QIAGEN News - Rede Pró-Fauna

New
Accelerate research and discovery using comprehensive siRNA sets
QIAGEN ® Human Library siRNA Sets provide hundreds of pre-designed, highly potent siRNA duplexes that are ready to use
for RNAi screening of complete families or classes of genes. The large number of carefully selected genes targeted by Human
Library siRNA Sets enables comprehensive yet detailed study of entire biochemical pathways or processes.
Human Library siRNA Sets provide:
■ Accelerated research and discovery — extensive gene coverage for
efficient screening of entire gene families
■ Cost-effective RNAi screening — optimally designed, pre-synthesized
siRNA sets allow economical, rapid high-throughput studies
■ The Golden Guarantee of 100% satisfaction — effective gene knockdown
with satisfaction guaranteed, or free redesign and replacement
■ Highly potent siRNA generated using the world’s best siRNA design
algorithm — the HiPerformance design algorithm is integrated with a
proprietary homology analysis tool for optimal siRNA design
Sets are currently available for the study of kinases (646 genes), G protein-coupled receptors
(471 genes), and apoptosis-related factors (418 genes). QIAGEN scientists use the most up-to-date
public databases to develop content for Human Library siRNA Sets, including the National Center
for Biotechnology Information (NCBI), Ensembl, Swiss-Prot, the UCSC Genome browser, and
InterPro (European Bioinformatics Institute). Human Library siRNA Sets offer the widest coverage of
gene families available at the time of design. Two highly potent, high-purity siRNA duplexes are
supplied for each gene target. Each plate also includes control wells that contain a scrambled
non-silencing siRNA and an siRNA directed against GFP, and empty wells for customer-defined
controls (e.g., positive controls or mock transfections in the absence of siRNA). Complete,
restriction-free sequence information is provided at no extra cost.
Custom siRNA Set
With Custom siRNA Sets, researchers are free to choose which genes they wish to target. Duplexes
in Custom siRNA Sets come with the Golden Guarantee of 100% satisfaction, are designed using
the same cutting-edge algorithm as Human Library siRNA Sets, and also offer:
■ Flexibility and scalability — target the specific type and number of genes that your research
requires
World’s most advanced siRNA design generates highly potent siRNA
All siRNA duplexes in Human Library and Custom siRNA Sets are designed using the revolutionary
HiPerformance design algorithm licensed from Novartis Pharmaceuticals. This algorithm is based
on the largest independent study of siRNA functionality to date, in which the gene silencing
efficiency was analyzed for over 3300 duplexes directed against 33 targets. The HiPerformance
design algorithm is integrated with a proprietary homology analysis tool and a comprehensive
gene database, delivering siRNA that provides maximum gene silencing efficiency with minimal
off-target effects. The efficacy of the algorithm has been proven by analysis of hundreds of siRNA
duplexes directed against multiple genes (Figure 1).
74 www.qiagen.com QIAGEN News 2004 Issue 3