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Faster high-throughput manual and automated PCR cleanup using
the MinElute ® 96 UF PCR Purification Kit
The MinElute 96 UF PCR Purification Kit provides fast, high-throughput PCR purification, in a simple manual or fully
automated procedure (see flowchart). An optimized ultrafiltration membrane delivers fast flow rates and high, reproducible
recovery, even of smaller DNA fragments (Figure 1). The advanced plates in the kit are designed to allow final elution
volumes as low as 20 µl in the manual procedure, delivering highly concentrated, purified DNA in just 13 minutes. A purple
O-ring in each well allows researchers to see eluates more easily and pipet more accurately.
The MinElute 96 UF PCR Purification Kit offers:
■ Minimal elution volumes — high DNA concentrations in as little as 20 µl
■ Fast, cost-effective procedure — well suited for high-throughput projects
■ Fully automatable processing — walkaway processing on BioRobot ®
workstations and other SBS-compatible automated systems (Figure 2)
■ High, reproducible recovery — for fragments >100 bp
Efficient, high-throughput PCR cleanup
PCR products are loaded into the wells of ultrafiltration plates and a
vacuum is applied. While small molecules such as primers, salts, and
unincorporated nucleotides run through the membrane, PCR products
≥100 bp are retained. Purified PCR products are eluted directly from the
surface of the membrane in small volumes (as little as 20 µl in the manual
procedure), leading to highly concentrated eluates. Purified DNA
fragments are ready for direct use in all applications, including sequencing,
microarray analysis, ligation, and restriction digestion.
MinElute 96 UF PCR Purification Plates conform to SBS (Society for
Biomolecular Screening) standards and offer two convenient handling
options. Plates can be processed manually using a commercial vacuum
manifold (e.g., QIAvac Multiwell, cat. no. 9014579) or on the BioRobot
3000 or 8000 or other automated workstations (Figure 2).
Walkaway Processing
Ordering Information
Product Contents Cat. no.
MinElute 96 UF PCR 4 MinElute 96 UF PCR Purification Plates 28051
Purification Kit (4)*
* Larger kit sizes available: please inquire.
Figure 2 Automated processing on the BioRobot 3000
workstation.
Efficient Purification of Concentrated DNA
m Q M III M Q M III M m
– 500 bp
– 100 bp
Figure 1 DNA fragments (100 bp and 500 bp) were
purified using either MinElute 96 UF PCR Purification Plates
(Q) or ultrafiltration-based kits from other suppliers (Supplier
MIII and Supplier M). Aliquots (5 µl) of the eluate were
analyzed by agarose-gel electrophoresis. m: markers.
QIAGEN News 2004 Issue 3 www.qiagen.com 65