIId Ihc: .uuhs wen: ocmpue1 with 1be 

dMa !XI Jimi'-1)'pCI of doubIc-chain swf...untJ bewina; odItt (two) icric bead 

poupa. lbrc dfea of the ditfemn in be:Id poup. ns of ph-= equ.ilibria have bmI ~pIetcd u.sin& sevavJ ethyl",,", 

o;u.ide/b.,nylnm 0J.lUe o~idalively onore $Iable aU 

cis oils by blendin8 monoul1SlllU.. 1t

488 

~ \'arId} of bopnnoid Elbn- Con Upld5 1ft ElirtmelJ ThermoKldophllt 

An:hlldtKleria ~Ionglng 10 Sulfolobus. Akilliko Supl, Il;uko Fllkudl.. Ric Saku. 

ma and Tosbihim holt, K.iIaS&lOUniver5ily. 1-15-1 Ki_o, SapmihanHhi. KaI'IIp_ 

Z28.Ja!*" and YukoH. [\Oh, Sob Univ=ily.J .... 

Moo ~ lipMis ofSulfoiobus IlIl' ",,",posed ofletrwther con: lipicnt.1 high concmt ... ions. "'hik okk.linolcic:and 

llIlIChidonic: UltS8fUt3ltd fauy lIoCids ....en: found~,low conco:mra.io .... Free 

cholesterol conlml in boIh fraction. withoul deminenlliwion ...... high. F_ ra.. y 

ocid.....".., present .. high ]evels in boIh f_,ions with demi""nlIiulion.. 

An"I,.,., oI'TriHylglyttrol by HI,h.puror"",,," Liquid Ch ......... togr.phy .. ·lth 

Ught·SnttltfinC Oti:""or. Zhipi". Goo. MuOIO Fult'e liquid chn>maIography {lIPLCj mcchod ror com· 

mcrcW lodthin. In thi~51OOy.phosfWIidykboline. pboipIuIidylClhanolanline. pr.. 

phalid~lino)l;iIoI Mel phosphatidic: ocid CClnlmts of IO)'bean and cII yolk Ioc:ithin~ 

were delennined. A "lica gel cohonm for seP"lalion and "bJorpIion .. 206 nm ...... 

u..ed (or peak dClcaion. Cornmcrci.llecithiru; "'hose pholipholipid eonle,.,1 wu 

dctenninW by 11..C wen: used as SIa.nd.miS. The rcspoo.'" ofphoopholipid on ult",violc. 

dot«tor l"'Iely 

il 15 presumed th;lI these phoInomeM c:auICd by fn:e ndkab,.,....,..ed III """.he 

_, .... i5",lf """lear. To Vi'riry ..._ r..:.. radic:lds ~ the w.idou,¥c dIom- 

"'" iIvo the nervous ~ _ "'hC!her witamin E p«III«IJ it qain.r: an o~odaIiY., 

~ !he clfca of oxygen on !he function!; of tlCJVr ,,,,,,,inalme-rnbraneJ in ,.. and 

the pn:Mect,ve effect of,· ...... in E IIIovcbttn ;"'"t5Iiglled. SynaplOSOfltC:l (1OOlFisch· 

.,.,.]44 .......... ich wen: eI~ by 100'1>o~)"gcn aImOlipbcrc al20'C for48 h....".., 

isotated. 1M membnonil' penncabilily III glllCOSeand.he me-rnbnmc nuidity we",

incruKd oi&nifiandy. 1'he miobubi,w ..sol lUCIive w~ val""", oK $)'napI- 

_ ""'"' highel-Ihan Ihose of unuptraIin' hiaher f= fA 

_ in pIastnI). The rllKlin& thai branC"hed fAs ha~ an especially ctrlcicnl 

poItIItlaIinI rifen ... PMA-«tiVlllCd PKC is or particular iDIercsI: mri shows a .w 

IJIPIU.IdI of !heir bicJIoP:aI role.. TWo imporUnl--oanu.clusi---.......,.. or fA-tnedi- 

IIIcd rqulltion Qn lit: prtIp

490 

muoosa by h)'dropilobK' ...cI affinity ~ and ... "" """'n it 10 co.... ;n 

ICyl CoA lipoe.1ICy1 CoA h)'drolue lflii mono- and dilocyi.iyttruI.:ylnansferua.. 

TM .:)'1 eM hydrol_ was moIvod frnm Ilw: romplu in. pun: SUII' and ..... 

found 10 form upon ;"",ublllion with .ql-eoA ~ covalI'm Kyl'proIein bond lhrouSh 

I high energy lh~s'er. which appeared '" be R!(juin:d rOf IICyl,l)'nd tile tole or STPs in CllfCinogcnesi, toM i15amenabilily 10 

ditWy ID(ldui1iion ....... Id be of tbtrapeutic importarltt. Tho:f\'fcn.the main 

objective of t.ho: pracnI Audy ..... to asocu I"" effect of. Iow_fll di

~ .... ilff«ll:d by the..,,1 donon.. orpnioc :soIV~IS. 1aII~ wale< 

eont~t. pB memory ....:I immobiJizaIion mcfhods of au:ymeI. The anhydrous 

(Iyophiliud) enzymt" and r.. ,y ....id anhydrides """~ Ilea for rnonIICQer producIioo. 

The: opIimum pH mll" wa'e 4-S and 11-10. The mllll;mum yield ofpnJJlyJ~ &Jrtol 

monoiaul'1IIe. monomyri~e. monapaImiUlte. ITIQI'KloSrcanue and monooleote with 

SO mM fauy a.cid anhydrideJ u ..:yl doooR was 97.2. 79.6. 83,7. 89.7 and 9JA mM, 

mptetively;!hal with SO mM falty.ods u ..,yl donan ...... 37.3, 28.7. 28.1. 3S.J 

and 36.2 mM. resp«tivdy. The muimum yield of propylme' &lycolllDXlpllmitllC. 

__ and rnanooIeMl: ",ith SO mM uigl~ .. .:yl donan ... !7.•, 

6S.1 MIl 83.2 mM. mpecli",ly. 

... 

SI~lK1h'f BIon.>n.u:dGn oI'Orpn.osllkon Compounds by Upases. Au.oo 

1lInab. To!;hiaki Fukui and Tlkoo Kawamoto, Depanmem 0( JndLrnrial O>emiwy. 

Faculty of Enginmin8. Kyom University. Yoshida. Sakyo-tu, Kyoto 6O(H)1. Jopen. 

Non.naJuraI orpnic compoundil have bcm br:comi"l imponam as substnues in 

bOouof soy-based food., IIItitude!; .oward 

food~ wilh!iQy ingf1!dien" on.hc label. food shopping prw:lices and demog"'phic5 

ofre.;ponw,ms. Re:spn!ICfn:qo;elocles for each queslion and chi-5quare stali .. ics for 

....... ·Iabul.,ions ofan~wen to !lClcc:ledqueslions ....i'h .ge. income:. education and 

are. of residence of rcspondtnu were calcul~ted. Drda indiaued ...... oonsunw;o; Ilad 

minimallno"dcdge abool.wy foods and .wy-derived food illJf1!dir:nls. ...... lhe f~ 

queney of """if,"" of difl'~I.wy...:on .. ining foods varied and the likelihood lhal 

they ....ould u"" foods ...i,h ~pcfifk liD)' ingrr:dir:nt IWIITIQ liotcd on a food labol ....... 

10.... Answe ... 10 optn..ftl(\ed ql>e$lion. =ealcd .... ( respon

492 

~in moIlly Ihmugh ~ hydnnyl B"OUPof -me n:sidue. ... the (-amino group of 

Iysi"" residue. Bolli lUClions causc an inc:rease in Ilegluive clwJe "';thoUI signlficandy 

.hering lhe originllltruc1u", of the prooe.n. VariOUJ en~rmn' have been ~scd 

to l and e"'yl"""alycol 

wllS.ani«! 00. 10 know whahcr the 5OIubili.y in sallW ..... erc:oold be reoovcred. 

Redurina agenu wr:n: eff«live '0 revert .he 50lubililY of Ihe .",,,ed SP!! in saI.ed 

willer. 5OS·PAGE WIIIIysi. of the ~uble proceln oC SPI in the presence and .tana: 

of oil. indicated Ilw wi'" the dcaa..c of JOtubilil)' in IAhcd WIller. W MOIIIIlUof W 

t.sOc: .MIbunits of &1yrinin in the 0II1Cd _ beo;ameo Iowfto IlIat! !hal in _. The 

me SH CIDfIICIa docreaKd in !he ~kl fll$hion. lbeK raults IlUgalaldw the 

~ of JOtubilily durilll SlOIqC __ caused: by !he o...idllion IUdI III disulfide 

fonnalion beiWOl!llIhe bMic .MIbuni. poIypepides of ,Iyo;illill. III flC!. whm SPI ..... 

stored under N2 &111 i~.ado"i •• or whm Ihe fm: SH &fOUl" 0( SPI wr:n: modified 

wim iodoace!ie Kid. the o:Ioa-eIISe of !IOIubilil)' of Sf! ;11Ailed wI.er was eff«lively 

pn:venled. 

". 

Clinollo PnMeins. fen:idoon Shahidi. Memorial Univmily of Newfoundland. Dqw.. 

of Bioo;hcmisuy. St. John· ... NF. Canada A IB 3X9. and Marian NIICZk.DqIanmcnI 

of Nucri.ion and Conoumer Studies. St. frMcis Xayja- UniYcniIY. AntipIis/l. NS, 

c.n.da B2G ICD. 

c.r.oI. ~ Ihe _ ;mponanll oil......! crop in c.n.da -' IIIIIb Ihitd in lOla! 

world prodIxtion. II coruino I R-24'*' P""C1nli wllich &tier oil ulrlClion ~hs in • 

meal possessi,,& .ppo~imarely 4(1'1, pn:Mein. The meal proIein I.rcaaonably 

bIolanc>edin its .... ino Kid """'posilion. Howev«. u~.ed ule of canoIa mal in 

animal feed or poosibly In human food foonula.ions hIlS been .hwlUled due,o!he 

pRRnce of an.inulri." ... principally glucosinolol5. phyWl5. phenolics and fibe •. In 

.deIi.i"" 10 studie. on lhe5c amlnu.ri ....I•. I number of poroceuea; for "''';r I"CIIM>V1Il 

and uparading of Ihe mul have bten reponed in Ihe lileratu",. most 0( which 

involve solvent utrw;1ion. or these. proc:c5SQ involvina: alUno/·ammoni • .-.d ~. 

QUI iWJaaion have been ""'"' effective. F~ pMCin dcriYMizaUon by KY' 

Iarion IIu rcsullO~d in me production 0( funcUonaI proICin illlfCdicra. The ~ 

meals 50 obuincd .... y off" ~ for incDrpanDan inIo fonnuWed fcxdL 

Session 0 Monday Afternoon 

Nutritional and Utilization of Vegetable 

Proteins In Aquaculture Diets I 

Sponsored by the AOCS Protein and Co-Products Division 

01 

The s.:. or Aqlllallhl~: Clln'ml Sial."'" FIIIIIl't I'f'obI,cIft!l aDd Opportll' 

nilles. Robert R. Sridrq, School or Fi$hrrics. Univcnil)' of WadtingIon. SemIc. 

Wa$hi1lJUln 98195. 

Aquacull= ;, dcflned .. Ihe ~a of aqualic orpniAm. under c:onlrolled or 

ocmi-conlrOllcd condilioru. 1bc di..,ipline includes the re.ori.,. ofboth planl • ....,h .. 

ICaweed3 and animal •• uo:h .. Ii......mollu.b and ""'''''''''''''. Objective. of!"" 

(ulluri$1 mil' be 10 produo:c food for human con.umption. enhll>CC recrealional or 

commercial fisheri .... lupply bIoilor provide fi"" for the aquarium hobbyist. Aqua· 

cuJwre is responced proIein; howev ••. daily reet!inalllle influeno;es .... jIIKlSC.O dlffrn:nt 

proIein levels in the feed. Fish meal cm be 8",ally reduo:ed. Ind amino-Kid der,dem 

protein lOIIn:es.like colI""sced mal. (111be eff«lively supplemented wlth i)'llthe!· 

ie ly.inc;1I commctcial fcedi. 

OJ 

ClllnlltlAMS In Sal-'d NlIlriUon. RonaldW. Hardy. NOAA. NMFS. 

NWl'SC. UtiIizMion RacardI DivWon. 272.5 ~ Boule\'trd &s!, Scank, 

WtihillJUln 98112.. 

S.lmooid *lUICUl1ure incllllles the rearing 0( juvenile PKirlC and AILamie 

... lmoo in fre$ltwlt" for .nhanccmcnt pul"Jl'O'C'land for 510ckina ;nto marine Brow· 

001 pens. rearing ofposl·juvenilc ... Imon 10 markel sizes in JC!IWlle•. and "·0111 farm· 

ina;n f",""walc,. Feeds for juvenile ... Imoll must suppan ",pid I"'wth.o.he ,iu II 

hau:he'1' rele .... or ..,awll .. trWlSfer. Nutrilional i$sues for juvenile rurlnalnclude 

producing low·pollIHion fcedi whJle ensuring optimum nutrllional SWill ol.lle 

y

40 kJ a..mb.bIe ener&Yf pnlICin. Their diHar}' lyoi.....~i""""'" for maximum 

powth and feed efficiency quamifocd 10 be I.5S'Aoofcby ditly there. is • lack of specir", 

infonnalion ercial Absbn 

poIloct. swimi. 

493 

'" 

J'tIysIc8l CharKlft"bt1a: flIClllbh Surlml ~ .. ilb MilIR' Reco\'H"KI 

r..... RIkt fnmes. lin M. Kim. SIeve Liu. long B. Eun. ilIvid Veal. Mit...,1 J.... 

ne" and J_ HearnsbnJcr. Mipj";!'I'i SIa1e Uniyersily. CoasIal ReseurlI and 

ultft[;ion Center. 3411 Fredtric Slreet. f'uca&oula, Mississippi 39567. 

World demand for swimi CClntinUC$'0 pow. and 1II:W species hl.ve becro 

eJplon:d for the potrntial surimi p-oduction. Surimi ...... pn:pan:d with mintc:d cat· 

fish..,.,,;,vera! from frameJ after filletin •. The deboned "",ill! was...-.!ohed eitheronc:. 

t....o or Ihree limes with .....Ier for ... rimi procM$ing. Unwashed mince was also 

blended ....ilh cryop1"OI«1lU1 for IOIlrlmiprocessin&-- Heal·induced gcls \Yen: "",pared 

usinS ei!her washed or unwashed wli'" ...rimi. with or without .wclI. "The f'l'SUlu 

indicafe tIw then! wen: no PlJlifkanl diffen:nces in Itl

494 

IJiokI&ically oct;"" &lm:>MIs..nd thio hH hem III impcIVI in de\lelopin& foci .. maIIod:I 

for chie;rprepanulon. Then has hem considmoble In!~ in reidallkpoaicb. A mix!",", ofKMn04-CuS04 in ",nux;n, methylene 

chloride. in ,he. p.HtflCc of. cuaI)'Iic amount of WlI~r III1d ,olIphatidylinosilol (0.4'.11»"'ere lo",eml. 

The moaI abundanl racly ..dd in PE was 18: I 1-40'1.); in PC and SPtll6:O 1-60'1. 

eK!I) _ moll abundanl. Ow dac.a suggest iliac lipKe diff.mlliacion ocaon in che 

PI' juncti0n5 01 bovine lms fiber cells ond iliac ~ mIIY be .. opcciaI requm,mau 

r.,..choIeSICIuI in OJ ~ PE and SPH an: ee IWO _ pI'OnIinem claucs 01 

phoIphoIiplds ~ willi ttt.. OJ mriched mernbnne fnctioIl. 

es 

Sepa .. 11on oICOI\Iuplrei OIe ..... F.ny Adds from Dln"trttu Soun:a In Anlm.] 

