Sample Preparation Strategies for Water Analysis
Sample Preparation Strategies for Water Analysis
Sample Preparation Strategies for Water Analysis
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Optimized SPE Protocol<br />
<strong>for</strong> River <strong>Water</strong><br />
Oasis ® WAX sorbent was<br />
selected <strong>for</strong> these analytes<br />
Logic: PFOA pKa ~1<br />
PFOS pKa < 1<br />
Oasis ® WAX<br />
N<br />
N<br />
H<br />
N O<br />
H<br />
N<br />
N<br />
H H<br />
mixed-mode weak anion-exchange<br />
pKa ~6<br />
+<br />
+<br />
Conditions <strong>for</strong> Oasis ® WAX 3 cc 60mg cartridges<br />
Oasis ® WAX<br />
Optimized Protocol 1<br />
Prepare <strong>Sample</strong><br />
pH 3<br />
Condition<br />
2 mL methanol/2 mL water<br />
Load<br />
200 mL<br />
Wash #1<br />
1 mL 2% Formic acid<br />
Elute 1 (Wash #2)<br />
2 mL methanol<br />
Elute 2<br />
2 mL 1% conc. ammonia in<br />
10:90 methanol/MTBE<br />
<strong>Sample</strong>s were evaporated and<br />
reconstituted in 0.15 mL mobile phase<br />
©2007 <strong>Water</strong>s Corporation 24
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