rezumat final - USAMV Cluj-Napoca

Cryptosporidiosis in calves and goat kids: research concerning diagnosis, epidemiology, and etiopathogenesis
spp. infection was diagnosed at such an early age, suggests a high contamination of this
area. The highest intensity of the parasitization with Cryptosporidium spp. was observed
between 1-2 weeks, where 54% of the cases presented average intensity, and 38%
presented elevated intensity, while at ages under one week and over three weeks, a low
intensity was observed (figure 3).
Fig. 2. Cryptosporidiosis prevalence (%) in
calves by age
Fig 3. Intensity of the parasitization with
Cryptosporidium spp. by age
The highest values of Cryptosporidium spp. infection incidence were observed at
the end of winter – beginning of spring, when they reached 70.21%, while at other times
during the year , the incidence was 16.80% ± 5.78%.
Through Cryptosporidium spp. micro-measurements it was detected the
elimination of one single type of oocyst measuring 4.51 ± 0.41 µm in lenght and 4.07 ±
0.33 µm in width, having a slightly oval shape.
Based on the analysis of SSU rRNA gene, trough nested PCR, it was detected in 8
samples, the amplification of a fragment of 638 bp, specific to the Cryptosporidium
genus. Out of the 8 samples, 5 were positive for Cryptosporidium parvum, human
genotype (62.5%), and 3 were positive for the Cryptosporidium parvum bovine genotype
(37.5%) (figure 4). No positive sample was revealed for the Cryptosporidium canis and
Cryptosporidium felis.
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