.nd Human T"bsuts. Sebtlstiaoo 8anni. Mari. AYunta [)asl. Maria Paola Mel•• 

and FnncelCO P. Comn,iu, Dip. 8;0Io,i. SperimenUlle·Se~. Pllolngi. Sper .. ViQlc 

R.,ina Mnrghcrila. 45 09100o..giiari, Italy. 

Tht! pracnc:e Ofronjll'Bled diCOC$(CD) in lipid .nvi .......m.m Iw been lliken for 

yean! u ."idenee of lipid penuidalion due 10 ee cceerreoce ofilli. 51~lIre in flllty 

acid h)'droptn:>~jdel. Small amnunts of CD filly acid i..,....,n an: aI~formed during 

partial hydrusenation of fIlS.. A ... consequence. chey an: prucnt in diets prepared 

with partially hydn:>aena1CldfllU. M(II'llOVa",il has bcen ....... 1hat co.fany.,id iM>men 

art ab50rbed ond dqIoIiIcd in animal MIl hIInIan liuuell. Hence. due 10 !he . 

CI8:)n·3: 0.7", C20:40-6; 0.1'.11>.C20:6o-3: 0.]'.11».The ..im oflhis study "'1510 

verify iflhis fll CQrnpct-ition and panicul ... ly Mer could Iff tel mcmlnne ionlldlain 

poI)'lWalwatcd r.IlY acids (PUfA). Since red blood ""II (R8Cj mcmbnno. 

PUFA reflecled brain membrantCQrnposilinll. _1UIdiCd by pI·liquid ch.romaIOJraphy 

OLe -')'IiI (capillary column CP Sil 88 SOm ~ 0.23) in .. multicentric 

study. filly acid """'J'OJiIions of RBC phoIspiIaIidylethanolamine (PE)in 1_ poup. 

oIprenw_ inf .... 

IS96.t n; OA:)2 

: Group A fed In HM + InAl..FARE(n. 12.,...,.,·_;8W: 

...b .. I); Group 8 fed HM ("'ilh.ool lipick modifocacion. only 

IlUpplcmcnled ",ilh CltJcin hydrolywc. minerlband du.1rinc-ma/105C!) (n. II. BW; 

16J81 ± n; 0....... : 31 ± I ",k). The weigh'pin .....,CQrnpantble in the t_ ,"",ps 

IGroup A: 16.2 &lkgld: Group 8: 14.2 gIkgId). The PUFA CQrnpolilinn In Group A 

in dlCIC PE did not demo:>mIrate any imporwtl nuauation, and lhere wllSll'l any 

IUIOOUIlyfor C20:)n.9 ond CI6:1n-1. AIIhc 37th wk of .... !he two JIOOPS 1II'CI"I' 

comparable. In oonclutlon HM fac modifocacion "'ith ALFAR~did not diMurb 

mc:mbrane:s' 1ont~lWn PUFA. 

Compa .. U"'SludyolSp/llngocny~Un Filly Addlln Er)'lhO"OC"Jln or Prtmalu", 

Inflnu Frel wllh Mllernal Milk. Formula or a-Unolenk Acid Enriched formula. 

F. B.bin and 8.lHlCOIrIps.INSERM U..58. 60 rue de Nlv.,dlcs. 34090 

Montpellier. P. SInb and D. Ricu. N6onaut1ogic. A. Craloln de Paule!. Biochimie A. 

and F. Mmdy, OnIdol,C~liGm. Paris. FI"IIICe. 

The essenlialily 01 n-3 fany ..-ids: Iw been ""lemivtly documented and .. ve 

rUe 10 llUdies 01 «-linolenace supplcmenlacion in formula f«

chromalography.1n RBC Sm.. li",irlcanI rilOO""lI oboervcd in the pen:a!l.I.Jeof 

24:1 o.g (ncrvonic acid) between 31st and 37111weelt. and \his rise wu compcns.llCd 

by • decline in 16:0 and 18:0. AlIhI: 3151 ond 37111weeki' ,e5tllional &&C, the main 

fauy Kids (...) wen: rupeaivcly: 16:0.37.3 :1:1.3and ljJ:I: 1.4: 111.0. 17.6:1: 0.6 

end 15.7:1:0.1(I' < 0.05): 24:0. rs.a to.9 ond IUt 1.0; 22:1 e-s B al_ys < 0.1: 

24:1 n·9_ 9.9:t 0.07 ancII2.8:1: 0.9 (I' olYffl f*tIilion in btik ond acidic enyiron· 

menu ....ith elhyl atelile and mc.hylenuhloridc. ckrivllliulion 10 I.perufluorilenlyl 

e:51e1 followed by Ih.in-Iayn chronw.,.,.p.y. mc:1ho~imalion mel Dimelhybilyl.lion. 

QwonliflCllion .... Khievcd by capillary .... chromalOJl1lPhy-e1ectrun capCUrc 

Illndom _ ~rommy with I Flnni .... ·MAT 1'50-708 triple ~ insl/'Umen. 

opmIled in the IICgative ion delmion modi' ....Id! meI~Q IoniuIion pi. The 

mean inte ...... y coetrocienl "harialion _ 411'lo ( ..... se 0.4-10.5) eN,. 12). The 

CJlCretion of POI2-M in 27 .... llIIy male lubjeeu on lcontrolled basal diet (aga 26 

1O!i7) was 164 ± 68 (mean ± SO) ngI2. h ISdelctmincd in n-h urine pool .. 

PB 

495 

OIelary Fal Modulates thl! Synthesi!l oI'Thrombou"" Al' AIdn Fe=tti. Joseph 

T. JIIdd, ~ P. Nair and Vmcenl P. Flanqan. USDA-ARS-8HNRC. Rm. 

122. 81dg. 308. BARC-East. 8ellSyillc. M,lfYiand 20'70S-2350. and Philip R. Tlylor. 

OHHS. NIH. NO. Bethesda. M,lfYIand. 

The n:sulu or decades of I'CIl'aR:h I\lga.I th.ot .he currenl epidemic of diseases 

of the tIniiovascu .... systan in industriatiud oociaietl could be allevialcd by pmper 

dielary chan..,.. For eumple. pDplIwionllUbWlinJ larit'ly on marine food u.hibil 

reduced rnortafuy from coronary UlIU)' disc .... Thit .. udy iI.oomribullon -.m. 

tlarify .... the underIrini mec: ............. The ~h .... 10 demonsa1Uc the IbiliJ)l 

of diewy fi.b oil 10 ....... Iale u. mo production of Ihronlbo:ur. (TXA2) •• 

I\IbsImce !hat induces formation of blood clou. In • IongiIUdinal srudy. 3.'1 lillie 1\Ibjetlscomumcd. 

controlled '-III die! r.. l uperimenl&l periods Wain( 20 

wuu. During period I (10 WC

496 

Session E-1 Tuesday Morning 

Analy1ical Techniquesflnstrumentation 

SurfJottIll\! AnalysisbylUlh·Pur ... ma ..... Liquid Chromalop'1lphy UsinIC11w: 

E.-.pontj.e Lltht-Stslt~rlnc DdKtor (Booth Sl.'l~T. F...... v.n Cocp. 

~ Ad..._ for 1MMtNISPt:Clow AtId N...,lnr M~ Jite§On• ....., Proct.'lII 

Anal,Uf"-AppliQol!ooos for Solid Fat Conltflt "nII','511 (Booths liS and 

117), Htnfy J. Stronb. L Sp.WDnI and Q. Bulow, Bruh-r Spec:tr05pin (CanadI) 

lId.. S5S Steeles AvmdII! Eo¥. Millon. Oniwio L9T I Y6. Canada. and W. BUIll. 

8",", Analytiocht MesoIechnik GmbH. 

Stuker C.... will be p-e5ft'IIinr; infOfmlllian on ra=I ~ IIIIvw>cQ 10 

~ MINISPEC NMR IJRlCCS' anaIyuJ:. Thaor: newcIopmcnH will inaase!be 

eMC of ill5lf\lonmt openIion lind will provide • IlIf'KIlIIO:Ihod ro. measurin& bod! !he 

lUIid fal tonIaII and ~ .... IWI oil roNent in ...m. and food products. 

EI.) 

1M \'_tilil)" olin E~aponlh'eU&hHlullm"l Oeift1or(Booth 103), 

Richard M. DnI'RaUll and Thomas N. Villawnar. Ridw'dScimfiroc Inc .• ~ Ikl 

Muin keys Blvd.. D3. Nov:uo. California 94949. 

A rngcrwion. (ii) 

combine hi", ""ill,,"" ... iM poilIom: with hiah IClivily and selectivily. (iii) 

...., m«lwlic.Uy ilablc!O C11$UreJOod fillenbili.y and (iv) ~ easily be rccovaM 

Ind wort:ed up wMn &pen t, The lim wu ICh~ved with. Cllalyst of........n:_ 

IIbly simple compo$llion. i.t.. nicb-lJmagncsi. on siliC"l, bul with. can:fully 

desip1Cd ill"UCtun:. A. opct;ial _Ihod of !'ftCip;IIUon is used 10 yit:ld. cataly" 

with llarJe mclIIllic nickel wrfla....,. within pores of. IWmw disoi.bulion. 

&.ampIc:J rill"lhe prrl"onnancc o(r"" CIIalysf in various hydrogcnuions...., 1'"'_ 

~... 

Session E-3 Tuesday Morning 

USDA-AARC 

TH A.A.RC Cen,...--Indll!lrilol ProdUdS from AI Ma'niaIs (Booth 336). Joocph 

C. Rocl""';' USDA.·AARC Cenr ......)42 Acn>spKc Ccnlrl'. 14th a IfIdcpc ..de",,~ 

A."".. SW. Wlshing'on. DC ~2200.

The -'ARC CaMcr G • "" .. indepcndenlOllilY in !he URiit'd SlaW< Dqwunml 

of AgricultllR>. The Board 0( Din!cun, oppoinIed by !he Sc=wy. arc ~bIe 

r.... policiQ MIl propam. Se~en of !he nine boNlI rt1

498 

Company. IOJS W. GA.'mI! SI.. Piqua. Ohio 45356. 

Frmch .. HI dillWH it. complete line oipCI'" wllh ROBS 

1hai pmvidQ the """'I)"s lowe:$! toIvenll'lem lind MJUST IN TIME" 

flIW~I i.,vCftlory control. Finally. lhe 0051of""".non! ....omation i.quid:ly 

tuChinll" It:vcl "'hldl il aoc«pUble for eYU>the smallest IIneI.. Colcam heaiinl 01dcodoriun Is the standard ~ of deodoriur 

hea!ina in Europe and many munuics. In the Uniled Swcs. _ dtodoriurs lOCIIy 

Sill! '"' beaIN by D;""lhmIl. Ho-o.~. il appem uif the indlutry in !be U.,iltd 

SWco _III"" is be~nlllO i0oi< IIhip.JI""IMl'" Jttam heating. SevttaI nup 

comr-nies h;I~e SlarttdlO tvnvcn deodorilCn from DoIIo'lhnm 10 high_I""""'" 

Ouillcnno Sulbarul. PSI 1'rocQ,t Sy'tom'~. Inc:.. 1790 Kirby Parkway. Suit~ lOll. 

Memphis.Ten.- 38138. 

Tho! OI'(j\ImCc of C'V0IfIlS which W;" pboce for • JUotteS!;ful dnip or an tdible fIlS 

_ oill proccui", pIInI SI-U ""itb lite selection. FoIlowing"le .,kction.~llioo\s 

mu.t lIiO,ivm to the amounu _ diffttl:lll type:!' of oil. to be proo:essr:d. FiMi 

product miK and c:.pICily ...ilI delrnnine the size ond compJel1 ..... and hlJliI,arlo: Acids. C. William Hlewen. Uony Sallans and 

Oilbcfo H. V..:e. Henkel CorponliiooVEmcry Group. 4900 Estc A"enue. Cincinnali. 

Ohio4.52.32. 

Infomwion .... ilable in lhe LtICl1lIU",on !he"""'IU", of dimcr and iS05learic 

I('ich: will be«>ll'~ 10 IIuII obIlIincd from desorplilissan OirdJerC.II' 

Iyil Co. Lid" 9·2 Kondo.lacho z-cncme, Chiyooa·ku. Tokyo. hplln. 

Fi~h oil is ooc of I"" "ilul row malerioJ 0QU1'Ull for mlllJlarine will "",,",cning in 

Japan. 1bcre an: I fl'.w other toUmri.., ulilil.ing fisll oil as an edible oil. The produc. 

Ii"" of.,.!m flny acid mainly in MoJ.y.i. and Indonesia i. iroro:as;ng. and ",,1m 

f.ny I('id hal cmerged as a principal olcoctlcmicoJ feed. When fi... oil Of palm fany 

acid is hydm&m11ed. pcrformllll« ""IU;"' ......IIISor lhe ca",lyse differ depending on 

Iht requln:d pmpcnics or OIherc!cmenls of I"" nnal producL Fisl\ oil and palm fany 

acid hydrogcl\lIlioo TCSIS"'cn: cono.illl:led usinl nickel camiyS!' coaled wi!h oJkali Of 

alkaline canh.> in conccnlfll1~ of O.OS-.5%. The ra.ultanl hydrogenation ctw.:lcril;IU 

vllficodwilh lhe types and lmoolll. of alkali or .Ikali caMs applied. 

Q7 

Salunllon of ~-atty Acids In ~'~ed·1kd R.acto;Jf'$. F~ Bominkhof. Engelhard De 

Mccm H.V.. P.O. 80~ 9.)4)4 ZO DI: Mecm. The r>ltlhedwm. 

The h)'droscnation 0( UMl!W1IlCd rally ocicb 10 iIIIUralb:i fOllY acids is LISOally 

carried ow in a >l1lfTy phase procell. Since in mosc (:a$' 

cia"" ICSIIII ....,11II in • rued·bed I

500 

SynlhtsIJ 01 PoIyuruaturaltd Fan), Aminf$. Shiaco llIkeuchl, Setsuko H.... and 

Yo;mim Tocani, Faculty 01 en.i..-; .... Seik~ Un;veBily. ).1 Kichijoji·tiwrudli 3 

Musuhino-ihi. Tokyo ISO. Japan. 

C\mmtly. the fany lICiIh:IlKd III no,.. materials for indll$lriaJ fatty aminell)'ll- 

Ihcsis,,", a1mo&1 all &alunlied or monomoic Kids. II is upected Ilwol poIyunaturliled 

fany .... ina would ~ availlble as noW materials for new maslr.in,lIuffs if thoy 

"""Id be ~ simply and with JOOd yidd, H they haw novel funclions. $UCh .. 

• brocr ckJin& propcny com~ willi \as ~ '...y .... ine$.. Inthe ~ 

wort.. the opIimwn lUClion conditions wm: cumincd for poI)'\IIIcd from itJ intcracrion with rollon. Qual depot.!lII --u.s 10 itJ hydrophobicity. 

10 an CJtICftl dcpendin, on tlte fabric wrf"", area. Baidts ;tslitde .cndmcy 10 

",.um inlo soIu.ion. D11>MAC h;nd!lIOCOIIOn by London diJIICnian fon:u: I 

Coulomhic inleraclion OCCUl'"S only when the fiberhca" I M8u1ive charge. 

Ttdlldcal Sltps In .'ormll"ling eo.-nlnltd Liq ..1dFabrk Sof'Ientn. Nancy 

L Johns .... and BartIMa I. CaJIiu\. W;~ CorponDon.. YTT1 FTana ROIId. 

Dublin. Ohio430ll. 

Conctntrated flbric' IOfiCllCB an he fonnula>cd which "-' !be chanpnl mar· 

kIM req .. irancnlll f)I( lho. manufKlUl'n" willie ~in, .he q ... lilY consumer. «pea. 

MIIIUfKlIImS want hiJh ~id:!t fabric ....rtenm wI\i.ch Iuov. low visc:mity. uecUetll 

pcrform.ano;:c ~ impnIYCII biodtp-adaQon and _ic:oI ..... rrwcrials. 

