Mycoplasma pneumoniae Chlamydophila pneumoniae - Mikrogen

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DIAGENODE MYCOPLASMA PNEUMONIAE / CHLAMYDOPHILA PNEUMONIAE REAL-TIME PCR USER MANUAL
> Stratagene MX3000P-3005P
Detector’s name Reporter Quencher
Mycoplasma pneumoniae FAM FAM (none)
Chlamydophila pneumoniae Yellow Dye Vic/Hex (none)
DIA-EIC/DNA(Cy5)-050 Cy5 Cy5 (none)
NB:
In Filter Set Gain Settings:
• FAM: select x8
• Hex/JOE (Yellow Dye): select x2
• Cy5: select x4
> Qiagen Rotor-Gene
Detector’s name Channel
Mycoplasma pneumoniae FAM Green
Chlamydophila pneumoniae Yellow Dye Yellow
DIA-EIC/DNA(Cy5)-050 Cy5 Red
NB:
Perform an optimization at 60 degrees at the beginning of each run for the green and red
channels.
> Cepheid SmartCycler II
Detector’s name Reporter Quencher
Mycoplasma pneumoniae FAM FAM (none)
Chlamydophila pneumoniae Yellow Dye Alexa532 (none)
DIA-DNA/EIC(TR)-050 Texas red Texas red (none)
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