Genetic characteristics of field and attenuated rabies viruses ... - Evira

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The entire genome sequence of the Russian vaccine strain RV-97 was aligned with 10 entire genome sequences of vaccine the strains obtained from the Gene Bank database. For phylogenetic analysis, the nucleotide sequences of the 5 viral genes of each strain were translated into protein sequences and joined to one sequence in the original order. The bat virus SHBRV-18 was taken as an out-group and the minimum evolution phylogenetic tree was rooted to this virus. The phylogenetic tree was bootstrapped with 1000 replicates (paper V). 41
4. Results 4.1. FAT and cell culture inoculation test All samples tested by the diagnostic FAT were rabies positive. The samples were further analyzed with indirect FAT on glass slides (field viruses from the Russian Federation) or in cell cultures (archival viruses from Finland and Estonia and vaccine strains). In some cases, cell culture inoculation tests and RT-PCR and nucleotide sequencing were conducted; these results were also positive. 4.2. Antigenic characteristics of field rabies viruses (papers I, II and III) Five antigenic variants were found among the 233 virus samples tested by means of the reaction pattern with the panel of mAbs (Table 1). Table 1. Antigenic variants of rabies virus isolates using mAbs Antigenic Genot Reaction with Mab variant/Region ype W-239.17 W-187.5 W-187.11.2 MW- 187.6.1 P-41 I. Bryansk, Estonia, + The results of this study + + + + Finland, Kursk, Pskov, Tver II. Estonia + - + + + III. Bashkiriya, Belgorod, + + + + - Bryansk, Krasnodar, Kursk, Mordoviya, Novosibirsk, N.Novgorod, Orel, 1 Penza, Tver, Vladimir, Voronezh IV. Bashkiriya, Krasnodar, Mordoviya, Moscow, N. Novgorod, Ryazan, Tver, Tula, Vladimir + - + + - V. Tver, Moscow + - + - - Results from the literature Arctic 1 1 + + + + + European fox 2 1 + + + + - West-European 2 1 + - + + - Logos bat 3, 4 2 + - - + - Mocola 3, 4 3 + - - + - Duvenhage 3, 4 4 + - - + - EBL1 2 5 + - - - - EBL2 1 6 + - - + - 1 2 3 4 Schneider et al., 1985; Cox et al., 1992; Umoh et al., 1990; Mebatsion et al., 1992. 42
Page 1 and 2: Evira Research Reports 2/2008 Artem
Page 3 and 4: Supervised by Anita Huovilainen, Ph
Page 5 and 6: 3.5. Laboratory techniques.........
Page 7 and 8: Abstract Rabies is a fatal disease
Page 9 and 10: List of original publications I. Me
Page 11 and 12: 1.2.2. Structure of virion, genome,
Page 13 and 14: al., 1989; Benmansour et al., 1991)
Page 15 and 16: Prodromal period - the first 1 to 3
Page 17 and 18: A. C. Figure 4. A&B. Perivascular c
Page 19 and 20: the skin biopsies (Bryceson et al.,
Page 21 and 22: cells, murine monocytic P388D1 and
Page 23 and 24: 1.5.2. Rabies situation in Europe a
Page 25 and 26: It is well established that the mai
Page 27 and 28: widely used oral anti-rabies vaccin
Page 29 and 30: have to be surpassed. Since then, s
Page 31 and 32: There are two ways of distributing
Page 33 and 34: The RT-PCR amplification, nucleotid
Page 35 and 36: into the M13 vectors. Conzelmann et
Page 37 and 38: 3. Materials and methods 3.1. Field
Page 39 and 40: was added to all mixtures and these
Page 41: The total RNA was purified from the
Page 45 and 46: 4.4. Phylogenetic analysis and mole
Page 47 and 48: group differ from those observed in
Page 49 and 50: The vaccine strains RV-97 and SAD B
Page 51 and 52: 5. Discussion Rabies is a serious v
Page 53 and 54: as from non-infectious diseases (e.
Page 55 and 56: Nevertheless, the major rivers rema
Page 57 and 58: antigenic sites (epitopes) of the G
Page 59 and 60: The data included in this thesis ca
Page 61 and 62: 7. Заключение (concluding
Page 63 and 64: 8. Acknowledgments The work for thi
Page 65 and 66: 19. Beauregard, M., Boulanger, P.,
Page 67 and 68: 46. Brozka, K., Finke, S., Conzelma
Page 69 and 70: 75. Dietzschold, B., Lafon, M., Wan
Page 71 and 72: 107. Hellenbrand, W., Meyer, C., Ra
Page 73 and 74: 141. Kumar, S., Tamura, K., Nei, M.
Page 75 and 76: 169. McElhinney, L.M., Marston, D.,
Page 77 and 78: 197. Niin, E., Laine, M., Partel, A
Page 79 and 80: 230. Selimov, M.A., Botvinkin, A.D.
Page 81 and 82: 262. Tollis, M., Buonavoglia, C., T
Page 83 and 84: 295. Wunner, W.H., 1991. The chemic
Page 85 and 86: J. Vet. Med. B 51, 94-96 (2004) Ó
Page 87 and 88: 96 A. E. Metlin et al. Mu¨ ller, T
Page 89 and 90: 125•Mise globale 23.2.2006 9:58 P
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125•Mise globale 23.2.2006 9:58 P
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CASE REPORT REFEREED Imported Case
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Figure 1. Neighbor-joining phylogen
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Paper IV
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study. The reverse transcription-po
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Table 1 (continued) Code Origin Ani
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Fig. 2. Geographical distribution o
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Besides rivers, mountain ranges wer
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Paper V
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104 105 106 107 108 109 110 111 112
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154 155 156 157 158 159 Q5 160 161
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Finnish Food Safety Authority Evira
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