This JIudy WIll daipcd .0 ~ the chan,;n, needs f)I( mlUlllflCllIttfI who WIllI 

.0 provide novel physical dw..:.eri .. lc.llooJ!with hip lluali.~ pcrfunnance In f.t>ric 

wfiellC1S.. Ne ....formulalions for conttnltllted fabric wftmen wen' developed 

uling nontraditional IIIW malerials and mbing l.dmiqu

Ever _ina: r.brie IOr1(r",~ wc", 'n;llolly developed for. $pCCirocpIIlJ>OOe, \he)' 

ha~ been n:desi,ned and adapted in ~!IC \0 changing requireTl'lCnl$, No maner 

"'hill tht:!IC I'aIllin:menlS loo«;pha1..,IIC!~ aR! w~ll_known namc mllfdlUlls ror COl- 

IOn. Fabrics finillhed wilh lhese ~all~nI' have phosphorous finnly bound to the cel· 

lulo5e bul_ ~bIc 10 ...... ofnitrOl

502 

methods ISO 66S (rroillure and volOllle milner, and ISO 6~ (oil conl.ent) 1anmau 

and Biodomtical en,............ L.ondoa. Ontario. N6A 589, c.....sa.. 

Low- or nepliv~ lIIb!.Inu. is I prerequime for cornrn=ial 

01 0wmi'IlIy 

~ion or 

biolwJrflCWllS (Jiyoolipidl). CanoIl oil roukl be """ 511C'hsobsmuc becaUIC 0( its 

plemirul ... pply ...... Iow cot.I.lllthis work, T",,"/npJi.J bombkolD is used for pod",,_ 

lion 0( &I)'OOIipidl(1Ophorote lipids) ~"Il canoIl niland glucose: Q carbon IOIIrttS 

and o.hakc nasb for 6-a dI)'I1I 2.SO fJII1I ond JO'e. II ..... round thai die jWduction 

0( &i)'OOlipMh DDUId n:am IJO-ISO ...... in die medium .....,.;m"gof 10'It &i1lt"OOle. 

10..5'.110 ~ nil. 0.1" ilia Md 0.3S-O.4')l. yea>! utnn. AbouI.13'.11oof...... apoI.- 

IOphoruIc lipid 1'-4"_1IC\one 6·.6~-dixcwe (Slol) ..... found ill the mi~lUreol 

IOpIIon>oc lipids ~ UIIdc:r these conditions. UK of canob oil .... n::suilftl in 

illaeucd y;.ld of fOJII'tor

(PC) hydrolysis in orgMlc Sedby 26 of 36 """""1iB (p .. 0.0001) 

Indiralinilhal clII"ItIC did impan a navor 101M oil. I" a second ~Iudy.canola and 

$Oybean oils wilh andwilhoul citric acid 000 ppm in propylcIICglyc:ol) wc~ ...on:d 

III 6O'C for 16d Trained panelists raled the oil~ronlaining cilriC acid higher for 

paimy and less for bullery odors lhan oilsconlaining no addillve. This WDSl!Ipc!CiaJ. 

ly 111.1

504 

arilinal_ buner. AI ay5IalliwiQn ~ crysWs incRa$ed in Ilw:ir pm. 

pof1ions or 50S in !he lriadL)TR N. 

Thomu WIdCorneli ... VenI«a. food R~ InoliMe. Wcnibce. JOJO Alnlnllia. 

The Slerrin or han! frw:tioo fn:lOl1a 5in&ie 51q> dry ftxtiooalion from anhydroul 

milk fll ..... blcnckd ... ith the ori,i",,1 anhydrous milt fll feablad: and ~I' 

cd i",o. daft cborolllc formulalion dwing """,ufatl..,..,. The anhydrou' milk fll 

and bl~nd5ofonhydrou$ milk fll and han! frxtion at IIIli.os of2;1, 1:1 and 1:2 were 

IKIdcd al I ronc:emllliion of 4" milk fll of the IOIar cbocolale m .. s prior ICIronch. 

ina. Following m,,"uflClure, rICh c'-olale was Icmper.llUre-(:ycled in "" lICttlclIIl. 

cd M(>nI,e ~Iudy 10 C .... IU.le fnl bl""", orabiliry. Vi.ual and o;olor measul'l!1lltmlS (I... _. 

b) of 1M cbocolalos formulAled wirh lhese four milk fnl prodUCUdellKll\Stnlled I 

iSMlf life Clf 14, 23. 23 and 29 d for the ""~ ofbllxming and lB. 26. 21 and 3J d for 

full bl""", development. mpecli.ely. Addil'OOIlI m.... urements mthe tempered 

cbocolalc Included ~ (fomo-def(ll"ntalion) and taSte cv.llIIUions. Resul15 

dem",,"""c 1M pIcnl'll benefil' of cOlnmcn:ial dry"fIIIc1ionated ""bydrou, milt fat 

in dIlrk cbocol.,c IIUl/1ufac:ture. 

t:rrH1 or Milk f.t t'ractlon51111 ral IlIoom In Dar\!; Clr«oIatt. Richard W. Hanel 

and My""3 Vi. 00:1". or Food Science. UniVCf"SilyofWiOCllMin. Madi$Oll, Wi5COQ' 

sin ~J706. 

Milk fll b known to inhibil fll blllDlll in cborol .. .,.. although the m«hllli5m for 

thiJ il unclear. One way 10 benerundmland bloom inhibilion by milt fir b 10 fIIIc· 

lionate milk fll and ",udy 1M dT«I$o/'....,b frvtion on blllDlll fonnacion in chocolate. 

In Ihi. OIudy• ..mydrou$ milk fll ..... di!iSCllvedin ac:etone (1;4 ~"OI) and prosreuivC!y 

frKIionaled It j'C increlll('ms from 25 100·C. A 1taIm5i~ crySlBliine 

frxtion1 and one O'C liquid f_lion WllSobt.olncd Melling poio\. thennal profik', 

llIOIidf., COIIIC"I.fally lOCk! rutd lrigly~tride profi!('$ we", measured for each milk fBI 

fRIClion. The milk fll f_li",,~ wen: IKIdcd 10 dart. chocolate al 2'1> ("'!wl add'li"" 

Icvd.ln addilion.lwCl contlt)l ciKl«>lale, were mado:. onc with 2.. (w!w) full mitk 

fal and tM DIhcr with 2 .. {w!wl addili"".1 cocoa bo.mer. An IICttlenired fat bloom 

1.,.1 WIOSpcrforrtled "" 1M c'-olale umpl('$: cyding cominuou,ly between 26.1·C 

for6 h and 1~.1·C for 6 h. FII bloom developn,ent was monill>le

tTyMaJliWion of simple lri&ly«rides (1'G1) has ~n dwDoterized at NSL.S. First. 

tiny ~'.11.(IritBurin) crystals, I.t..• bout ~Ain di.""",e ••foon briefly when tile initial 

cryitBIJ of (l-Tl melt. Punlbly becauSoethey.~ 5(1 liny. thne jJ' cryscal$lnlruI. 

fonn In 10 .... ';111 soIid,lOIid Il"IIIUilioo. to a \bird form,jl-n.. This r=Jlt is consis- 

'en' .. ith indirect ~~ from difremu:ia1 5CU>Ilinaalorimeuy but is ronlrary 10. 

re«nt npoft in \he: lit_lift, Second. nysulliulion of a.-11. and n-TP(lripalmitin) 

fmlll .... liquidswc hI"e brim rec:ordcd. ond the tMia kay" been ..wyud.. a...form 

of Melvin Avrami's kinetkJ. ba$ed on simple _mplionl of.....,1allon MIl 

aysul powth. is obeyed wilen jJ'-11. r""", dim:tJy from the melt. but not when Qn. 

forml. '!'hIlS Q.TlIT'P Cf)'IIaIJ IIW1 ofhttumuillini as t" wbelt' n ,. 3 moo 

than" 115for IJ'·n.. We """althat formalioo of «-TL can be faciliwe(! by 1'"'nillin, 

"""lei of higher ronns (jl'.!I) ralhtr limn IftIllirinllUlClea!ion directly 10a. 

Session W Tuesday Afternoon 

Nutrition and Utilization of Vegetable Proteins 

In Aquaculture Diets II 

Sponsored by the AOeS Protein and Co-Products Division 

w, 

Soybeton ~1r.1 H}'pft"lItMIth'lIy afId ErrK'l'l ... pffl"omwI«, Ni~ 

Mea.boIlsm., ItII",ultOlotkal RHpOme and Gastro!'"ell .... Hi§toIoQ rlRalt!. 

bow Trwl. G.L Rwnwy. -ruru- LalKnIory of FlY, Nlllrition. u.s. Fiih md 

Wildlife ~icc..1075 Grlcle Ro-d. Cortland. New Yortr: 13045. P.R. Bowsn-. Col· 

lesc: of ~LtriIW}' MaJidne. Cornen Uni~'y. and J.O. Eno.t=.. CcnUlll Soya. 

~ worldJUpply offish ml'al I. atagnaling ",hile the: aquacullun: feed industry 

;s powilll. Soy~an. "..hich c:omfi.u.eI.rout 75'1. of.n proIe;n f«:dswffs. ...m likely 

tor. one 01\he key proIetn IOUI'a'I for aquacuJuuw: f~ 01 the: finun:. Th= is. 

.... ~"eI. unpkevidcntt m. hiJh (>40'1.) dieury inl:I.,.;on level. 01 SO)'1I pmIein. 

isoniuosmousty and ~iaIIly ouboIiMCd for fish meal. =WI in diminiohed 

JI'O"'1h lilies ond elevllCd feed:II;" llllios when fed 10fi~ A ~ part of Ibis 

1"'01' performano:e has been .tribu,ed \O..,ybean aruinu.ri.ional fllClOrl;5UC'h .. prO> 

IeallC: inhibitOOland l~inJ. A sc:rkI of feeding ,rials and ,ests ...ith me,aboIism 

dwnbtrs hlvc been ron(hoc,ed ...herein sevCf1lIdiffcre!>' chemically and immullOologically 

defined soybean prepIIlItions .... hich had been ... bjecled towari""5 proeessina 

proeo. These include enhancanem of dip 

lion and -"'mil .. ion

506 

ulilily. QUlmilllivc ICMOI'y "vlh ... ion. """'" nocably lhe Spectrun1 Method. permi,s 

1ICe""'e and ",produeiblc eha.rocwiu.;o.,. ornu~row;oensory .nribulel "",rulin 

evllu',;nl prodUCts and, mo;>re Iml"'lflantly, in jdo,n,ifyinl the specifIC differences 

belWtc:l1prodUCIJ.ln our WOCed by 

5pimICh can be an effective I:OIIlrol of~ Insect peill. Intacl 5pinach a)Il.ains IhI: 

highcsllcvelsofinsect moll in, hormone in the apiealliJSUe (mgr', frWl) willi 

bio$ynIl!rJi, omurin, enlirely in lhe sublendi ... ,iUlla' prior 1

ttion oId>e 2.4.ll-dDubk bond. The homworms w=: rnrtd on .. artifICial die! 

concaininalS ppm 2.1ritio IL (l~of60 ppm} .... )30 ppm silOUerOi or wilb)](l 

ppm SI."",ero! (oomrols).IL.rompuaj ..... II.11e C"OIIlroI .. uumed ....... "tf>..lt:ngtb nI. 

3.S em ,,,.. 4.S em. i"t·fused ..... lenl ufSl of.1Ie 

.. eml mil.!u", "1. usee in .lIeron.roI .. "'pproximately 1.000000r IL WJS round.o be 

KCUmulaLcd by ..... inoea. and converted 10 lana;Iero! in 4.2,*, yickl2-Tmiol ........ _ 

lgreu lind prWuc. distribution. 

... Iso lhere. is • ne ....mochanism tor ,hoi 9P.19~yclopropyl 10 alke"" isomerization. 

...lIich indica.uone cll1yme may be re$pon~iblc f.,. the iruclt"Ollve~ of 

cYOOilIlCnoll(l rwtcol. I"",-,,",crol and loo.

508 

E~.11I811on oIr.l", 0I11'rud1H:b. Air H. Rl.dy. Nuclear Res. ecnu,r. AlDmic Ener· 

&y Authority. 147(A) Mi... &. Sudan SI., HlldAik EI·Kobba, Cairo. Egypt. and 

Mohamed A. Modkour, Mi!lr Gulf Oil Pmceuins Co .. 6. Maahad E1-Shasy.Heliopo- 

III, C.lro. EIYP'. 

Pllm oil product.{okin double ff'llttioNo!ed, &Iw. snonenilll.oo slearin) were 

evaillaled rCO'"Iboir ph~ .nd chemical propenieI.. flllty Kid profiles. umapunifi. 

.t>k mancr (UM) _in.ttu.1IIOOpheroI1IId IOOOIrimoI componmts. Phys;ro. 

chemical propoenia; or.,..", oil produo;tJ Wft'C.., foilows: 1.4Sn-I.4S87 refJXtivc 

indo. O.9072...(l.9241IJ'CCir..: p1!yity. 22-46'C melting poilll. 0.16-1.0 Kid vlII .... 

186-192 oaponirlCarion ... t.... 0-4 pero~ide "Ill .... 34-56 iodil'ltc val ... and 

O.24-0.49'io UM. Palmitic ""id wu the pmIominant "'tUllltcd tany Kid (35--44'1t). 

whileoleio:: Kid rq:.racmcd the majorulISIIUI1Itcd one (4Q...46'I,) follow«l by linole· 

ic lCid (9-11'10). Cia duumMography ...alysiJ or UM i1hlU.... ed!hal it CODIained 

14-611... h~ 14-)2'1>tocophnoI + 1rnpOIJilion ot hake liver ;0: 22.4'11 0( IIOlrUCd fany acids. ]2.8"" 0( 

rnCIrIOene!i ..., 41.'" of pol)'Cl'ft lot which 6.6'.1> iseicoAptnlaenoic Kid (EPA) 

II'Id 16.2'11 ildoroMhtltlllnDK':.cld (OHA)l.lod;'" val"" is 162 and SIpOIlifQlion 

val"" 183. Tho IV1'f1II" campolilioo of oil from hake vilOenl is: 21.9'11 salUralcd. 

36.6'" I1'IOr"IOl'rlt' and l4-''II polytnel (of which S.9'Io is EPA IIMd11.2'1: is OHA). 

lodiM val"" is 14111'1d saponifo ll.oll. bull! .Iye there was an irome1l5efrom 3-410 

.s-6'lIt. This did IlOl dfecl!he f~ly IICldromposilion of the ronain;ng lipid pbI!e. 

Unusull Gly~hlnlJOllpids 

CioemislI},. Klnk1 UnivenilY. 

In Marine AnImals. Akin ""yuhi. Departmenl 

J...4-1. KOWlIkae. HigasllioMb sn, Japan. 

of 

OIYC""f!hin,olipid> (OSI..5) pI.y imporunt roles in cel1-cell and cell_maui~ 

Imer.nionl which conlrol growlll anddiffen'tlli.,ion in invtrt.tnles., well u 

mammals. In • teria 0( WlIClUnII .. udicI on GS1..5 of marine invtrtebnlt:s. !I(IfI'lC 

novel and UIIIIIiIIaI OS1..5 ~ found f""", .-.em ... .", dtiton and sponge. ToW lipids 

wen: uU'llCled from marine invmebnla and frIctloniIed imo ntuuaI lipids. Jlyeo!ipids 

Ind p/Io5JJIIoIipMIs.Thoglyeo!ipld fnoc:tion _I'urthc:l' f'rIcUonaIcd usinl 

1.. 1'

Bluching of Alkall-Rtnned Vtgl:labl~ Oils with Clay Minerals. Kelto B

510 

by lhe chemiallndu!llry and art. finding inm:ased .W1;c,llIion ...jlll;n.1I industries. 

