Genetic characteristics of field and attenuated rabies viruses ... - Evira

Content
List of abbreviations ....................................................................................................... 5
Abstract............................................................................................................................ 6
List of original publications............................................................................................ 8
1. Literature review........................................................................................................ 9
1.2. Rabies virus characteristics ................................................................................ 9
1.2.1. Classification ................................................................................................... 9
1.2.2. Structure of virion, genome, and proteins of rabies virus............................... 10
1.3. Virus-host interaction ........................................................................................ 12
1.3.1. Pathogenesis................................................................................................. 12
1.3.2. Clinical signs.................................................................................................. 13
1.3.3. Pathological changes .................................................................................... 15
1.4. Laboratory diagnosis of rabies .......................................................................... 17
1.4.1. Detection of viral antigen ............................................................................... 17
1.4.2. Detection of viral genome.............................................................................. 18
1.4.3. Virus isolation ................................................................................................ 19
1.4.4. Detection of virus neutralizing antibodies ...................................................... 19
1.5. Rabies epidemiology......................................................................................... 21
1.5.1. Rabies situation world-wide........................................................................... 21
1.5.2. Rabies situation in Europe and in Russia ...................................................... 22
1.5.3. Models of rabies epidemics ........................................................................... 24
1.6. Fixed strains of rabies virus .............................................................................. 25
1.6.1. Laboratory strains.......................................................................................... 25
1.6.2. Vaccine strains and anti-rabies vaccines....................................................... 25
1.7. Molecular biology of rabies viruses ................................................................... 30
1.7.1. Antigenic characterization with monoclonal antibodies.................................. 30
1.7.2. Genetic characterization of rabies virus......................................................... 31
1.7.3. Entire genome sequencing of vaccine strains ............................................... 33
2. Aims of the study .................................................................................................... 35
3. Materials and methods............................................................................................ 36
3.1. Field rabies virus isolates.................................................................................. 36
3.2. Vaccine and laboratory strains.......................................................................... 36
3.3. Cell cultures ...................................................................................................... 36
3.4. Monoclonal antibodies ...................................................................................... 37
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