rDNA-ITS2 Identification of Hyalomma, Rhipicephalus ... - HIKARI Ltd

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230 M. Abdigoudarzi et al Figure3- Phylogenetic tree using UPGMA method (CLC Sequence Viewer)
DNA-ITS2 identification 231 4. Discussion Murrell et al (2001) studied totally information into the evolution of ticks, and they noted in their studies that the size of ITS2 in ticks should be near 1kb but the aligned region for Haemaphysalis spp. was 422 bp. This problem was noted in our study too. They also noticed that no sequences were available for ITS2 for species from the genus Hyalomma on that time. The problem is more prominent for Hyalomma spp. (figure 2, 3 and table 3) but we could finally amplify the ITS2 segment and complete sequence of 829bp for B. annulatus and it was successfully aligned. There are discriminative bands for Rhipicephalus and Boophilus genera on agarose gel (1%) after electrophoresis and they could be identified just with respect to a ladder marker (figure2). 1 2 3 4 5 6 7 1 2 3 4 5 6 7 M Figure 1- Sample agarose gel electrophoresis of PCR products related to ITS2 amplification for Iranian Ixodidae. 1- Hyalomma marginatum 2- Boophilus annulatus(Accession no. AY702974)(829bp) 3- Hyalomma marginatum 4- Hyalomma anatolicum anatolicum 5- Hyalomma anatolicum anatolicum 6- Rhipicephalus sanguineus(not amplified) 7- Rhipicephalus sanguineus
Page 1 and 2: Advanced Studies in Biology, Vol. 3
Page 3 and 4: DNA-ITS2 identification 223 annulat
Page 5 and 6: Species R. turanicus R. turanicus R
Page 7 and 8: DNA-ITS2 identification 227 Bioer T
Page 9: DNA-ITS2 identification 229 The 5 D
Page 13 and 14: DNA-ITS2 identification 233 There w
Page 15 and 16: DNA-ITS2 identification 235 [2] S.
Page 17 and 18: DNA-ITS2 identification 237 [25] M.

rDNA-ITS2 Identification of Hyalomma, Rhipicephalus ... - HIKARI Ltd

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