In the evenl olan occidcr".l ",\elISe of. hazanIowi chemical, the .)'otem II5C$ DINile 

me~aJ dm. I" ~ict In ...hkh din:

tn>lytic lOIu!ion. Adsorbed h,....,."" then I'tXtS with an oW. triglyceridcs to form • 

hydrog~nll1od prodllCt. E.oIperimcnll were carried oul 1,10'C wing. now-Ihrough 

dccuochemicaJ ,UClor openltin& in. holch rccy

512 

numbero(lhe roulllcrions pYC 11SlraiglllliM from 8 10 J4, from misslope Iht alb· 

ne dill;" of.hae countcriUmerionsofless IIwI eight methylene 

groups wen: 100 !imalilo determine. 

'" 

Hard s...f~0tI0~ S. Alif, Olin Chmtkab. 

Session CC Wednesday Morning 

General Analytical II 

Sponsored by tile AOeS Analytical Division 

ccr 

I)ynamk IIn.o;bpam A....I'.tII\' •• I.. UpIcI ChidatK. Prv.h.cQ in NutrtllonaI 

"""h.m. P.W.Part; and R.E. Goim. MeId.lohmon Nlllritional 0""",. 2400 w. 

Lloyd up!\'U'Qy, E.--villt.Indiana-cn2I-OOOI. 

Due 10 the implicalion of voIalilc: lipid .... KlMion compourxb (VLOC) in tb.vt)t 

doIerionuion. the anaIysil or "oWl" oompounds hIlS been or CCln5idenobk: inI..-. 

For dttcnnilWion of VLOC in nlllriliorllli poOOuocI5, " dynamic br:adspKe ownpling 

coupled ...ilh" cryofocllolnlll«hniquc ,""Ii 1I11dicd. VlOC ~ II"IIppOdomo Tenu 

while bo:-ing purp and then backnll~ inlo" gas chromalogn>ph by .henna! delorption. 

l/1jI'o;1ionofbattnlUhed vLOC inlO" o;apillory I;OIwnn aft ... ~tn._ 

lion aI -I SO'C followed by tapid ho1in& ~hcd in" scpamion w;thou! '-d ....... 

mina of pub. The ""leaion of proper C*ric:r gas was .... i~ considemion 10 

a¥Oid .ruf~f_ion duri"llhnmal elermlnlngOoubie Bond PosItions In Mblures

CCO 

MoIfC'lllar Mudding of Monoadd Trlgl)'«ridft by MO~lIlar Mto:hanlcs. Christine 

Cui",. D.P. V=aulc",11 and F. Dumnl. Facult6: Uni"" .. itaires NO de la Pai~, 

Department "r~mi.try. Labonl1o;",de Chimie Mol StnlClUralc,61 rue de BN"" 

cUes b-5000 Nomur. Belgium. and R. Brasseur. Univcrsiti libre de Bru~clle5. 

The.wdy ofllw: .wuctural pmpertie. oru;&I~erides is limited by the dilflcul_ 

ties in obtain;,,!! monocrystals .suiuoblefor X-ray difrl'8Olian..F~ this 

method can ooly be applied lO.... bIe polymorphic forms. Complemenwy 

leChniqtx:s li.Iees for lhese chromulogroph!Clic resonance (NMR) sptttroscopy hoo many advantages in qualllulive and 

qUII01ilalive anal)''';. Dr phospholipids. ~.g.•high dispc~iDll. high dynamics. identkal 

and ideal peakWpe fDt 1111phDspbDlipids and lID respon .... fllClDr"S.11can be used 

eyenin mi~lun:s Wilhoul addiliona1sepMl1ion, and i. i. indepc.wnl oflhe Dripn of 

the ~ipidl. The mo:thDd has been ...Jic instrument 

uscd-tbe Oxidogniph-emplDYs a transducer 10 provide cOOlinuoos mCII""remCni 

of the sp-cd 11.1 which the pressure of Dx)'gcn in I closed vessel dccrellSe_. Thi, pa.pcr 

presents a new melhod which is superi.,.. 10 oIb...- O

514 

(QIlWnins TaBQ. Cheminl iOOm of IlIIltidilY indOOed (~fllll' Kid. ptrttnlllg~ 

of poIlIr C1In'Iponmis IIIIl diclCCln.. canww. On ,... ba§io; of ~ rroeaswanems. .he 

high oleic oil e..hibiu~d stabiliTY Iha1 _ comparable 10 thellyclmzmMedQiI conwnin,_io_ 

.... .sm-yevoJll'Iloon ofprndurtli (riro in the OIls indialcd a pd. 

etmCc rOlf producu fried in the IIi,,, okIC ... nf\owa" oil. 

0", 

F.rrKb or Frylnll 011 Compu!

Chan15Taklngl"btt in tilt ~·.U1 Acid ProrI!.~R8() Soy.bran 011 l>uri"ll 

Oftp )'r) Inc. A.K. VasWttha...,;l V.K. Ty.si. twroun Butler TCchnoIo&icaI h'Sli- 

Ulle."BTl 01_'5 BunpJow. 7.la>.man Bqh. Nawdlg .. j. Kanpur-2.lndia.. 

Thi~ popel' f~ 00 the ~ ofpolyunsawrlled (auy ""ids (PUFA) In frying 

oil5 during d«p.fal f'Yin&. -Ill!. Iludy -. tho",r""" undenaken 10 aS1IeSSthe IOSIClI 

of PUFA which ad~CMly .ffecllhe nuuilionaJ quality ,,(frying mtdi .. ~ f"lty 

acid composition of RElOjO)'bean oil. anlio~idan(..,.,.,taininl! ooybcan oil lAd v..... • 

pui Wtf'e ~udicd under diffa=llCtS of fryinl conditions. The potaJo chips wa ,"'hile .ho: losses of dioenoic Kids amoumtd 10 about 60'1>, Tho methyl csten; of 

oil SIIIIlpia wen mo JUbjeaed Lo urea hxtionaIiun far dcmminin& tho dt.- of 

poI~ .....il ..." found IlYIIhc _ of I'IOII.UfCI...tdun.fonning atcrs 

wen: proportoonallO !he:a- of !hi: ...... -..1 fany ocidi. The acklilion of rom· 

manly IliCd antmidanu (BHAJTBHQ; in RBDlO)'beanoil pmred indl"cai..., in 

,~I ..cin. the lof.&esofPUFA In fryin, ~ions. 

Session FF Wednesday Morning 

Marine Fatty Acids, Lipids and Oils: 

Plankton Lipids and Origins 

Prokaryotic Soorre 01'(1'1·3) PoIYllnsalu.. l~ Fa", Adds;n Aquatic f""" 

WflIis. R. Jamrs Hmdenon ond JIIhn R. SarzmI. !\I.E-R.C. Unit of Aqualic: 

BiochcmiKry. Dep;. BioIot:iI:aI II. MoIccuI;u- Scimtu. U";vm;,), of St,rI;n._ Stir- 

.inl fK9 41..A. ScoIIand, UnitW Kinpom. 

It i.~I)' auumed .h'lIphlXMynthclic alp an: the primary ~ of the 

(....3) poiyUMalUnlled fal1y ICIds (PUfA) ""hid! ctw.ctcrile the lipid!i of eukaryufic 

orpni5lll' in marine rood wcbli;. Prokuyotes in geneml an: coos~..tlcril have b"ftII mil:mall"" 

from the d_ Prasinoph)'

516 

PIIytopIanitlOa ".11, Adds III ReIatloIIlo Zoopluluon 1111_ MariM PoIIIr 

FI*! Wd>. 0erIwr6 KMIner, Alf.....WeJmer InsIituU: for PoI.-...t Marine 

ReIeMdI. Am Handelshafm 12. D-2iSO BmnemoYti\. OemIany. 

The r.tIy Kids from marine ph)'lopl-./uon Jip;ds _ panwly lnIlSferred 

unchanged into !he IIeJIt I"""P of !he food web, !he wopIanklon, or used .. I91'1.).f.ny 8cid 

compositions wen tktennincd by atpillafy pi dvon\aIoatIPh)' (GC) -'ysi. of 

mctbyl esIer deriYuiVC$ and by OC_ spectromr:uy anaIyJiJ; of whm: Ihcy plio)' .. imporllN ..... both ~ and pn:y. Their 

impormnoo to maamoopIanbDn nuaition and infI_ (In lipid dUaitMion wen: 

CJtImincd !lsi", I oonuoIled feedi"llllldy with Plt~_ttQsp ... common marine 

scuticociliatc which fcrdtt on blctC1'iL TItt: cili.te WII initially ralled "" • miAed 

asscmbllF 0( bttctffil for several senerations. separated from its diet and u:!ed as 

the onIe food JOUI'Ct! 10 die calanoid """"pod "'ctH1iD IOIUD DAN ....... serond .roup 

of MGlTio _ fed an IlISai diC1 for c:ompIriJOl1. Major lipid "laNa and fatty acids in 

Monio. its e&P and the two diets won thm quanlifJtd by thin-laycrcltromalopa. 

phy-fbmc ioniwion detcaion and ps dtnxnatopapIt)'.ft\IU~. fifty 

acids in the copepodt and Ihcircgp wen sil"iflClnll), different bet.....,.., the IWO 

dietl;. with the rapida~arancc female lipids derived from bacteril in ""'mlll bod)' 

tis>;ucs ",hich were not oem with tho: alpl diet. In addition. 1Ie".,.,.1 ciliate lipidf 

"'en: not present in ndult Acor//a. 001 did appear in e[!lli and nauplii of lI1liJnalJ fed. 

cilille dief. These JltI.It!ts will be uoed to demonstrate the seJe.:t;vc transfer of f~uy 

acids from bKlcn. to higher tmphic JcYei. and ilf; poIcruiai importante to zoopIank. 

ton nutritian.. 

"" 

fltly ...dd Mttlbotlsm and 1M Rellrlon5ltlp Bdwecn l'hylop1anklon.nd 1.00planklon_ 

Manin arx'lC!. Instilute for Baltic Sea Rc:sc_h. ~ IS, D-O- 

UIO Wamcmllnde, Omnt.n)', 

Zoopianklonic orpnWnI have. hi&h 'ilriabilit)' in their lipid compooition not 

only amooa diffcn:nt species but IlIJo witlUn the ...... JPCCics ~ an. for 

uample. myifOllll1eftUl r;onditions. food and feedin& behJ,yior. Se¥er.oJ IlUdies have 

shown tha (arty acids may SCI'VC .. mat"l

Session GG Wednesday Morning 

Protein and Co-Products-Chemistry, Processing 

Technology and Waste Utilization 

In the Aquaculture Industry 

Sponsored by the AOeS Protein and Co-Products Division 

GG' 

InO_ 01' HelI!'J>rocm;inc oI'So,bn .. Mnt on Its /'i'ulrilional \'allH' 10 

AU.nllr S.I....,... H. ~ KC!oUoand Fmkrick B"t~ .. u.s. f'1sh and Wildlife 

Service. Turn .... I..abomory of Fish Nutrition. J01j Gracioe Rood. Corj\(lf val ... 0( .pptO~imardy 2.100 mg 0( meal ~ mil 0( 

lJypoin and. u...._ val ... of zero). Funher heal;nl did not improve gro ....I/•. RQUI.s 

of .his study suggest .ha. )'OU03 AII",,'k .. 110011require greater heal: trealmelll or 

soy~an onultlwl is gmenJly used commercially. Dye binding and trypsin inhibitor 

HI.Iy. were more ~ve and ....Iiable than lhe u...._ asuy forevaluating lhe 

IIOeqUJlC)' oflleat •.......,....,. of IO)'bean meal. with !he dye binding method probably 

beinl u¢ul ;n ""* laboralOries. 

RfCO'try and Utiliutioa ofCra_fbh Plcmml and Other F~ f'roo:fttiI"I 

8y·Prvdtlds. SamIM'I P. Meyen.. Food S.:ienoe Oepartmenl. I.oum- State Univer. 

si.y. BIIOn RouJe, I.ouliiana 70803. and Hone K. No. Food Scieooe &1 T«hnoIoI)I 

Dept .. H)'OOIIlB Univnsity. Hayang Kyc.a"lbuk 713-703. K0R2. 

Cum:n1 """,ied efforts in crustacean IIIld fish processing inctnSingly an: 

emphasizinl!he m:overy and uliliwion ofby-producls looitionaUy IrUled as 

wasle,leadi"a 10developrr>m' of. vouiely of value-lidded rommodi'ies. Amona 

,helle i.!he caror""';;d Il5I3Xanthin derived from I.ouisillllll cnowfish. oomprisina ,he 

Ia1JCSI cruilkeln f..... ing industry in tM Uniled SUlles. AstuInlhin Ii.~ianificanl 

romponent 0( Slimonjd didS for ill val ...... pilfl'tlllOl' and role in .......... 

metabolic: fWlf;tionl. A vvicly 04 Olher polm.iaJly valuable by.procIucu _ noced in 

projection or •• otal by-prodllcl u,iliwion 00Il«pI. CruSUICeaJI mr:als _ widely IOIi_ 

lIud in ...,..nahlUl:,apodally in ~1Ilf' f..... ;n.... dietary proteinJchilillOllS ingn:· 

dierm. Such meall a1i1O~ ... IIOllIU of lho: biopolymer chi lin mel ilS tklIcctylal' 

ed derivallve chilOSllJ1.El_hen:, allent"," il beina,iven 10m:ovcry of protein 

hydroly."les. IIOlubl. proteins. mmino acid!;.oo a vouicty of se.food f\aV01'll. Cnt!_ 

I_~n l'fOCeHing by·products are belnaconsi

518 

founclry Iypes. chilled iron lOlls. compound caui. nodular iron 10111.oommon roll 

failun: ...... one! f..:ton alfcaing ~ qlLllily ~ the flaked pmduct. 

Hill 

Automaltd Calero! 01 E,,;~1n'$. Ray 811dlt:kw. BKbeIor ConIroIs.Ino;,. 123 N. 

wuhiJt&ton S~. Sabetha. KamIII 6M34. 

A com oil updlina Jl!OCC'SS _1IUIOmIk'd from the I'tttipl of gam from the 

mill Ihrougb dr}'in&. upc:lw., and fillerinJ of the oil. ~ filter is • lDC$h-tyl'" with • 

dilllomKClClll5 eanh JftCOaI. The mass flow !We Dr BCfflI imo !he S)'Slem it n'leUllrtd 

by .... imptlCl teak jIuI prior to the rtnl ~. The dryCQ an: in series, and tl~KCOnd 

df)'ft" uoeo. feed forward .cherne on. dellyed btii.~ting the residence time 

ofthe gcnn in the firs! dryl'f. Each dryercxillempenltUf'\\ is measured. A minimum 

temperature of genn •• defined as a l""""iJSive to ;ntmd~ the germ into the 

upellen. Theupeller n:lies upon won: eneru 10.. i..iu intemal5Urf'1ICCtemperaturn 

10.... lICCt'pllIbie womnlltmpenuun:. Theupelkr feed rail: mUSlbe ,...w.Jly 

ramped 10 • dII::NnbIc opemina nac. Wort. '-I 011 the pmducI is CClllmlIod by lUI()nwically 

l1IIniPUIIrina thc jaws. The inausc in production has been signirlClllt. 

HID 

Preparalioll 01 HIc~ 011 CoAlml Seeds by Elpanden. Mauric:c A. Williams and 

James F. Cnwfanl. Andmon lmenwional Co!p ••6200 Harvard Av"" ee, Cle-\'dand. 

Ohio441OS. 

At thc 81st American Oil Chemi515' Societfl meeting in 199I),!he di~ UII\I· 

• ion of full-fat canola wasdi~. Rul':d canola It 42 .. oil was IJarHfonncd into 

coll

~. Abscrbma: i.ploucd againil oIrlCiaJ AOCS f_ gossypol 

method f....sampla romairting me gndc:z. Food ProIe;n 

R&D Cellier. TUM A&M University. College Slalion. Te.ll 77843-2476. and M.O. 

Nispc:ros and E.A. Baldwin. USDA CilfUs and Subtropical Producu Labonotory.6OO 

Avenlle S.. N.W., Winle.. Haven, Aorida 33811. 

