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TNR Handbook - Neighborhood Cats

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The <strong>Neighborhood</strong> <strong>Cats</strong> <strong>TNR</strong> <strong>Handbook</strong><br />

the primary goal is to stem overpopulation, neutering hundreds of cats should take<br />

priority over identifying 60 positives.<br />

But even if disease prevention in the feral population is considered an equal goal,<br />

spending the money to neuter hundreds of cats will stop the spread of disease a lot faster<br />

than plucking out 60 cats with viruses. Neutering eliminates kittens, the group most<br />

susceptible to catching disease because their immune systems are not fully developed.<br />

Neutering also eliminates mating behavior, a key transmission route of FeLV. In<br />

addition, deep bite wounds are the means by which FIV is transmitted among cats and<br />

neutering greatly reduces fighting among males.<br />

2. The tests normally administered to ferals are not reliable enough to make life or<br />

death decisions<br />

The FIV/FeLV test is really two different tests – one for each virus. Reliability issues<br />

depend on which virus is being tested for and what specific kind of test is being<br />

administered.<br />

For FIV and feral cats, most veterinarians and clinics use the ELISA test (Enzyme<br />

Linked Immunoabsorbent Assay). Often called a “snap” test, it produces results within<br />

minutes. The ELISA test for FIV detects whether antibodies to the FIV virus are present<br />

in the blood, not whether the virus itself is present. As a result, a positive test result does<br />

not necessarily mean the cat is infected. For cats less than six months of age, FIV<br />

antibodies may have been passed to them from their nursing mother, but not the virus.<br />

Another complication arises from the advent of the FIV vaccine. <strong>Cats</strong> who have received<br />

the vaccine will test positive for FIV, but only because the vaccine stimulated their<br />

immune system to form FIV antibodies.<br />

For FeLV, an ELISA snap test is again the most commonly administered to ferals.<br />

The ELISA test for FeLV determines whether the antigen of the virus is present in the<br />

blood. Thus, unlike the ELISA FIV test, the presence of the virus can be directly<br />

detected. The test though is sensitive and prone to false positives from mishandling. A<br />

classic example of mishandling occurs when the result given is “weak positive.” There is<br />

no such thing. Either the antigen is present in the blood or it’s not. “Weak positive”<br />

almost always indicates some type of testing error and the test should be re-administered.<br />

Even more troubling, just because a cat has the FeLV virus in their blood does not<br />

mean they will become permanently infected. Infection does not take permanent hold<br />

until the FeLV virus enters the cat’s white blood cells. If exposure to the virus was<br />

recent, which is always possible, the ELISA test would show a positive result, but the cat<br />

still might be able to fight off the disease. Only a second test can determine whether the<br />

virus has entered the white blood cells – this is the IFA test (Immunofluoresence Assay),<br />

also known as the Hardy test. The IFA/Hardy test must be performed at a lab and is more<br />

expensive. It’s almost never administered to feral cats absent special arrangements.<br />

Thus, to truly know if a cat is infected with FeLV, a positive ELISA test must be<br />

confirmed by a positive IFA/Hardy test. To diagnose FeLV based on a positive ELISA<br />

test alone is a faulty practice. Only if the positive ELISA test is confirmed with an IFA<br />

test or combined with other symptoms of illness can a reliable FeLV diagnosis be made.<br />

Given these facts, the practice of identifying positive cats based on one FIV/FeLV<br />

snap test inevitably leads to errors, resulting in the euthanasia of cats who were either not<br />

infected or would have fought off infection given the chance.<br />

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