Mycoplasma pneumoniae Chlamydophila pneumoniae - Mikrogen

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DIAGENODE MYCOPLASMA PNEUMONIAE / CHLAMYDOPHILA PNEUMONIAE REAL-TIME PCR USER MANUAL
IV . PCR profile
> ABI 7000-7300-7500-7900 / Roche Lc480 / Biorad iCycler-IQ5-CFX96 / Stratagene
MX3000P-3005P / Qiagen Rotor-Gene / Cepheid Smartcycler II (on request)
Stage Description
1 2 minutes at 50°C (1 cycle)
2 10 minutes at 95°C (1 cycle)
3 Cycle program (45 cycles)
Step 1: 15 seconds at 95°C
Step 2: 60 seconds at 60°C
NB:
For the step 2 from the stage 3, it is highly recommended to decrease the ramping rate
(°C/s) by 50%.
> Roche Lc2.0
Stage Description
1 10 minutes at 95°C (1 cycle)
2 Cycle program (45 cycles)
Step 1: 10 seconds at 95°C
Step 2: 40 seconds at 60°C
Step 3: 1 second at 72°C
3 30 seconds at 40°C (1 cycle)
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