WUeJ ano widely u..ed as food additives and aseclible coaI;n&" applied di=tly. 

in combilWioo willl othtf lipids. or as emuWoru;. In the .:urrem ~h. uses of 

WU emulsions ~ $lUdicd for pn:pamion of edible fillllll and as clouding agmlS in 

bc¥CtaB= Wax in ......Itt (wfw) emulsions wm: obfained with panicles similar in 

size and disll;bution to oil·jn_wmercmulsions but had benerlUbility (~ 

and creaming) pmpcnics. Selected liuilS and ngttables wen! o;oataI with w/w emulsions. 

and changes in moisull''' loss. ripo:nr:

520 

Imt)'. A number of pruceMlna >leI'" in !he manufaclunnl of niekel h~ 

cataly:ll w=! b~1Id 10impo:wenw1l! cfrom and IeChniqua of _i5Iicalllnalyois. 

ConIinu; oIimpmvemenl and anal~ have ridded drtnonJuabk ~ase:'I 

in pn:odIacI pnfOflllallt'C and consisu,ncy .. ~ml II)' ltlIditional u.ristial 

..aIym 1001.. DIll. ~ prcsmled ....1I~h show improvancnl in CIWII~ activity and 

I:WdHo-balcII ronsislency as • !QUI! of inrn:asing ~lil)' indices for 

..,1ec1cd puamcl."... and drcrusing process parvneler variabilily. Oau ~ in lile 

form of processica ••. The obse""cd inillal 

capolcilY of lile ~nI in I.borauwy balcll binclUnl operimmlS was '"II'ivalm! 

or slightly beller lhan !1Ia!of pranium K"id .,,-rivaled bieaclling earth. 8ctler pnfor. 

mana: was observed in ~inuou. plCkcd-bcd bbdlinl JY"crn. The!lfk'n! adSIJr· 

bo:nI is reJCfOenlcd by IhcnnailrUlmmI .. ]OOO'Fin air or nilJ"tlCm ~ 

Key 10~ful R'pc'lIcd u.., of !be .JsorbmI is a supply of ronwninam·f= oil 10 

the bIeaehi ... operation. When contaminants sudlu pho5phoIipids. KGp5 and II"¥e 

mtWl wm' I't'mO\'td b)o si]~a .JsorbmI,!he IhermaIly re~ silica/alumina 

~ was ~ully IIied for six plus cyclc:l ...ilhoul pnfClf1'nal"lClepenally. 

Session JJ Wednesday Afternoon 

Physical Properties of Surfactant 

Systems 

Sponsored by 'he AOCS Surfactants and Detergents Division 

JJI 

Binding b .. t~rltlS 01' Anlook SurfKlanl.l' with C.tkonk Polymers In Wiler 

,,'1111Sped&l AUtnUon to I~ 

Alloi and Hiromichi 'libIwhi. 

Halr-Condhlonlng Sh.m~ 

KAO Insli,ule for Fundmlml.1 

KIOI"UTsujii, Kenji 

Rcwarctl, 2606. 

Akabanc,l~hikaim.,hi. Hlp. Tachigi 32].)4, Japan. 

Binding i10lhnms ohnlonic suri"actaniS with c:alionic polymers in "'1l1er 1Ia"" 

bten Sludicd in COMeC1Ion wilh lhe hait-condilioning effect of sllampoos. A Iypical 

OOthenn. t.,..lOdium dodccyl ",trite- (50s) 10carlonic cclluk:>se, shows , ........ rp 

binding ",i.h increllJln, ~onco:nl,..!ioro of I"" .gem. The firs! o!fCUlc rOIIIe. r... alonl i..long uil in !he cylin. 

der. but.1Ie Matioro ofCI(,DP·AI molecule i. n:Mricu.:1 by!he: 5Uon1 CI6DP and 

AI ion bondinl- 

An botropk.lly Com~lblt ......ngmnlr Trough and lis Ulllizatlon, M. Mal. 

sumoro and Y. Tsujii. Inllilule for Chemic" Racarrll. Kyoco UniveD"y. Up. Kyolo 

611. K. Nakamura. Sanyuh En,inccrinILJd.. 3-1-1 Ninomiya Hlp$hi, Akita ...... 

Tokyo 197. and T. Y..tUmDIO. Nippon BelllJd~ 6-1-5 Ebiyo!. Fukushim.o·ku. o.u:.. 

5S3, Japan. 

A ne lMpnuir ...... Jh ...hich can iJotropially oamlft$S I spud ..........ya 

oro waIer dcvclopl'ld. The lan&muir ...... gh is disc sNpcd from 1 lOp view. and is 

composed of OIlIer and inner troup ICpBIICd II)' 1 OOII(lft1trica1lycin:uIar.ohapod 

wall. aIthouth !hole hI~" bem ronnecwd 10ad! odICT through a channel in !heir 

boal;lfm. Vefy !hin, bMMIo'ohIpcd dilflhno&rns willi teflon c:oarlnp Iuo~e been put 

inside !he ........... can """",,,,1rif:aI!y C(W« !he ~ surf.,. 01!he i_u-ough b)o 

!heir projonion. thus cnabli"l a monobya in !he inner btlUgII. 10 rompraa ilOCmpi. 

cally. The ouler ...... gh rornpmsatcIa liule chanse of _ volume. in !he inner 

btlUgII. with !he monolayer eonIlftUion. The buoyancy dfCCl on !he ~mml 01 

surf.,. JftS>Un: (b)o a Wilhelmy "",Ihod) ...... lIIus kepI: .. omaU U pos6ible. SurflCe 

pra$Ul"C-aru i50lhcnni ofllr1lChidic lCid (pH S.H. ]9.!i"C) were very reprodll

stale at monoIa)'U'S '" a surface ptHS"'" 24-25 mN/m. Tk lqII'Oducible i5Othenn& 

also Were obuined for lipids and polymeB. A very high suof..:e pressure, web ,.. 72 

mN/m. "'U eaoily mea5WllbIe. 1'hese ~. were in good agreement wilh lho5e 

fouod in Ibo liler:alure. 

JJ6 

MbuurtS of Puked Akohol EIboIylatH-l'«l'ormana: and Surface CMmlstry. 

Dewey L Smith and Daniel T. Plwnmel'. Visu Cbemic:rJ Co., P.O. Box 200]33. 

Ausc;n, ToMS 1872(l.{l135. 

Commercial akohoI o:tI1oItylalesCO

522 

number. Amon,.hc UPLC mobile ~ CYlIhwcd, fICeIonilrile'8Ccwne-nlC1hyl 

1m. blalyl clhe'.lItt'on'lrik..fthanol·bu.....: and K

,·h. ~lUICnoic acid/lWllbulylhydroqu;"""", and roond thaI they were inelfective 

in check;"11 the deteriOl7llioo of frying tcmpenilu!U. 

lU 

Shldi ... on Ihf Standardilatloll of ~'lIt Stability Test for ."ol-}'ryll11: Stability. 

Yukio Al"lI.ki. Ocnc",1 Testing ReseR",h lnS1itute of Japan Oil.1Uff Inspectors' Corporation. 

].J0-4, Milcagel$ukamachi, HigllSbinada·ku, Kobe 658. Japan, Kazuo 

HorikaWII, Osaka M""klpaJ Technical Rosean:h Institute. Osaka. Japan. Shigco 

IWIIIDOIO. Nippon Oil and falS Co. LId .. Nipan. Japan. Tulomu Kawakim~, Miyoshi 

Oil and Fal Co. Ltd., lapan. Masahim Furubwa. Fuli Oil Co .• Lid .• Tokushichi 

FWUlji. Nioshin Oil Mill.., LId.., Japan, Himllum; Zenda, Honen CorpornIMln. Thk.asa 

Nat.ano, Ueda Oils and Fats Mfg. Co. LId.. YOIJIiaki Hin112. Japan Food Resean:h 

Labonuorics. MichilO Hiroi. ShaWl. SangyoCo. Ltd .• Kyozo MDrimolo. Hiroshima 

~fCCTU .. 1food Technological Re5ean:h Celli ..... Hiroshima. Japoon. and Kcnlaro 

Tsmsumi. Rinoru Oil Mills Co, Ltd .• Japan. 

In a previous paper. the authors (membel's orthe FBI Stability Commiueeoflhe 

Japan Oil Cho:miSU' Society) ~poned on the resulls of fal thennal oxidative !Uabili· 

Iy. Thi. paper pre.em .. the resull!> of coilubOf1!.live ICSUon fu,·frying Sl3bilily. Soybean. 

canol~. palm oils and lard were uamined 01 lW-e using a simulaled deep falfrying 

appanllus. Carbonyl. ani.idine.nd acid values. as woll as polJrCOffi[JOOnds 

incre_d lineurly. Rel.lion offo.minS leodo:ncy under Ihe fUI·frying process and Ih. 

variance of m. carbonyl value or the polar compound. was discussed. II appe.rs th.1 

the: .valwuioo offal-frying swbility WIIS possible by means of the carbonyl value or 

polar compounds using m. simulaled deep fat_frying apparalUS. 

III 

The Anal,SboflM Voblile.nd Non,oIalile Pnxlueu (;f"Ml"1IlflI from Ihe HHI' 

ing of Triolein In a Deep-fal ~'r)·tr. William E. Am. Symon Mahungu. Slevm L 

HlllUeolS w

524 

MM' 

Blodtgr'lllbble Ghlltl1 Plastic and Itil CluIl'1Idenzatlon. MilSoo Yasui. Hyogo 

Pn:fcctural Institute of in;;ves until the 

19SOs. whe!1 they wen: n:p!""ed by cheaper petroleum and natura] gas derivatives. 

There is n:""wed lnterat in using iii»' pr",ein in this application due to poIential surpluscsof 

soy proIein and the inevitability o(petroleum price incn:ases. One of the 

major n:ported disadvantages of iii»' (lOllr in this applicalion is the lock of bond 

stn:ngth and d..m.ility. compared to pe1roleum derivatives such as phenol f""",,ldehyde. 

panicularty when wo:1. To betltf undemand this issue. experiment! wen: carried 

OUt10de_ermine the effect of carbohydrBle level and protein denaturation on the 

IUal.gth and durwbility of the pmtein·wood adhesive bond. Adhesives fonnula!('(\ 

with ~ial JC1'j flour al difl'....,rn protein dispersibili_y index level ... conoentme 

and isolate w...., uocd to make plywood. Results to be discussed i""llMit. compui- 

IIOil absorplion and water 

hydrBlion w...... impmved to voryil1j uten .. in all oftllc soy protein. but opimum 

condition. varied betw ..... rype ofprotein ingn:dient.. Mos.t notably. HTC incn:ucd 

the NSI of soy roncentrau; by about four times (1~!l6"). HTC changed the ao1uhility 

profile of soy c:oncentnItC from a flll profile to one Typical of uOOenatun:d toy 

pmtein. 

Session NN Wednesday Afternoon 

Nutritional and Food Compositional 

Properties of Oilseed Proteins 

Sponsored by the AGeS Protein and Co-Products Division 

NNO 

Effect of Protein .nd Fiber 'rom So}"Jxans on Plasma Lipids In Hum.115. Susan 

M. Poue •• Unlversity of Illinois. 445 Bevier Hall. 90S S. Goodwin Ave .. Division of 

Food:! and NutriTion. Urbana, llIinoiJ. 61801. 

A luge body oflitn:Blwe indic ...es ,Iw both prooein and fiber (mm soybeans 

1o","Cf blood choIwcror concenU1Itiofl5 in humans. M05I uudiel; have comple_ely 

..,b5tltuted JC1'j protein for animal prolein in the diet. Stud~ fmm our laboratOf)' 

have soughl to de'ermine i( lower levels of soy protein intake exert a 

hypocholesterolcrnic .... ponsc and ifconcurrenT ingestion offiher from !IOybeaof 

would augment ,his JeS!>OIIS". We fed 25 mildly hypercholesterolemic men!lO &pro-

!tin (~oJlouJ protnenlS in ..... 1S-f,Iobulin f,...,_ 

,ion. A unique proIein wilh • moIecullr!nUI of abouI JO..lila in the 7S-gJobulin 

fnetim bound ~"""yand frcqumtJy with the pa1lcm •• 19Es. ..."hich .... 

named .. at, Dol JOk. a major a1~ in lOybeans. a..subunitoffk:onalyocinin 

...... al5(I found 10 be allt'!JCllic, while a.' - and II-subunits ...'t'fe 001 rucri~e.Kunitz 

soybean ~in inhibitor WIll ..:an:ely....a>pized by the palicnI·s ....... Scnt.of 

a1moxt all ..... pUlenu w.re...." to lidthe IgEo recognizing the proteins in ..... 

IIS·JIobulin fnetion. TIl,. f.:l suU_ that alyeinin may be: lc$$.ollelEC"ic 10 ..... 

toYbcan-Knsitivc patlen", with alopic dermati,is. 

N~oI 

Anti-Hn~"lensh·t Subsea_ in ••.......... t" So,· ....... Nat!o. Akiko OktImoIo. 

0.,.. of 8rewina and Fmnmwion, Tokyo Uni\lCni,y of Apiaoln=. 1-1-1. Sal...,,- 

....... Seuopya·ku. T,*yo 1$6. J'"I*'. Hiroohi Hanapla.nd Fujilwu Yanqida. 

Tokyo University of Apicultun:, Tokyo. Japan. and YukiKawamUJa. National 

Food R In .. i"'le. Tultuba.. J .... 

525 

In the rqulllOf)' sy~m of blood ~ .... iotcnsin convening enzyme 

(ACE) ClAlyza a ralCludi(J have ~ that thiJ protein can main",;n poIij'iv~ ~ 

balance. mny decfta~.he likelihood of JIIlbl""" diKaK and h.. a posi'ivc effect on 

the K"lUm lipids. SlLKlics wlth humOUtshuve ~n CpanoI wnhi", of dchulk>d. defaned noun lind the ..--. exttaOtion 01 ........ - 

INFORM. Vol. -4. rIO. -4 (April 1993)

526 

able compounds hum ..... denarund. dd'lulled Sftd followed by soI~ utnoClioo 

far oil -C!f)'. Methods for !he ~ion of canoLa pmlein iIoIlles Iuove included 

tho u.lilional alkaline alrW:lion WId Kid or ... ta" uttaClioni followed by _lee- 

!ric. hw Of poIyclcctrol)'le pucipllalion of the pro&f:in. Ow:mial and enzynw~ 

modifocalion of pmtd" 10 improve food USCprupenies has IlIw been explonod. 'The 

odVlIIltaga and Ihonrominp ofpo'ClCUleldC'acribed In the xiemifoc and palen! liter- 

IIIlre for recoa. Philadelphi .. ...,."..yl ..... ia 

19104. 

FII absorption durin, hyin& i. ",wed w lower 5mKW)' IiODI'eS of donuts and 

~ oil eosu. InlCff.,ial lension (1FT) belween f. and WIIer"'"IS I!"IaSUftd as 

INFORM. VOl. 4. no . .:1 (Aprl11993) 


• mum of evaluating frying fUJI and their .binl)' 10 be ~ into donutJ durin& 

hying. The du Nou)' rinS mcd>od .... 1UCdlOobuin 1FTal40'C. The Iff de=ased 

with increas,nl frying rime from 21 mN/m in ~ t.. 10 I mN/m in fa • ~ point 

of diJI:vd, F•• btofption bydonullincreastd with dec:lUlin, 1fT. In order 10deter· 

mine whkh individual chemkal a:mpOnCnll in IUCd fryinl fo 1ft: IUpOfISibk for 

the decnoased 1fT. SUrfllCtlnl1""'"' added 10 the r~ fal. The 1fT "'pidly dccn:ascd 

with III increuc in the concenuuion olll10110dfcalFTdccn:Ned Ibe 1FT., ~U. Fonally.IlWdfryilll flU weee 

IJUled with YViouI.cbarpIiYe. ..... criaI' ...hid! ..., kmwn 10 ranIM: depwlalioll 

try'. 101"'" de BN>.cl~ 8·5000 1'01 ........ Bcl&i 

de II 

. 

In urdu to itn~ the ....,I( life of fin;"""" pro"".,.. (ood crnubir ""' often 

employed in i........ ry; they _ ....... other IhinP. Juooooon 10 modi(y !hi: ayMIIliD' 

lion or fll< and ilLThe aim of thil .....,.,. iI; 10 act • &and knowlcd8" or llruct ..... 

aod th.nmaJ prop:rUes of miAlUrescompr»nl oftri,l)'=i

m.-laidin and trioIrin and ~ cmuloir~ an: 1II"l"),a·tOtOplK'OiKr9(Z).II(~e 

.. the: pro6ucII. Beoideo the oddua of 

a·tocopIKm~yl nodical with methyllinoleatc-pc:nIKyl nodical. methyl a-torophclOl1' 

yepoxyoctadec:aloale illOnleB lOUd melhyl a-toropl!eroKY oo:1ada:adienoale lllOnleB 

......" obtained uncItr the ~ condition. The WIIylle fomwion or aIkoKyl and 

oIkylpc:nlKyl nodical$ from the hydropcro.ide and a·'oc:ophao~yl nodicJJ from atocopherol 

CIU5td the addition n:action. 

001.1 

THrmaI OxJdalion o(nln ~llmso(TrIoIt9,.I&l,.coeroI. TrlIlnoItoy~}'C1!n'I and 

TrlII ....... }I&lyncenUalionI (20-1 S30 ppm) was pqoared. and the 

inftucnte 0( the WIler rooIIcnI an the IIWtollidition WU in.atipiOtl under variows 

prcs>um. 01OIIyJetl (J.8-760 1Off) for 110 It • W"C. and thc:n Ihe n:laDonohip 

bc

528 

Under IlII~rcs ofo~y,.,...1heP"fO~ide val"" (roY)~ with cIccruoQ 

in the Will'" ron.C'JU. InJ*lklllat ... ben .be _III of diuohcd",.II... ckcftQed 

frum 2S01.. 20 ppm (~prnwn: 160 I""')or frum 11010 20 ppm (0:2 ~ 

bdow60810fT).1he o~idali~ cIo:leriol'luion orellc oils _less likely 10""""'. WhrIl 

Ihc D.. idMi~ re8Clion lOOk p.c.: aOOv~304 !Ufl'e oflWu",1 anl;OX;· 

dan .. reh Uniy. 

of Agri

paru of lIUI!ure oaI1 In oddition .wnples of one cull'yat Iwvesled II different loci· 

lions and a numberoftndiliooal and exotic cultlv"", wen: analyzed together Willi 

flaked OW .00 oat bran !'rub and II differem limesof Slora~ The lipids wac 

elIlnICI/;d willi hepune/isopropanol, and !he ~ were sep.ralcd 

on hiaJ!-pmDmlllllOe liquidduum&logn.phy. The ~fi.bIes weee silylated 

and the TMS-elhm of the SIeroI. wcn: analyzed by cspiIlary gas d>romaIognopby. In 

all samples of fully~, inlM:! OllISand in.,. products. a-l of 

toW sterol •. whkh were premt in the oils IIIthe IevclsofO.4-1.6. Sevn5lerol 

peab mnI;n 10 be identified. Vc:ry ;mmanm: kernels and !be embryo fnlClion I\.u 

relatively kss iotalavclWlCroiL 

PN 

Antklxldanl Actlvhy In Oils and li:mubi ..... orMethanolk EXII"IId of Noble 

Oals. Ling Ling Tian and Pamela J. While, Iowa Slale Uni~.rsily, Dept. of Food 

Sci."". &. Human Nul/ilioo. 23]2 Food Scimc:es Bldg .• Ames. Iowa. 

Mcthnnolic e.lmel. from gmlUS IUId hulls of Nobleoal$ were I"-Iled for anlio.;· 

danllClivilY underdifferenl condilions. The cxlr.>l:l from groalS had 1I"'"ICr Mtioxi· 

dam aclivity lhim did thai from hulls as measured by 101101 phenolic conlllni. "" groalt 

~re IUCd OS lhe anliOJlidam SOUR'e.The extnICI was lested in soybean IUId conon· 

seed oils held at 30 and 6O'C in the dark. and., lYC in lighl. During S1""'g". the 

p"roxKk values (PV) of lhe oils were significantly lower with the lllldilioo of the 

ClI.lrICIliwo _the control (lIOadditiVC$). and the PV wen: slightly highenh811 fOf 

oilscoolllining TBHQ. In uddition. the UInICI wos odded to ,,",uWens of the same 

oill and hdd u 3S'C in the light and II6(l'C in the dark. Tho. pV oflhe ,,",ulsions 

COIIIlIinllllthe ClI.lrICIwere $ignifiarnly I..... er than ~rc PV of lbose coolllinini 

TBHQ and thecomrol. 'The U!f*:I also was t!:§led in oils held at ISO'C under frying 

conditions. Hi&h'perl~ siu~dusion chromatography of the healed oils 

showed fewer i~ in molecular wei&hl of lhe oils o:ontoininllthe ClI.tncIJi than 

of the oill conlllinins TBHQ and the «lntrol. Also, the fony acid comJl""ition of the 

he.Led oil, conUlining ClI.trICIhad I higher Ig:2/16:O ",,10 than did tho5e contoininl 

TBHQ and the control. 

PP5 

Errecu of Addlna Varlou$ TocvpMrollblins on the Slabilily of PurI~ Veg. 

.table OIls. K. WIU'no:r.USDA-ARS·NCAUR·FQSR. ISIS N. Unlv~ity. Peori •. 

Illinois. 

The elfccu or variou. Iocopherol "'Iins 00 tile oxidative stability of purirled oils 

~rc studitd by measuring levclsof Jll:roxidcsand JlI'i chromatogmphic volatiles in 

lhe oils after -=celmlttd S1""'ge at 7500 lux at 3O·C. in tile dark al 6O'C and at 2500 

lux II SO·C. Oil5 were first stripped of minor ronstitUC1lls by passing IIle oil. throu&h 

columns OfKlivDted a1uminalcarboo black with diClhyl etller. Pure 

locophcrol~lpIul. heUl. gamma. and delm-in various ... tins wen: added to the 

stripped oils. Completely siripped oils had Ihe lowest slabililY. as eXp«ted. Stripped 

soybelln oil ~Hmplescontuioed 1000 ppm of IQInllocopherols b~t with allllred ... til>:S 

of alpha and deltn. Oil~ with high levels of alpha tocopherol had the bcsI1ight51ub'l· 

ily but were lhe lellS! stable af"'r aging illihedark. Those """pitS wilh high levels 

of delta tocopherol had signiflClllltly gn:aler oxidative "ability than did theo,l with 

hi&h alpha tocopherol level,. Oils containing high levels of be", locopherol (2.50 

ppm) were tompar«l with oil. containing normal levels (10 ppm) while the other 

thnoe toc:opherols wen: kept carntant at 250 ppm. SampltSlhal CDnlllined IIle higll 

beta IOC

530 

;son"'OIIC-fcd groups showed ~U"ltll(! hydropero.idllSC Mllv;!y.~ ,Ita! 05 in Con- 

IInl nus. und signific.ntly lIrt'aler Ihun lho PH·fed 11"'>"1'" fed no il'Ollavones (I' < 

0,0\1). But hydrogen peroxi~ ..,rivlty Wa! ~illnifk.mly (lttn:1I5oOle·~hanilC sySlem ...., give resul15 comp'" 

nlbIe.o """ium tripolyphosplml' IIIId n'tnlolrilol;dit Kid on a ..-dght.for •....,ighl 

.... is. I'bbonue·lactobionale rombiOUlions proved 10 be Ieso: clfecliv •• lhough still 

s'goiroant. ccaJdum liCQuesi""'''. TefJ.Q·TonlC1cr .....w.1... ICm:.howl:d dw 

~ of sodium tripoly~e or zeoIil' by I).~c in IfaditionaJ ... 

ClJIIant"'ted powdoN)'pt formulatlom ron'llning Iyplcal >Odium ptrbor:IlC kvels 

ga.-e compllllblc: or. in _ in~ inlpnoved ~y 2nd .",in rmIOVlI.! f... a 

""'ge of ICSIfabrics. Wtihing I~ hoIv. MI~ shown 111:1.ptrbora!e·lIOttharalc and 

pcrbofaIe-lactobinnalc.;an act ,"ro-builde~ in «oIllc·bulll formulllliom.. D-satthol· 

nue ..... >Ilown LO be a co-J(IIu'e for Mldlum pcrlIonlC leuuhydnotc. considefably 

incrusing OOIbill nile of di~ulion IIIId its fin ... IoOlubili'Y. Washing 1e!;tson "'Ioc, 

OU5liquid formulation;, ~ on ptrbonllC.J.ICCh_e hI,·c s/>o:w;nllull ,hel;ecould 

form the buiJ for IIqI>eOUS liquid dot.....,.$. provided ... i'able .... bilizalion an be 

ocl!ieved. Sna:haratc IIIId lactobiooate are ~..,""n 10 be nonlO~ic. "'" ob!~illnl from 

reoc .....blena,u .. ' iOUren lIIIIl.re lihly to be bMKkpbbk. This ort; has demon· 

51n1!ed ,ha, they hold promise .. cnvi"",.ocnUllly Itteplablc bu;Ido and pnovi

~ dfOCl of c:oncmtnllion. pH ond ionk IUaIgtb on !be viscoclaslicily of high 

molecular '8h1 poIyllCl')'ll!c IIOIUlions""'"' Slodiro. Ablwe _ ~n ~n\11llion 

lhm;hQld, II v;!IC'()!li,y inc:~ wi,h I'QpiOCIloconcentnUion wall $ignirIC&nL. 

'",,",uinlllhc ionic 5lJenglh Molcms in ",h1e the washing perfomUll'lCe of lipase 

"hen u~ .uddilivc in dI:t~. 

Mel"lInt:Strurture 1/,1Fune'''''' In" P,~ud_OIOa$Up..,.. kick BOil. S. Wu. J. 

Doubcmuln. J. Van Bielen. S. Power. O. OnlY. S. Nonon. A. VIII Kim .... nadc. A. 

J.,....gm. E. AIh;!'awa. M. Bo.;Ion. C. RtqIl"dI and AJ. Poulosc-. GO ~~ This lipase till> bcen crystalh>;al. and" 

maIn cham t/Xu., hzi bcen cluc:iobted rrom ~''''y crystallopaphic electron density 

map. llIc ...... n dialfI folding b Slmil loll ... .!IttIl in orhtt 11..- ancI CfU}'tne< 

""IonIJIRJ 10 m. class of enzy~ ·n lISthe

532 

abilit), 10 dtlJnlde appk (..... Golden Delicious) cutin. Released culin """""""n ~ 

analywl by hl&h-pcrl"onnant'e liquid dlJ'("".lOtJ'&Phr and ...... lpOI'llive ligh1·Kllter· 

ina deteaion. '!'he pH Opfimum for CIIIirwc _iv;l)' of lipilKS was 8.0 and .o:I;vily 

was ~ by addilion of~. Under .imal conditions. lipases wen: able 

10 hydn>l~ up 10;1only'''' of !he: poI~ .. ~ 10 2Q'I, for !he: cutinuot from 

FMMIri ..... .., 

TriKyl&fyttnllU..- Cootal)'Hd"Jd ....l"bofN.lurai Galadolipilk. N.U. 0Iston. 

KlfI!ohamns Lip;dTeknik AB. P.O. Box 1.5200, 104 M Siockholm. Sweden. 

GaL..:tolipids an: imporum s!n>C'luraJcomponmn. in IUlu",1 planl 

bKNncml:>r1lMl.Eump~ of me.c an: galKIOIIykliKylllyceml (MGOO) and 

digat.to

,S> 

Cholesterol and ."Illy Add Compg!llilon of Upids in Ra ... and Cook.,.. Ground 

Meats: Bffi. BHfalo lind Turk~y. YingZheng(H~ Stuee. Dept. uffood Science and Technology. P.O. Bo~ 

1071. Knoxville. Tennessee 37901. 

New ground melI15, ,uch •• mfalo (OflL) lind tuJ\;ey (On. ""' being mar keied 

biIsed on their lower levels ofchole~terol (CHOL) Dr f"' Ihan beef (OB): however. 

few repon. ui'l 00 tile dim:r comparison of the lipid composition Or the CHOt in 

the three meal. (M). Therefore. CHOt, molsture corUenl.lotol Hpid level (Tt) and 

the fnlly add compositi(>l1 (FA) "r ....unu.l (NL) and phospholipids (PL) wen: determined 

in Ill... and cooked GB. GOL.oo QT. Five replication. We,..,completed: _"'p 

consisted Df the tl=e types of M and IWO In'~. IlIW and cooked CHOL identi· 

ty from hif,h-perfQrlTlllAC'e liquid chromarogmphy IIIUIly.is _confirmed by IIWI 

spccIromctry. Cooking dcc:rttscd moiJ.Iun. level (P < 0.05) but had no dr~ on TL 

and CHOt levds (dry basis). GThad 14O'J, [beCHOL (p e; 0.05) bulless Tl.1han 

GB MGUL GB and GOL had similB1 levels ofCHOL and 1L 10 NL GTIuod Io....er 

levels (I' e O.OS) of p"lmil~ (16;0). Jleuic (18:0) and oleic (18: I) acid. bul higher 

levels of linoleic (18:2). linolenic (18:3) and ouw:hidorlk (20;4) acid. than GB or 

GBL In PL. GT had higl>es! Ievel5 of 16:0. 18:0. 20:4 and teuacosanoic (24:0) acloh 

and Iowcstlevels of 18:3 and eicosanienolc (20;3) acids. The FA ofG8L WI.$clOSCT 

I!> that ofGa lhan GT. The CHOL leve! found in raw GaL (80 mg{IOO g) Wl.$ 3.4 

limes g"'Rler thlUllbe CHOL level reponed on ilS label (29.2 mgllOO g meat. W1:l). 

and lhe high lC\'el of CHOL in raW lurkey mcal (114 mgllOO II M. WB) rna~e. il< 

substilUlion forGB in wdicl qucs!ionmble. 

Attumulation and Metabolism Illl.'iOmerk .·.uy Acids of Partially 

H,·drogenated Canola OIl In Rats. W.M.N. Rlllnay.u. Zbero·Yu Chenand 0.1'l for !ICBSOOIIlv&riationin TC. 14 HCPM women who wm! 

oJreacly following a low-fo.! diet (22,*,) were asked IOconlinue their LF die!. Monthly 

rounseling and edllCalional sessions were held. Only 12 of 1!Jsubjcas faithfully 

o.dhered 10 !he LF diet durinS!he enll,., t.ludy. n.cn, ....en: no .ignir..,.,,1 changes In 

,he lipid ~'ers in lhe conlrol LF group. bu. TC dKr=sed from 264!0 238 

mgldL (p .. 0.02. n _ (2) in lhe LF/MUF sroup. TriSlycerides we", unchan.ed (1S1 

533 

vs. 1S6). and IIwm _. sligh!. but not .ignirlt2lll, ~ in HDL-C(50 '·s. 46 

mgldl). Inchwm of !he high-.oleic peanul in lbe die! ..... very wwyins 10 !hese 

subjects. and IlM:yindiClled lhat lhe educational marerials lind demons!BIions had 

been &'"ta!ly responsible ror lheir abili!y 10 comply with !he diel. II i5 "igh choles. 

rerol die!. Three ",bbits received 4 mL of E. CO{!r"QS1QfU>ligtlftS suspension (_2 ~ 107 

cells/mI.) daily PUQS (or 10 d: IhIU romrol rabbits received Ihe same do""ge of 

boiled bac1eriuJ su.pension. Plosma choleslerol """""mralion oflmlled rabbils 

(lg9.4 mgldL) "'lIS signirtCanUy lower (P . respectively. 

Purifil:ation of DHA was examined after CIhanoIysi. of orbitoJ fo.!oil obtained (rom 

a luna fish and resulted in 95-'.l9'1> purilY ofDHA..,lhyl CSlcr by. ne ....melhod u5ing 

Ag ion II quile. low m51. We """,,rved tha! Eol-I. a human eminophilic ~n line. 

acquired plalelel-.aival;ng foclor (PAF) produc,ion ,"hen In:itled ",il~ )'-IFN. The 

JUpplement31ion o(highly purified DHA!O the culture resulted in significam 

decrease ofPAF produc!ion in A23187-s!imulaled Eol-i. Fauy acid analyses of 

phospholipid Ilnd!be study of A23181·lnduced fally acid ",I......., ,uues! thai pIIotpholipa.

534 

NIDDM. HTN and CHD. Thi. may mean !hat spec,r", Ihonpeutll; Slnlle,ir:scan be 

dcvelopcd with BFA. in thaoc Il=e conditions. 

Session TT Thursday Morning 

Marine Fatty Acids, lipids and Oils II: 

Trophic Transfers and End Products 

()dadeno~nlMnok: Add: It. 810marktr rorToll~ "brlM Phyloplanklon? 

Jeanne D. JOKph. NOAA,INMFSJQwieston UbonIiary. P.O. &u 12(i07. 

CbarIeoIon, South Carol,... m22-2607. 

Marine bioIo.ins and IwmfuJ aipi' ~I' &ignirteaOland c~panding IbreM 

10 human hIIal\b and fisheries 1hn>II&hw1 the world. AI the praenlli ..... the ~ 

oflOr.ic ph)'lOplu>kl(m in .... _column is moni1lnd by !he AsIociIIion ofOffi· 

cia! AnalyUcal Owmoo _ bioQay whicb is nonopccirw;, n:lalively inseniilivc 

.nd ~y 

(1':SaIl) 

URKUplabIe in mM)' COUIIIrieL In 1975.oo:tadccapent8c 8cid 

101" rlnl rqIOI'Icd in !he lipids of eleo.'m species of cIinoIlqt:lllles.. phyrop1Wron 

1haI_1IOIOrious for their invoIv=>mI in "taI1ide-,...mlJ. Since !ben. 

Ibere ... "" heal opo:ncIic rqxxu of 11:Sin lipids0( OIlIer di~llales as well loS 

in Ihooe 0( _ sp«Hs of ~yte$. abo irnplio;amd in red !ide ~ 

R~dy. 18~, il5C:lf, w .. rq.ortcd II> be an ichthyW of FISh::ries ThclInoIogy, 

Hallfn, NS. Canada 8)J 2X4. 

~ lipid c:ompooition of marine orpoisrns can ptOYide val ..... ph)'Siolo&ieal 

and eooIosieaI infonnalion. hI Ibi, Jludy ...., u...ined the inc:orpanUon of diebrJ 

fany Eicb and SICf'OlI and determined !he anawmieaI disuibution and .sea5OOII YlIriarions 

of IipMIs in Ille Nortb Atlaru~ _lIop P/Qcop«It!~ _,..Il/UlkMs. I~ 

trimethyl~ add. derived f...... phylopllnk1On chlorophylls, ... pftSaI1 

only in the triacylJlyccrol fr'llClion of the dipve &land. indicMln&' catabol~ route 

for the inSOUled planl pll'I'C'''"- PoI..-lipid fany odds ohlgal ori,in found in the 

""alIOf>'. IIM:h .. 16:~1. 18:4fIl3. 2O:Sw3 and 22:6coJ. reflecled seasonal 

fluctualiom In lhe wile, tcmpc:rature. Changes in !hi: level, of these Ilgal .ign.oture 

flny.cids in the lIlein were. found 10 be 

the _ .bundanl. Ilowe_.all of these moIecular~"" _ ... the:_1c:so 

than B

.. lric:iroupentaeooin and tridoroube~aenoin ....e'" !mOe wnounl$, There was • 

d«reue In TO mol«ular .pedel th'l coo.l~t of al leas! Iwo w3 fauy add moieties 

...hen bil"'~cd tuN Ofbi,al fit was na.ndomiud. while. on !he other haod .... tIm bonilOorbi\Al 

f.t and squid oil _.., nndomiud, thoy j""rased.. The incraJe in TO 

moItcul..-lptCies !hat oonsiJl 01., leas! two 103 fany ocid moieties .... the oil 

more AtKeptibie IIpinst OIidMion. 

Tbt ."1", Add COIIIpoiIOon otVar\owI Lipid C ....... ill s...n-al ~ ot Fbb 

CallJhlln IC'elandk Wlltt'S. G.O. HanJdsaon. B. Kristinsson and S. Gudbjarna-.. 

Scien.:e lnS1itule. Un;v. O(I~land, Dunhap l, 107 Reykjavik, IttlAnd. 

The IIbjc!ctivc of till' ..rudy w .. 10 in~iplc the lipid compo5ilion ohevenol 

common JflCCies of !ish caught anI

536 

UU' 

IndlUtrbol I.ubrkants and Addilh-.,. ~rh'e

lion IIf .. tunlled and unsaturated lool-chaln fauy odds 01110v"';ous ~ydrales. 

Sll'IIC1ural fe.rures of the$c maleriaJl hAve been clumlocleri.ed by solid·suue and solution 

nuclear magnelK- ~ (carboo and prowcd IMI the Induaion lime for aysulUwion ...... 

markedly dependenl 00 lhe aySl.lJiuuion lempel'llure lind ~ to opproooch .... 

uympl(llk value ~ .boo.1I 4O·C. BollI oillypes; """" .JJO oI:o!;rned to dwtp: fmm 0: 

10 (I.fype ofpolymorphic form II iboul )O'C from X· ... y IrIIlysa This was 

supported byopIit:aI miaotoope observations "..hm; lhe aysIals III U'C wt'fe 

oboerved to exhibit small aytaI ~i~ with ...... 1 necdle+like owgm ..w in • . f_ fan~"",id 62.6\12'\110(palmilic add). pmlaide vol...: ).499. iodine .... ue 

49.s90. oaponlf'lCalion valuc IRJ.I and umaponifiablemallerO.!lI. HcalceUenl 

cpimeririnl.blilly in MI\ICOIIlliOlulion could be developed. Forexampk, Ni cnm· 

plcx con .. lnlna N.N.fIi .... lhyl-N· ..ahylr:1hlencdlominc (1.1,2' --en) showed excellenl 

epirnerivng.bili,y in lIle1hano1. buIll' WI\CI' it showed no ability.o epirnerizc 

aldose. On the other hand, wlllm N.N •.fIllIle1hyl·N· ·hexldocyl-dhylencdiatllinc 

(1.1.16·--en) was ute.i. reached i.1Iw !be difference of 

the epirneri;tin,atMlilil!J amon, lhe NI(II).fti:omirw oomplexes _ attribulCd 10 their 

agrcgallllJ propcI1y In the waler boscd on lhe amphiphllic SInJCtIlrc.. 

vvt 

Inl_lng Ch.nac In 1M field ort:diblt> OIl .nd Fat ProdItCts. N. Mawai. Nlljima 

GUuen Women'. Jr. CoIkJC. 

vva 

The Pu!en.btl U~ or Ken.rOlI .'01' Uuman Consumpllon. Ali I. Mohamed. H.L. 

BIlardW1li. A.A. HamamallOd M. R ... S.ppo.. \Ii'llnl. SIa[~ UniVft'!lity. Agricultunol 

R.........ctI SllIion. Bo" 92.59. i'o:1cn;bu'l, Vi,.;nl. 2J806. 

INFORM, Vol. 4, no. 4 (April 1993)

538 

~ is. arow'"1 inl~ m1M uliliwionofkmaf(HibiKlU.--billlU L) 

as I SO\Ime of pIIlpwood for I"'PI" ..... ""~I indu.uics. Kenaf......ts COIII";n 

..-e man 20'1. oil. The !ftgcd from 

I~ for Gualcmlli. 43 10 I.B3... for looi. voritly. The oil of7B·18RS·IO _char· 

acterized by ,,10111conct'nllllltorl of pI..,1 51m>1. (O~); "'hen:as EVetJ\1a!b 71 

had tho: hiRhot (I.IS"'). Tho: ll-~ilOSlm>l (63.77-80.97"'1. Cllmpslerol 

(3.02-13.15'10).00 Migm.>I~mI (4.07-'M7"') 111= 1'Il'",,1tn1 among !Wed gmo- 

Iypes. Palmi.k (17.64-20.~"'). olde (23.62-3 1.07'1» BOdlinoleic (39.79-47.78'lt1 

.. -ere.he moM abundant fauy lICids; 1IIhm:1lSp;:Ilmitolcic (1.21-2.14"').linolenic 

(0.43-1.11'1» BOd51caric (1.9S-l.8~"') .. '~re praml in minor concentnItions. Medi· 

um lind highcf ellllin fal1y lICids (Clz-C14 rmd C2frC24)"'- kU and 51or· 

........ tonIroIlcd OOfldiliQns provWd ~pks "'hiclllO'!n IWtd for color. f_ 

fall)' Kid. prrolide and ..,,,idinc level .. ond dime and tri ....... fomwiOll. AliI""'" 

o.howtd ltw the h,&bet Idsortoem producIs pvc miueed or similar dc1erioo1!ion of 

the ~Of\'d or "3'Id oils. 8y lJlkilllldvaNa&eof rtdu=lllddirion level .. Iho neW 

product an produce oil. with enhanced .ubil;l)' blU will rcduccCOiU_iated 

with oillos!l and ~.dt ......mcd oil i. If"ClIIed.. ·;,h IIIICK1 IhIr is subKquenrly panially ntvIl"llized; 

or c.... he combined "',m I1IIOlhcr ... r",'''1 ""fC. auch lOS ,n; (i) drydqumming ~ 

drlumminl is combined .. "to bleaching or (Ii) alkali relining .. rw:.... dtgumminl i. 

oomboned irh chemical ntUlra1iulion oflhe oil. Finally.lhm: is. ",,",cl proccs.. in 

...hich the shin. _tcr Is /'dIICI '!""Iily.ln ClIlUlic rdininlrhe 

prinwy and lllal" ..'til! fCIIlriful" Sleps and associllled by·~ ...a>le we_ 

an be dim"",u,d. Lrrbonuory MPR ~llO rorooybcan. canola and pIIlm .. -ere 1'Il'. 

_,ed pn!viou~ly. Addilionailibonuory woot ..... doni: to ~ppon nco-up. Acid 

.re~'lMnl;~ required ... hen oonhydnl1able phospholipid< arc prI'5e11tin lhe reedstock. 

SoIp-ronll;ninl oil mu§! f00llIin Ies!;!han 0.2'1> moialure hef~$;lit:o IIhoobcnr i~ 

added IQnlMlre COI11p1ClC soap removal. MI'R IIu been dtmonsrnl1ed al ""vCftlI 

rcfincri".. Tl>esc include: physical refincriClllreating onode p:tlm BOdsoyhean mil 

and a cluMic ~t1ncry lrelllingdegurnmed ooyhean oil. 

wws 

Mellloos ro, IfHlRyslnK .:SloUde Yields In a Balch lI.elK:I'" Dnd Their l>tromposlll"n 

I'allc.n. Sclim M. ErhIll1, Terry A. Isbell and Ruben Klei",,,,,. USDA.ARS. 

Nalion~1 Center for Agriculluf'll Urilizalion Resean:h. 1815 Nonh Univcr.;i!y S'lffl, 

f't,oril. IIlinoi~ 61 604. 

ESlolidcs .• hydroJ.y (ully lICid in ....hich rhI: hydroxyl JfOUP i~ CSleriilCd lIIilh 

another fany lICid. ""ve pot01l1;.' applicarioM in arcll5 511ChI15lubriclLllU. f_'~ 

and .wrf"IICI ...... 1lIcy ..... formed directly from ~f'lled farly Kids willl_er 

and dly CIIal~lI under h;a/I lempcmun: lind pR'UUre. Yleidsof 12'0 14'" an he 

increased 10 :zoo. w.,uJh .he manlpuLorion of the equilibrium by rcmovaI ol water III 

oppmpria1C lima ESIoIidt: yields can also toe inr:reased approJIimlW(ly 2'* ...hen day 

catal)'*lJ .... ""ared ...illl suitable delefJCftU. E.uoIideo hive bcm found 10 be quile 

llI&bIc. They "',..., leMed under hip 1Cn1J1CfMU .... modrrarl" fricrion and op011 10 lhe 

IIInIOJphm: ",illl l'1li .i .... i~ docomposirion. 

CUll..,. In 1M PIIosphollpids In i'nnul5 S"bjmed 10 I'o5tha.,.~ TtmPft"llun: 

s.rta J ..... SillJlc10n and L.F. Stikeka&hcr. United StarO$ ~ of Alricul· 

.un:. Aplculrural Rcocan:to Senoice, Nonh CaroIi ... State Univenlly. Bo~7W. 

Raleia/l. Nonh CaroIillll 2769S·7625. 

l'hMpIuoridlc.ad {PAl. phosphIIIidylc1h11no1unine (PE) and phosphIIIidylinor;i. 

101increased in concctllf'11ion ...hen imnwu.., peanullftCb were cuml II. hiJh 1Cn1. 

perature (40'C). ~IUIUI s«d upll5Cd 10 -6"C showed III i~ in all 

phoaflOOIipids utepl for phospIIllidylcholine (PC). Seeds upowd 10 ·16·C ~.

~ PE oor.a'tI ion, an i~ued PA concallratiClllMd PC concenrntion 

..... _ found in litis pII.ene lfONIfl. 

..,...1 .. ohow tt... w__ 

IlII! _ widely uoed....r1Clanl. u.s. ('odd monitoring 

trutmml COOI¥OfU. port .... MOATS 10 • .,..naJly .... idiKd 

form. The balana! il eilh..- mino:: .... u;ocI 00' od.torbed 10 sludge 

OATS beinJ appIiod 10 U.s.IIOiI•• w,,~ dilpOAl. To dc1cmoinc 

...'hkh resullS in 

IlII! r_ of OATS 

in IIOiIotMCI...,.,'¥inl WIIHS. ndKIr-C'Ipin:wnctry upcri_ ....."...condoocI~ wid! 

539 

14c-benzcne ring.labeled Cs OATS. A monllOrin& snody ...... a1sodone IOckteroninc; 

~- 

!he fal~ of OATS """'" • wUle_ ..... _ ........ plant. in IOiIot amended with sludges 

from IlII! pIaN and in !he "'c~jyin&IlrCaIII.. Mlnenliwion ~ L4c labeled OATS 

occurraI wi1Il. vwicty of JOiL.and in pcriphr- round in !he """''';118 JOcUn. 11k 

monitorint RUdy dernoouuaI~ !he mooo:waI 01 DATS in IOib Ind in IlII! ~ 

Oe1tnnl....tlon 01N.-Ionk &orfadanl PWricy byln.row Cal Chronuol ...... 

pily. V. Mart NIIt\'. Dow U.s.A .• 8-410. TuuClpcnlioM. rncpon. Tuas TIS4I. 

andJ_ C. Knoo:II. MoOIkaI and R...... Inc .. 622 Commcn:e A"Ie.. rncpon. Tuas 

TISCL 

11k polarity 0I1'IIIlionk audlOCUlnlleM be aff

540 

Oxidalioo of Jilyl CnolAlcwith jll'fSUlfalC(S20g2'j orKI ~uclioo of a·kCloeslcr 

.. ;tll dilhiooluc (S:z0421 we .. ;nves!;pled ... ing his_ammonium dkllion (~~ IIIi • 

00IIII1... kin. whit" ~ lWOqualmlW)' Immoni"" groupo CQflnoctcd wilh §e'! ba< is ~;n ...artr for. period ofl;""'. lhen allo".1!d 

INFORM. Vol. 4. no. 4 (AprY 1993) 


10 .ir dry. "The n~"''''''nl of Jt.rdneH lhen reve.lllhe ,""Ienl ofmoislure penetnt • 

Ijon in the bar whkh ill ,~nUy 111d.... live orlhe I"",d ofmustli1lQll propenits of 

lhe lUI bu. "Thc bin .tIowin,aluutlncsl or pmen4 durin,lhei. ule by c:anSUn1Cf5. The bar hardne» mea.wrm>em of 1IOf· 

m.1 Jt.rd·milled OJ*IIIC. I.... llucrnt.llnd lyndellOap products ha$ been comilled 

with thrir proocusin, roo.::Iiliono and perfonnance IWlribu,es. 

Session YY Thursday Afternoon 

Enzymology II 

Sponsored by tile AOeS Biotechnology Division 

SlIblllud Upaw'II for lIydrol). RCKlions _.n IndlH(riIII ~ F. Pmus 

CupmII.. Sietu. T. 80uwcr and JMtmMI T.P.1H'*-r. AptlkChhillop;al Re5un:h 

11l$li1~ (ATO-OlO). P.O. Bo.. 17. NUi100AA W.,....m,m. The NeIherIandI.. 

o.,.piIe me;,. f.......'" orloct,~ blw.ymhesii ~ enzymes _ 51ill 

limiled in mo.r 'WIn;'" in mt]IIU

Ulle of I",mobiliud Up.,. ror RK'O"~ ofSlft'Ols and Tocopllerols from "eaflab'" 

Oil Oeodoriu.. OIsIillalfS. Swuh Itomamu.nhi and A.R. McCurdy. DeJ-1mmI 

ot ....pplied MiIIOSido:. CHAPS. and CHAP. 

SO. ACCivity...... slighdy de(1 llftf",. 

~ for uiac:yllllyccrols COOIllininl fauy ..:iW"'. """",obtained. A .uucillrul OOffip:uUon of 

lhe avail.blt: alOmie models (which win inch>flCCi(.. ;. 

,yo" ... Iyt", fu"",1on and ,nlcrf"dalllCflvatlOl1. All the enzymes contsin caral)1'" lri· 

541 

ads and a I1cKible "lid" R'Sponl-iblo: for Ktivalion. although lhe

542 

Ra 0..... Oil-Com.,wllon Ind Cha~nislicL FnuIk T.ru-rer. Ri«Jand 

Foods. Inc., p.o. 8oJ. 927, Srungan. ~ 12160. and Raben J. Nicolosi. 

Univ.mty of M_~t5.I"ow(:Jl. MassxhuseIU01&54. 

Int~ in rice Inn oil,.."...enewed when rhe hypolipidemic propcniesofricc 

bnn. observed In ,,"imal and human trials, .. ...., ((lUnd to be roncrouated: in rhe oil 

componmts. StLKIies on bknded fa" &howePpin8 with .-4(pyridyl l-oxidc)-N-Iut·bulylnitronc (POBN). Lipid rldlcal 

form.tion Will' enh.""ed when the ,row!h medium WI. ""ppl.menled ... ilh a. 

lillie as 5 mM OOcO$llheueooic Kid (DHA. 22:6n_3). Computef simul.tion indi_ 

caled Ihat It leOSI two c.rbon-eenIC"'" POBN spin adducts we'" formed. The 

spectral charKtcrillica ....en: consi.llcnt with lhe pn:Stoce of. pentenyl adduct 

(aN~15.15 0 and .H_2.61 OJ and an ethyl addotC\ (IN .. IS.13 G and all-2.78 OJ. 

These .Ikyl tMliuls probably are formed by jJ-..,ill$ion of.lkoAyl radic.l .. 

Allhough radical adduct rormation was not sp«ir", ror DHA. the amo\IIIt or Spill 

IMldUCtgenerllM wlS 1""ler when the «II. were .upp ....memed durinlll"Dwlh 

wilh n·) ... c:ontpllml with n·6 poIyuniollura,ed rltly .dds. Addilion of .oscorbale 

to the _y medium ,,,,ally ioc",ased the radical signal intensity. Radical ,mcro_ 

tion .... s dependent on the presence of cells. bullhe POBN spin adduct was 

lktected primarily In lhe c11nocellullf fluid. COtTObonilion ofiron·induced lipid 

peroxidalion w'5 achlcved u,inl Ihe thiobarbiluric add n:"IC1ive substa.ncu .~say. 

These findings inditlle thRt lipid rodital formalion i. ~trongly

"'" 

Oxidative S!abllillesotOlls (rom Scltcttd Canola \'Arielles. Willian, E. Neff, 

Timothy L Moun". Wilma M, Rinsch. and H,rook; Kon;~h;, Food Quality and Safely 

Research. NCAUR.ARS-USDA, 181~ N. University Sireel. Peoria. lI!;lIQi. 

61604. 

AmI>- and pho!QOJI;dalivutilbililies, oxidation prod..a IUIdvolalilc formatioo 

and formation rates of the!;e produocts RSpeCIive I"oil. from .elmed

544 

pmdLICH. To cl..,idaIc the ..,.,;tiy;.y of EYP, Y1Irio\l$IoIs 0( ~ prin.R. 

bruk_ dlet«tion owI .... Qmalic shutdown ...1Im • brtI of melt, me!tinS peak muiml. D H (heal of fu5ian) and romptelian 

of moll poim. A pn:limillllf}'collnbonllian between fourtllbonilories idm!ift«llhe 

IlICkof bIuollue ilMdllfdiznlioo R5lhe principal cause of diSl'repancles. The rollnbonl. 

live Siudy which fo11owtd, involvin,eighllabonllories, rouoo !heonlCl of mel! to he 

the more pnclse mtUilre for the within laboratory compari!lOl\ IIIId lileoornpletion of 

mell du... 0 be the more pm:ise hetween labs. However. baseline choice wallSlill!he 

reason for !he OIIlliclll IIIIhe 5WiS!K:alOIIIaly.is.A flJlllJ evalllalion incarponIred • lem· 

perilllllCp in the thermal prurpam which oppratlIlO imPlO"e the IlII1lysu' IocItion of 

!he ~Iine and lhcroby Improve the pncWon of!he dalL 

DOD] 

A N", R~fIc Anal)'1kol'Triao:Yi&lycn'Ols Usin, Allyl MapalYIII Bromide. 

Claws C. Beck.... L.enc \bgnIcn. Annetnet ... Rc.mquisl and Ounhild Hillmer. 

DepartmmI of BiocIIcmiOlry"'" NUlrition, Tedmical UniVft$i.y of ~ OK· 

2800 Lyngby, Ommart. 

"Tho n:po.p..oiflC diwibulion offauy IICidI in trl.qlaJycerob is 01' JIUI impor· 

Wlce III lip;d biochcmillty. bul is difflCUl.lodetermine due III .:yl migruion. ~ 

is an dfocienl method that Iw been in usc f«two yean.. A lipid sample (1-10 mg) i. 

di5lolvtd In diethyl ether and partially deacylaled by !he Gritnanl rugtnlallyl mag. 

nesium bromide. The """hing mi11un: ofJCylglycerol1 i.sepanwI by thin-layer 

chromalography (TLC) wilh boric'JCid impn:1/IAled silicic·gel pi... ", inri. m;~ture 

of chlorufonn/I

DO'" 

Llpld-Sllrface OrpnlUlUon ProbftI by Upase-C.lIIlyud 180 Euhanr. J.M. 

Mudc:rhw .. W.E.. MI)n1JCf)and IU .. Brockman. Univeniry ofMirmcsouo. The 

HOI'TIKI [n$I;IUIe. 801 16th "venue HE.. Austin. MiJ\l1eSOUl55912. 

The exdwlp of 180 bttween H 2 160 and p""labeUt:d domsadicnoic Kid 

(180. J 80-010) caca/yad by euboKylesier lipase and roti~ 1iP"R wq 

inw:~ ,,"m, monolayer and billyer immKe5. In rum. of ISo.I80-0A aJone. 

the oxy..... uchanje ruction protccdcd by • rarUxn, ...qumrial medIanism and 

was limi~ by the IICY.laIion ~ will! ke.JKm of 0.12-0.16 =2pmo!-1s- I. With 

mixed monoIayer$ of 180, lllo·DA and !.paImiloyl-2-okoy1-phosphatidyIdloliM, 

the Ulft'll of lipue-aulyud ~y ..... ~ inaeased abrupdy bet ........ O.sand 

0.6 """'" f..roo;., of DA. Concomitaoll. with IhiI critiaI tramitiCIU ... I dIInge in 

the ~ IIIfdwIism from coupled IOrandorII.. ~ Re5ultswllh unilamel· 

.. bilIIycQ ~ ..... ;1... nkcn 1Of"Iher. the com""';'~ swildlina of 

ItCIivily and runion mechanism IUgati R'gulQ:III by the bunl orpni1.:Mion of 

lipid hud-JIOIlPf in the inI«f_ Sud\. mnsiIion is bc:R IIDd=uIod in the IXInIUI 

01 pcra>UbDn Ihro;wy and _Id play .. imporlld mil: in lipid-mtdialcd sip! uans- 

...... 

0005 

Soh.blllliel of SImple and Mind TriKyl&l}Unlls in Sapertttlkal Fluid COz. 

W.B. Niluoo and J.K. Hudson. NOAA. NMFS, NW FISberie$ Sci. Ctr~UrilUMion 

Rc:xaR:h I)jviJion. 2m Monllake IIQgLevatd &.I, SealtIc. WI. 98112. 

The puI dec:wIo: hu .... ;messed aro"'illl im~ inthe appl~ion of 0..pt1l:ril;. 

tal nuid (SF) Ulrxlion 10the proea!Iing of edible oils_ A complele undenlanding 

of these ~ ..qui .... fundamental dua \0 ctw.:taize the behavior of ")'SIem!l 

tmSisting of ';pick and IUptl'Critlcal n..ids. For eumpk. a rwmbo:r of stuWQ h.lve 

reported iOIubilllla of.implo triacylJlycerols (TAGs) IUCb as Iripalmit)'lglyecroI 

(PPP) and IriokyIJlym'Ol (000). SIICh simp\t: TAGs are no! normally Ihe prcdomi_ 

IWII ~ in nMUnII oil$. which mainlya>nlllia mixed TAGs IUCb III l-pllmit)'ldioIeylJlym'Ol 

(1'00). A model miann. ~ of PPP. 000. 1'00 and PPO 

_ .yn~ cncyraalicaUy. By·produls) wen: =nChemicaIprdermco of ~ 

_ Iynthetiad Ihe tnIIUiomericall JIUft ed!y\ ,,·huyl p-niuopbenypho5pbclnMe 

..hidI_ found 10 be suiubk in Ihe dr:tcnninltlon of li~ inhibition ltindia. 

Kinetic studies cIC. fINDIrfica tiptie ohowcd UI CfIIntiolcJecrlve inhibiDon K2i'Kd 

• ] 1.4 mM-I.-1 for Ihe (.)~ and K2t'KcI- 1.1 mM-I.-I for the(+) CM!'Itlomer. 

1bua. • learic acid (12·HSA) forms. thermon:vCl'Jibie 8"1 ....ith ph)'$ical 

lUMIciotion nttWOl'l.: in orpnic soIvenII. In the prescroce of a small amowu ...- alkali 

melll ion. we h.lve round a fomwion of IIO~I 8"11 II It>w conc:entntion of 12_HS. 

The "",wort 1ItNCIW'e .... cum'nr:d by pIwc diagams, ein:ular dichroi .... (CD). 

'nf!Wed r.pecI/I (IR) and electron microloope (EM). Alkali mt1al soaps were wed 15 

a soutre cIalkali mcIII iom. The uperlmcnu wen: curled Out in the DCJRCIetIlrIIim 

ronae of 1-3""'- 12-HSA for !he system 12-HSAhodium 12·ItSAhoyban oil. The 

optimal molar lIlloofI2-HSA/N.+ with I'1:5]lcct to ,eI fOfmllion wu 100,1. Whi\t: 

the muimum .... ~lensth of CD opa:UUm derived from lhechira!ity or 12_HSA was 

kepi COI\SIInI, lhe mnarbble molarellipticilY [8[ decrease wasohscrvcd with an 

inc~_ in the conccntnuion of N.... IR opu!lI indicated lhallhe inten.ity of 

absorplion bands due 10 the h)'drogm bonded ..)'dto~yl &fOOl" was kept oomtant 

with the addiliorl of NI+, Funhermon:. the fill(ly dilpersed fillets W

JOlUlions. Thr: .... iI;.,., of tmall """"",to of 50s ~ the larJIcst ",lv.aIion 

lime. The ~ pIaccau modu.JllS ...., alrno6I indtpcndcnl 0( the IIIObr friction 0( 

50s. bw inaaocd with JWf_, concen .. ",;':'" Li&IM oanering ~ 

di"~the u~ of; mnicellar rood_ion. II wuttmislr,)" Yokohaml Cily Uni~cnily, 22-2. Solo. KIII\lllaWll-ku.. Yokohama 236. 

Japan. 

Spindle-IYpe IK'm;uilc panicki (-2 j.Un ;n length) dis]lCrsed in oql>eOllS JOlUIIoru 

formed linear chain_like ."lomel1ll .... undor the innucnce of. weak mal""tic field. 

u.observl'd in. mc1allur&ical micl'OliCOfJC.WIlm liIt' ....eaI:. magnelic field ....a. 

remlJ\'l'd by superimposinglll'l Ulcmal magnclic rlCld. the chain-like auJomcralC!l 

showed random. MW::e-like movcmcnl~. 'The """" of 1M chain WU'C bound 10 form • 

cloKd loop ,,"Iomenuc if lhe ends II9IIf1*'htd in .he rmgc of ;ulralOlion. Thr: 1BIUilion 

from • linear chain-like ,,"1ome .. 1. 10 I eJr.cd loop .gglomcraIc could be 

uplaincd in termS of inlenction encru of the clcclric:aJ double Loyor and lhe m&Jfink 

intmlCtion "::!'!"em hemalile pulicla multing in a ~ial muimum MId • 

KCOIIdory lIIinimum. The cakulaicd potcnrial ...... imum .. hich KU .. I barrier r... 

the famwion of the loop ~ .. the numbtr of the IRf1X:Ics in the dIain 

incft:u.ed u. the dcpoh 0( the 6«OIIdary minimura increas;ed, which agJftd ....;m 

-....~"'- 

1)1)01;& 

AMoI'pIion ISOIMnns fOf Sesame 011 In I COfI«ftll'1lltd Mlscclia S)'Sttm. JorJe 

F. Tom-Vnqllel. FacullAd de: C"'rKiuQufmieu-CIEP. Univ=idod AUI6nom. de 

San Luis PoIosf. Avila. Dr. Ml1luel 1'1.... 6. Zona Uni"milaria. San Luis Potosf 

78290, Mhico, and Alejandro RodLII·Uribo. Uni""n;idad AUl6noma de ~n!Ull'lI. 

Fa.cullad de: QufmiD:nInICd miJa:lJa 

")'11m!. The .oIvcnl conditionI invQtJ,lIo:d _~ 0I1hn>cdilf(nft pi"'!"Ahood 

0I~ (100:0_95:5 and 75:25. ~oI,/'voI)combined in. factorial design 

....lIh lhe fiye kyeI5"hoh=1 {O. 10.20.)0 and 4O'lIo w/wl u5 1....0 ~p"des wOIIId be caused by 

lhe differen« in lhei,- rq!rOduclivc cycle. Major fally ..,id CIIItlponenlS of I"""" 1....0 

~I'edel Were 14:0. 16:0. 18:I(n·9). 18:I(n-7), 20:I(n-II), 22:1(1'1-1I). 20:5(1'1-3) and 

22:6(n·3). The ral;O$ of 20:I(n-9).nd 22: l(n·ll) In 10lal fUlly lICids of A.per1

Page 1 .76 .. 1993 ANNUAL MEETING ABSTRACTS Session A ...

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